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Automated Comet assay

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The comet assay is a sensitive, well established technique for quantifying DNA damage in eukaryotic cells. Compatible with the detection of a wide range of DNA damaging agents, its principle consists in migration of fragmented DNA in an electrophoresis gel (damaged DNA forming the tail of the comet), while intact DNA moves at a slower rate (head of the comet). The percentage of fragmented DNA in the comet tail is a direct measure of DNA damage.

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Automated Comet assay

  1. 1. The comet assay is a sensitive, well established technique for quantifying DNA damage in eukaryotic cells. Compatible with the detection of a wide range of DNA damaging agents, its principle consists in migration of fragmented DNA in an electrophoresis gel (damaged DNA forming the tail of the comet), while intact DNA moves at a slower rate (head of the comet). The percentage of fragmented DNA in the comet tail is a direct measure of DNA damage. Automated Comet assay CTRL Etoposide 500µM Study DNA integrity Example of Etoposide For more information, go to www.hcs-pharma.com For a quotation, contact us at welcome@hcs-pharma.com Comet slide Ask for our short report on HepG2 cells exposed to our in house genotoxicity library ! Cells: - suspension or adherent - primary cells or cell line - with or without S9 Compounds: powder or in stock solution HepG2 cells exposed 3 hours to several concentrations of Etoposide. Trevigen automated 96 wells comet assay Requirements SegmentationSegmentation DNA DNA G E N O T O X I C I T Y

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