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Micronucleus and H2AX assay

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In response to clastogen DNA double strand breaks, H2AX is phosphorylated within minutes at Ser 139, and is then referred as γH2AX. Contrary to γH2AX, micronuclei are a late marker of damages either to DNA and be related to aneugens nor clastogens effect. By detecting both H2AX and micronuclei on our high content screening plateform, our assay provide a efficient tool to assess the genotoxicity properties of your compounds.

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Micronucleus and H2AX assay

  1. 1. In response to clastogen DNA double strand breaks, H2AX is phosphorylated within minutes at Ser 139, and is then referred as γH2AX. Contrary to γH2AX, micronuclei are a late marker of damages either to DNA and be related to aneugens nor clastogens effect. By detecting both H2AX and micronuclei on our high content screening plateform, our assay provide a efficient tool to assess the genotoxicity properties of your compounds. G E N O T O X I C I T Y Study DNA integrity Example of Benzo[a]pyrene Cells: - adherent cells - primary cells or cell line - with or without S9 Compounds: powder or in stock solution HepG2 cells exposed 24 hours to several concentrations of Benzo[a]pyrene. Plates : 96 or 384 wells plates Requirements Micronucleus and H2AX assay 96 or 384-well plates CTRL B[A]P 100µM Micronucleus Hoechst, H2AX Micronucleus H2AX H2AX For more information, go to www.hcs-pharma.com For a quotation, contact us at welcome@hcs-pharma.com Ask for our short report on HepG2 cells exposed to our in house genotoxicity library ! Detection of micronuclei Detection of H2AX spots

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