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Creating  Xylella  Resistant  Grapevines  by Conventional Breeding <ul><li>Andy Walker, Alan Tenscher, Summaira Riaz </li>...
History of Breeding for PD Resistance <ul><li>Muscadine grapes have excellent PD resistance, but physically and geneticall...
PD   Resistance of Olmo’s Mexico Collections 9R 7I 8S 4R,2I,1S 8R,0S 5R 4I 1S 1R,0S
Sources of Xf resistance <ul><li>b43-17 – ( V. arizonica/candicans ) from Monterrey, Mexico – resistance is inherited as a...
Sources of Xf resistance <ul><li>Markers linked to  PdR1  from b43-17 have different alleles in b40-14 and b42-26 </li></u...
Mapping and Characterizing PD Resistance <ul><li>The  PdR1  resistance locus exists in  V. arizonica  b43-17 </li></ul><ul...
Mapping and Characterizing PD Resistance <ul><li>Fine scale mapping has positioned  PdR1  within 1cM of markers on LG 14 <...
 
8909-19 S  8909-08 R
Breeding Objectives <ul><li>Breed PD resistant wine grapes by backcrossing Xf resistant selections to high quality  V. vin...
Breeding Objectives <ul><li>Develop large seedling populations at the 94% and 97%  vinifera  level in many high quality  v...
Backcross Breeding <ul><li>Begin with a resistance source crossed to  V. vinifera </li></ul><ul><li>–  That F1 is 50%  V. ...
2008 Progress at 93.75%  V. vinifera ( mBC3) <ul><li>Planted about 400 seedlings (some fruited!), will produce 97% (mBC4) ...
Progress at 93.75%  V. vinifera ( mBC3) F8909-08 =  V. rupestris  x  V. arizonica  b43-17 504-49 = B90-116 x 8909-08 ( PdR...
2008 crosses to produce (A) = 93.75%  vinifera   PdR1b  (F8909-08) and (B) = 87.5%  vinifera PdR1a  resistance (F8909-17).
2008 crosses to produce: A) = 90.6%  vinifera   PdR1b  (F8909-08) with  Run1  powdery mildew resistance; and B) = 87.5%  v...
2008 rootstock crosses to combine PD and nematode resistance.
2008 Crosses: 87.5%  vinifera  progeny (A)  PdR1a  (F8909-17); (B)  arizonica  b40-14 (06339); (C)  shuttleworthii  (0098-...
87.5%  V. vinifera  / 50% Chardonnay
2008 Winegrape Evaluations Reference cultivars and select 87.5%  vinifera  progeny with  PdR1 .
2008 Winegrape Evaluations Juice and wine analysis courtesy of ETS Laboratories,  St. Helena, CA.
2008 Wine Evaluations  87.5%  vinifera Tasting results of 2007 wines (1= poor to 5 = very good).  There were 10 faculty an...
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Creating Xylella Resistant Grapevines by Conventional Breeding - Andy Walker - Pierce's Disease Conference 2008

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Creating Xylella Resistant Grapevines by Conventional Breeding

Andy Walker, Alan Tenscher, Summaira Riaz
& David Ramming

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Creating Xylella Resistant Grapevines by Conventional Breeding - Andy Walker - Pierce's Disease Conference 2008

  1. 1. Creating Xylella Resistant Grapevines by Conventional Breeding <ul><li>Andy Walker, Alan Tenscher, Summaira Riaz </li></ul><ul><li>& David Ramming </li></ul>Support from CDFA PD/GWSS Board, the AVF, and the Louis P. Martini Endowed Chair Funds
  2. 2. History of Breeding for PD Resistance <ul><li>Muscadine grapes have excellent PD resistance, but physically and genetically hard to introgress resistance into vinifera </li></ul><ul><li>Bunch grape breeding in the southeastern US - (Blanc du Bois, BD5-1) has been hindered by intense fungal disease pressure and quantitative inheritance of PD resistance from conventional sources </li></ul>
  3. 3. PD Resistance of Olmo’s Mexico Collections 9R 7I 8S 4R,2I,1S 8R,0S 5R 4I 1S 1R,0S
  4. 4. Sources of Xf resistance <ul><li>b43-17 – ( V. arizonica/candicans ) from Monterrey, Mexico – resistance is inherited as a single dominant gene </li></ul>
  5. 5. Sources of Xf resistance <ul><li>Markers linked to PdR1 from b43-17 have different alleles in b40-14 and b42-26 </li></ul><ul><li>Many more V. arizonica and V. girdiana forms have been collected for testing </li></ul>
  6. 6. Mapping and Characterizing PD Resistance <ul><li>The PdR1 resistance locus exists in V. arizonica b43-17 </li></ul><ul><li>All progeny from this plant are resistant to Xf; the F8909 pop. ( V. rupestris x b43-17) </li></ul><ul><li>F8909-08 and F8909-17 have been used in breeding and mapping Xf resistance </li></ul><ul><li>D8909-15 x F8909-17 = 9621 population </li></ul>
  7. 7. Mapping and Characterizing PD Resistance <ul><li>Fine scale mapping has positioned PdR1 within 1cM of markers on LG 14 </li></ul><ul><li>PdR1 from F8909-17 and F8909-08 map differently and represent either chromosome of the PdR1 locus – PdR1a and PdR1b from b43-17 </li></ul>
  8. 9. 8909-19 S 8909-08 R
  9. 10. Breeding Objectives <ul><li>Breed PD resistant wine grapes by backcrossing Xf resistant selections to high quality V. vinifera wine grape cultivars. </li></ul><ul><li>Characterize Xf resistance and wine grape quality traits (color, tannin, ripening dates, flavor, productivity, etc). </li></ul><ul><li>Work with David Ramming to accomplish the same goals with table and raisin grapes. </li></ul>
  10. 11. Breeding Objectives <ul><li>Develop large seedling populations at the 94% and 97% vinifera level in many high quality vinifera wine and table backgrounds </li></ul><ul><li>Intercross advanced high quality selections with Xf resistance from multiple sources </li></ul><ul><li>Introgress in powdery mildew resistance </li></ul><ul><li>Develop genetic markers for fruit and wine quality </li></ul>
  11. 12. Backcross Breeding <ul><li>Begin with a resistance source crossed to V. vinifera </li></ul><ul><li>– That F1 is 50% V. vinifera </li></ul><ul><li>– Cross back to V. vinifera – 75% mBC1 </li></ul><ul><li>– Cross back to V. vinifera – 87.5% mBC2 </li></ul><ul><li>– Cross back to V. vinifera – 93.75% mBC3 </li></ul><ul><li>– Cross back to V. vinifera – 96.89% mBC4 </li></ul><ul><li>– 3 yr cycle with marker-aided selection (MAS) </li></ul>
  12. 13. 2008 Progress at 93.75% V. vinifera ( mBC3) <ul><li>Planted about 400 seedlings (some fruited!), will produce 97% (mBC4) seed in 2009 </li></ul><ul><li>Propagated 8 strong seedlings (6 to 7 vine reps) to wide spacings and Wye trellis for wine lots in Fall 2009 </li></ul>
  13. 14. Progress at 93.75% V. vinifera ( mBC3) F8909-08 = V. rupestris x V. arizonica b43-17 504-49 = B90-116 x 8909-08 ( PdR1b ) A81-17 = A38-17 x 504-49 0502-48 = A81-138 x Chardonnay 0505-01 = A81-138 x Cabernet Sauvignon
  14. 15. 2008 crosses to produce (A) = 93.75% vinifera PdR1b (F8909-08) and (B) = 87.5% vinifera PdR1a resistance (F8909-17).
  15. 16. 2008 crosses to produce: A) = 90.6% vinifera PdR1b (F8909-08) with Run1 powdery mildew resistance; and B) = 87.5% vinifera PdR1b resistance (F8909-08) with vinifera PM resistance.
  16. 17. 2008 rootstock crosses to combine PD and nematode resistance.
  17. 18. 2008 Crosses: 87.5% vinifera progeny (A) PdR1a (F8909-17); (B) arizonica b40-14 (06339); (C) shuttleworthii (0098-03 – 75% vinifera and 04394 – 50% vinifera ); and (D) arizonica/girdiana b42-26 (50% vinifera progeny).
  18. 19. 87.5% V. vinifera / 50% Chardonnay
  19. 20. 2008 Winegrape Evaluations Reference cultivars and select 87.5% vinifera progeny with PdR1 .
  20. 21. 2008 Winegrape Evaluations Juice and wine analysis courtesy of ETS Laboratories, St. Helena, CA.
  21. 22. 2008 Wine Evaluations 87.5% vinifera Tasting results of 2007 wines (1= poor to 5 = very good). There were 10 faculty and staff tasters. Cabernet Sauv., Midsouth and U0502-07 and -01 not fermented in 2008
  22. 23. Thanks!

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