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Polymerase chain reaction

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PCR

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Polymerase chain reaction

  1. 1. POLYMERASE CHAIN REACTION BY: AIMEN HAIDER IRHA JAMSHED
  2. 2. INTRODUCTION DEFINITION “ It is a test tube method for amplifying a selected DNA sequence that does not rely on the biological cloning method.”
  3. 3. STEPS OF A POLYMERASE CHAIN REACTION  Primer construction  Denature the DNA  Annealing of primers to single-stranded DNA  Chain extension
  4. 4. ADVANTAGES OF PCR Advantage over cloning – sensitivity and speed Sensitivity : DNA sequence present in an individual cell ; scarce amounts can be studied. Speed : DNA is very fast and technically less difficult than traditional cloning methods using recombinant DNA techniques.
  5. 5. APPLICATIONS PCR has become a very common tool for a large number of applications 1)Comparison of a normal gene with a mutant form of the gene. 2)Detection of low-abundance nucleic acid sequences. 3)Forensic analysis of DNA samples. 4)Prenatal diagnosis and carrier detection of cystic fibrosis.
  6. 6. Multiplex PCR Definition : “ The simultaneous amplification of multiple regions of a target DNA using multiple primer pairs is known as multiplex PCR. It allows detection of the loss of 1 or more exons in a gene with many exons.” Example : gene for CFTR which has 27 genes

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