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Germplasm maintenance

  1. Sept 2012 Germplasm Maintenance Maintenance of germplasm includes Monitoring seed viability ™™ Seed viability of conserved germplasm is tested at regular intervals, five years for medium-term storage and ten years for long-term storage Monitoring seed quantity ™™ Seed quantity in medium-term store is recorded every year Testing the viability of conserved germplasm in the seed biology laboratory. Regeneration of germplasm ™™ Accessions are regenerated when seed viability and seed quantity are critical (<85% viability or <1/4 of total quantity). ™™ Regeneration is carried out in the postrainy season for securing high quality seed. ™™ Cereals are sown using tractor mounted 4-cone planters, while legumes are hand sown. ™™ Maintaining genetic integrity of accessions during regeneration is an important activity in cross- pollinated crops. ™™ Maximum care is taken to avoid admixtures while sowing, harvesting, threshing, seed processing and transfer. Sowing cereal germplasm for regeneration. Preparing for tractor planting of cereal germplasm. Field view of sorghum germplasm regeneration. Clasping craft paper bag to identify plants. Field view of pearl millet germplasm regeneration. Field view of chickpea germplasm regeneration. Harvesting and threshing of individual plants of chickpea while regeneration. Perennial chickpea species grown for regeneration in glasshouse. Pigeonpea accessions grown for regeneration under insect proof cages. Wild relatives of pigeonpea grown for regeneration in the field genebank. Field view of groundnut germplasm regeneration. Poor pod producing groundnut germplasm regenerated in glasshouse. Self-pollinated small millets grown for regeneration. Special facility created to regenerate wild relatives of groundnut by growing in RCC rings. ™™ Pollination control to avoid contamination due to foreign pollen is important in cross pollinating crops. ™™ Equal quantity of seeds from each plant is bulked to reconstitute the accession. ™™ Species that do not produce seeds are maintained as live plants in glasshouse or in a field genebank. ™™ A special facility of reinforced cement concrete (RCC) rings has been created to regenerate wild relatives of groundnut. Cluster bagging in pearl millet germplasm.
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