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Penn State Tomato Breeding Program

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Penn State Tomato Breeding Program

  1. 1. Tomato Genetics and Breeding Research at Penn State Majid R. Foolad Department of Horticulture, and The Intercollege Graduate Degree Programs in Genetics and Plant Physiology The Pennsylvania State University
  2. 2. Goals <ul><li>Identify genetic sources for desirable traits </li></ul><ul><li>Discern genetic basis of traits of interest </li></ul><ul><li>Develop genetic markers and maps </li></ul><ul><li>Identify genes or QTLs for traits of interest </li></ul><ul><li>Develop tomato breeding lines and cultivars using a combination of traditional and marker-assisted breeding </li></ul>
  3. 3. Traits of Interest <ul><li>Disease Resistance </li></ul><ul><ul><li>In particular Early Blight (EB) and Late Blight (LB) Resistance </li></ul></ul><ul><li>Fruit Quality </li></ul><ul><ul><li>In particular Lycopene (LYC) Content </li></ul></ul><ul><li>Abiotic Stress Tolerance </li></ul><ul><ul><li>In particular Drought Tolerance </li></ul></ul><ul><li>Adaptation to US Northeast Growing Conditions </li></ul>
  4. 4. Goal 1 Identify Genetic Resources <ul><li>Screened over 300 wild accessions of tomato and identified: </li></ul><ul><ul><li>Sources of EB resistance </li></ul></ul><ul><ul><li>Sources of LB resistance </li></ul></ul><ul><ul><li>Sources of high fruit LYC content </li></ul></ul><ul><ul><li>Sources of tolerance to abiotic stresses (e.g. drought tolerance, salt tolerance, etc.) </li></ul></ul><ul><li>Screening conducted under field, greenhouse, high tunnel and growth chamber conditions </li></ul>
  5. 5. Screening for EB Resistance in Greenhouse
  6. 6. Screening for LB Resistance in High Tunnel
  7. 7. Screening for Drought Tolerance in Greenhouse
  8. 8. Screening for Salt Tolerance in Greenhouse
  9. 9. Genetic Source of High Fruit Lycopene Content Identified
  10. 10. Goal 2 Discern Genetic Basis of Traits <ul><li>Conducted various genetics experiments to determine inheritance of and correlation between the traits of interest </li></ul>
  11. 11. Sensitive Parent X Tolerant Parent F 1 X Control (n = 400) Cold (n = 400) Salt (n = 400) Treatment: Proportion selected: Family size: 5% 20 F 3 Families Control Cold Salt 480 480 480 Treatment: 5% X Control Cold Salt 480 480 480 5% X Control Cold Salt 480 480 480 Progeny: X 20 F 3 Families 20 F 3 Families F 2 Estimation of Heritability and Correlation between Traits
  12. 12. Goal 3 Develop Genetic Markers and Maps <ul><li>Identified numerous polymorphic markers based on different parents </li></ul><ul><li>Developed several new genetic maps of tomato based on various crosses, including: </li></ul><ul><ul><li>S. lycopersicum X S. pimpinellifolium LA722 </li></ul></ul><ul><ul><ul><li>BC map </li></ul></ul></ul><ul><ul><ul><li>RIL map </li></ul></ul></ul><ul><ul><li>S. lycopersicum X S. habrochaites PI126445 </li></ul></ul><ul><ul><ul><li>BC map </li></ul></ul></ul><ul><ul><li>S. lycopersicum X S. pimpinellifolium LA2093 </li></ul></ul><ul><ul><ul><li>F2 map </li></ul></ul></ul><ul><ul><ul><li>RIL map </li></ul></ul></ul><ul><ul><li>S. lycopersicum X S. pimpinellifolium PSPL153 </li></ul></ul><ul><ul><ul><li>F2 map </li></ul></ul></ul>
  13. 13. Goal 4 Identify genes or QTLs for traits of interest <ul><li>Identified major genes for: </li></ul><ul><ul><li>LB resistance </li></ul></ul><ul><li>Identified QTLs for many traits, including: </li></ul><ul><ul><li>EB resistance </li></ul></ul><ul><ul><li>Salt tolerance </li></ul></ul><ul><ul><li>Cold tolerance </li></ul></ul><ul><ul><li>Drought tolerance </li></ul></ul><ul><ul><li>LYC content </li></ul></ul>
  14. 14. TG185 (14.9) CT172 (5.0) CT118 (2.0) TG318 (3.9) CT202 (2.0) XLRR.370 (7.1) RLRR.220 (6.0) PK12.340 (27.9) CT93 (23.9) CT242 (11.1) TG441 (13.1) CEL5 (10.7) CT101 (0.0) Chr1 Chr5 Chr9 Chr2 TG507 (1.5) TG250 (0.9) TG620 (0.9) CT59 (36.3) TG337 (36.2) TG484 (22.1) A35.9 (0.0) A35.7 (18.9) TG582 (6.5) CT176 (5.3) CT205 (6.3) TG608 (3.6) XLRR.380 (3.8) CLRR.490 (1.4) PK12.1200 (0.9) PK12.600 (0.0) Chr10 Chr3 TG214 (13.1) TG411 (26.9) XLRR.140 (8.4) TG246 (6.0) TG515 (3.1) TG388 (1.4) CT82 (2.1) S23.140 (3.5) TG621 (4.9) TG66 (3.8) A44 (8.5) TG525 (2.3) CT22 (19.1) CT141 (21.3) TG132 (0.0) TG517 (2.2) TG114 (0.0) Chr7 TG128 (21.1) C21 (31.9) CT52 (11.2) CT135 (2.8) TG61 (10.1) PK12.300 (9.6) TG174 (4.0) TG183 (12.8) CT226 (15.2) TG216 (17.6) TG499 (0.0) Chr11 Chr4 TG163 (4.3) CEL68 (4.3) CT173 (5.4) CT73 (6.0) CT50 (13.7) TG65 (15.0) TG652 (3.8) CT157 (5.3) PK34.300 (2.6) PK34.150 (8.9) TG586 (8.0) TG483 (0.0) Chr8 CT265 (25.0) CT68 (4.1) TG294 (22.4) TG330 (20.9) TG302 (6.4) TG349 (10.4) CT92 (14.7) TG176 (23.6) CD40 (0.0) Chr12 7.0 3.7 4.4 2.0 1.8 5.4 TG393 (26.7) TG36 (14.4) CT107c (3.7) TG546 (8.4) CT107a (5.7) CD17 (9.4) CT55 (3.6) A41.3 (3.6) TG147 (9.2) CT182 (9.2) TG523 (7.0) TG651 (5.8) TG508 (12.0) CT168 (0.0) TG429 (29.1) TG424 (5.6) TG421 (10.0) CT74 (8.1) TG248 (9.3) S11.210 (5.6) TG390 (7.1) TG551 (17.8) TG291 (12.5) XLRR.360 (2.1) CLRR.950 (5.6) RLRR.130 (0.0) RLRR.140 (16.7) TG254 (6.1) TG9 (1.6) C11 (6.4) CT143 (0.0) 8.2 6.8 3.8 5.3 KJB4 (14.4) CT276 (8.1) TG473 (26.3) PK12.320 (10.4) TG565 (0.8) TG283 (0.7) TG180 (3.7) AN23.390 (11.1) CT211 (6.3) CT79 (3.8) TG68 (16.2) CT100 (12.3) TG111 (16.9) CT156 (0.0) TG63 (16.4) TG403 (0.7) TG241 (6.3) CEL58 (3.6) CEL79 (5.9) CT57 (4.7) KJB3 (0.0) CC33 (10.4) CD34 (1.1) TG15 (7.0) CT287a (3.5) CT274 (0.7) TG566 (3.0) CT234 (7.7) TG230 (0.0) CT137 (15.3) TG208a (8.2) TG559 (15.6) TG460 (14.8) TG273 (16.9) TG223 (2.2) TG208b (4.4) TG70 (9.1) XLRR.390 (0.0) AN23.230 (0.0) AN23.210 (4.5) TG24 (4.8) CT197 (0.0) 2.9 Chr6 2.2 EBR1.1 EBR2.1 EBR3.1 EBR5.1 EBR5.2 EBR8.1 EBR8.2 EBR12.1 EBR12.2 EBR11.1 EBR11.2 EBR10.1 EBR9.2 EBR9.1 2.4 EB Resistance QTLs TG314 (20.7) CT107b (8.0) TG279 (8.5) C54 (7.5) TG253 (9.4) TG365 (10.2) CG43 (8.8) CT58 (6.6) TG590 (6.2) AN23.410 (4.7) TG232 (5.0) CT83 (13.6) CT285 (0.0)
  15. 15. <ul><li>Types of tomato developed: </li></ul><ul><ul><li>Processing tomatoes </li></ul></ul><ul><ul><li>Fresh-market tomatoes </li></ul></ul><ul><ul><ul><li>Cherry </li></ul></ul></ul><ul><ul><ul><li>Grape </li></ul></ul></ul><ul><ul><ul><li>Plum </li></ul></ul></ul><ul><ul><ul><li>Medium/large round </li></ul></ul></ul>Goal 5 Develop Tomato Breeding Lines and Cultivars
  16. 16. Goal 5 Develop Tomato Breeding Lines and Cultivars <ul><li>Breeding techniques used: </li></ul><ul><ul><li>Conventional breeding </li></ul></ul><ul><ul><li>Marker-assisted breeding </li></ul></ul>
  17. 17. Tomato Crossing Blocks in the GH
  18. 18. Seedling Preparation in the GH
  19. 19. Plants Growing in the Field
  20. 20. Goal 5 Develop Tomato Breeding Lines and Cultivars <ul><li>Major traits incorporated: </li></ul><ul><ul><li>EB resistance </li></ul></ul><ul><ul><li>LB resistance </li></ul></ul><ul><ul><li>High fruit lycopene content </li></ul></ul><ul><ul><li>High yield </li></ul></ul>
  21. 21. EB-Resistant Breeding Lines <ul><ul><li>Fresh-Market Tomatoes </li></ul></ul><ul><ul><ul><li>Cherry : Available </li></ul></ul></ul><ul><ul><ul><li>Grape : Available </li></ul></ul></ul><ul><ul><ul><li>Med-Large Round : Available </li></ul></ul></ul><ul><ul><li>Processing Tomatoes : Available </li></ul></ul>
  22. 22. High-Lycopene Breeding Lines <ul><ul><li>Fresh-Market Tomatoes </li></ul></ul><ul><ul><ul><li>Cherry : Available </li></ul></ul></ul><ul><ul><ul><li>Grape : Available </li></ul></ul></ul><ul><ul><ul><li>Plum : Available </li></ul></ul></ul><ul><ul><ul><li>Med-Large Round : Available </li></ul></ul></ul><ul><ul><li>Processing Tomatoes : Available </li></ul></ul>
  23. 23. LB-Resistant Breeding Lines <ul><ul><li>Fresh-Market Tomatoes </li></ul></ul><ul><ul><ul><li>Ph-2 + Ph-3 : In the pipeline </li></ul></ul></ul><ul><ul><ul><li>Ph-5 : In the pipeline </li></ul></ul></ul><ul><ul><li>Processing Tomatoes </li></ul></ul><ul><ul><ul><li>Ph-3 : In the pipeline </li></ul></ul></ul><ul><ul><ul><li>Ph-5 : In the pipeline </li></ul></ul></ul>
  24. 24. Penn State EB Resistant Tomatoes
  25. 25. Penn State High LYC Cherry and Grape Tomatoes Grape Cherry Others
  26. 26. Penn State Grape Tomato
  27. 27. Penn State FM Plum Tomato
  28. 28. Penn State Fresh Market Tomato
  29. 29. Penn State Processing Tomato
  30. 30. Penn State Hi-yielding Processing Tomatoes
  • UsmanAhmedShah1

    Sep. 26, 2019
  • MAbedi1

    Aug. 31, 2017
  • DrAyadWaleed

    Feb. 13, 2014
  • subalsarker

    Dec. 11, 2013

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