Genomics for converting 3-lines to 2-lines hybrid system in pigeonpea By Dr Lekha T Pazhamala, ICRISAT
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This breakthrough research will pave the way for two line hybrid breeding system, Molecular mechanisms for fertility reversion deduced and application of auxin in reverting sterility validated.
Genomics for converting 3-lines to 2-lines hybrid system in pigeonpea By Dr Lekha T Pazhamala, ICRISAT
Genomics for converting 3-lines to
2-lines hybrid system in pigeonpea
Lekha T Pazhamala
DST Women Scientist
Pigeonpea (Cajanus cajan)
ICPH 2671
Local check
Food legume with diversified uses such as
food, feed, fodder and fuel
India : Largest producer, consumer, importer
Commodity crop
Myanmar, Tanzania, Kenya, Malawi,
Uganda, Mozambique
Pigeonpea hybrids
World’s first commercial legume
hybrid system
Hybrids yielding upto 40% over
varieties
Exploring two-lines hybrid system
Environment-sensitive
male sterile line
Restorer lineCytoplasmic
male sterile line
Restorer
line
8 Environment-Sensitive Male Sterile (ESMS) lines
Further evaluation in two years in fields
Phenotypic
studies
Transcriptomic
studies
What is happening at the
cellular level?
What genes are involved in
fertility reversion?
What factors cause fertility
reversion?
Three lines evaluated under controlled conditions
Cytological
studies
Evaluation & characterization of ESMS lines
• Photoperiod
• Day temperature
• Night temperature
• Light intensity
• Humidity
Key environmental factor: Day temperature
30°C day
16°C night
13 h day length
11 h day length
8 h day length
Sterile
Photoperiod
Night temperature
Light intensity
Humidity
30°C day
13h day length
16°C night
10°C night
8°C night
12°C night
Sterile
12°C night
11h day length
30°C day
25°C day
18°C day
Sterile
Fertile
Summary
• Molecular mechanisms for fertility reversion deduced
• A stable thermo-sensitive male sterile line identified
• 24 °C as a threshold day temperature
• Tetrad and microspore as critical stages
• Application of auxin in reverting sterility validated
• This breakthrough research will pave the way for two line
hybrid breeding system
Next steps
• Evaluation of identified line in targeted temperature zones
for deployment in two-lines hybrid breeding system
• Candidate genes/genomic region responsible for fertility
reversion
Rajeev Varshney
Rachit Saxena
Anu Chitikineni
Prasad Bajaj
KB Saxena
CV Sameer Kumar
Anupama Hingane
Acknowledgements
Blue Sky Research Initiative from ICRISAT
Thank You