PHASE CONTRAST  MICROSCOPY
<ul><li>Phase contrast microscope is a technique which makes highly transparent objects more visible without killing them....
PRINCIPLE <ul><li>It converts the different degrees of retardation of light rays passing through a translucent material in...
COMPONENTS <ul><li>Light source </li></ul><ul><li>Annular diaphragm </li></ul><ul><li>Condenser lens </li></ul><ul><li>Spe...
 
 
 
 
Cheek cell
Clostridium botulinum,  the bacterium responsible for botulism, with subterminal oval endospores;
Spirillum volutans,  a very large bacterium with flagellar bundles
Paramecium stained to show a large central macronucleus with a small spherical micronucleus at its side
APPLICATIONS <ul><li>Objects with poor intensity can be resolved well. </li></ul><ul><li>Image of the unstained object can...
THANK YOU HARIS P MSC BIOTECHNOLOGY MA COLLEGE  ERNAKULAM
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Phase contrst microscope.HRS

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Phase contrst microscope.HRS

  1. 2. PHASE CONTRAST MICROSCOPY
  2. 3. <ul><li>Phase contrast microscope is a technique which makes highly transparent objects more visible without killing them. </li></ul><ul><li>Invented by the Dutch physicist Frederick </li></ul><ul><li>Zernike in 1930 </li></ul>
  3. 4. PRINCIPLE <ul><li>It converts the different degrees of retardation of light rays passing through a translucent material into amplitude differences to create an image with increased contrast. </li></ul>
  4. 5. COMPONENTS <ul><li>Light source </li></ul><ul><li>Annular diaphragm </li></ul><ul><li>Condenser lens </li></ul><ul><li>Specimen stage </li></ul><ul><li>Objective lens </li></ul><ul><li>Phase plate </li></ul><ul><li>Telescopic lens </li></ul><ul><li>Eye piece </li></ul>
  5. 10. Cheek cell
  6. 11. Clostridium botulinum, the bacterium responsible for botulism, with subterminal oval endospores;
  7. 12. Spirillum volutans, a very large bacterium with flagellar bundles
  8. 13. Paramecium stained to show a large central macronucleus with a small spherical micronucleus at its side
  9. 14. APPLICATIONS <ul><li>Objects with poor intensity can be resolved well. </li></ul><ul><li>Image of the unstained object can be obtained </li></ul><ul><li>It is widely used in microbiology and tissue culture research to detect bacteria, cellular organelles and other small entities in living specimens </li></ul><ul><li>It allows the visualization of living unstained cells and their organelles. </li></ul><ul><li>For the detection of bacterial components such as endospores and inclusion bodies containing poly β hydroxy butyrate, polymetaphosphate, sulpher and other substances </li></ul>
  10. 15. THANK YOU HARIS P MSC BIOTECHNOLOGY MA COLLEGE ERNAKULAM

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