Fungus project WUR

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Fungus project WUR

  1. 1. Mold’ly going where no man has gone before Mold Track `n Trace iGEM 2011 project proposal – Wageningen UR 2011
  2. 2. <ul><ul><li>Following a signal (eGFP) through a hypha </li></ul></ul><ul><ul><li>Diffusion of signal carrier through septa </li></ul></ul>A. nidulans
  3. 3. Project - Hyphae
  4. 4. <ul><ul><li>Initial stimulation </li></ul></ul>Project - Hyphae
  5. 5. <ul><ul><li>Initial stimulation </li></ul></ul><ul><ul><li>First signal produced </li></ul></ul><ul><ul><li>Carrier buildup </li></ul></ul>Project - Hyphae
  6. 6. <ul><ul><li>Initial stimulation </li></ul></ul><ul><ul><li>First signal produced </li></ul></ul><ul><ul><li>Carrier buildup </li></ul></ul><ul><ul><li>Carrier diffuses </li></ul></ul><ul><ul><li>Process start again in cell N+1 </li></ul></ul>Project - Hyphae
  7. 7. <ul><ul><li>Initial stimulation </li></ul></ul><ul><ul><li>First signal produced </li></ul></ul><ul><ul><li>Carrier buildup </li></ul></ul><ul><ul><li>Carrier diffuses </li></ul></ul><ul><ul><li>Process start again in cell N+1 </li></ul></ul><ul><ul><li>etc. </li></ul></ul>Project - Hyphae
  8. 8. Project - Colony
  9. 9. Project - Colony
  10. 10. Project - Colony
  11. 11. Project - Colony
  12. 12. Project - Colony
  13. 13. Project - Colony
  14. 14. Proposed mechanisms XlnR* eGFP-S vac xpna xpna XlnR* XlnR* XlnR* XlnR* XlnR* XlnR* XlnR*
  15. 15. Proposed mechanisms GAL4 eGFP-S vac UAS gal4 GAL4 UAS gal4
  16. 16. GAL4 regulation 3 c 4 4 4 4 4 4 10bp 10bp 80 80 80 80 80 80 3 c 3 c 3 c
  17. 17. Combination 3 c 3 c 4 4 80 80 XlnR* XpnA XpnA GAL3 c eGFP-S vac 3 c
  18. 18. S.W.O.T
  19. 19. <ul><ul><li>New model organism, A. nidulans </li></ul></ul><ul><ul><li>Visual signal (GFP) </li></ul></ul><ul><ul><li>System can be subdivided (modular) </li></ul></ul><ul><ul><li>Proposed RR have been used before </li></ul></ul><ul><ul><li>Some are even BioBricked </li></ul></ul><ul><ul><li>In house knowledge about A. nidulans </li></ul></ul>S .C.O.T
  20. 20. <ul><ul><li>Keeping noise from ruining system </li></ul></ul><ul><ul><li>Tuning the system </li></ul></ul><ul><ul><li>Lots of constructs need to be made </li></ul></ul>S. C .O.T
  21. 21. <ul><ul><li>Tuning and noise reduction using the GAL system </li></ul></ul><ul><ul><li>Opportunity to gain fundamental knowledge about A. nidulans hyphae </li></ul></ul><ul><ul><li>Opportunity to broaden the scope of synthetic biology BioBricks </li></ul></ul>S.C. O .T
  22. 22. <ul><ul><li>Long period from transformation until measuring of results, 1 Month. </li></ul></ul><ul><ul><li>Unknown synthetic territory </li></ul></ul><ul><ul><li>Eukaryote chassis </li></ul></ul>S.C.O. T
  23. 23. ? Questions

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