Assisted Embryo Hatching
• Assisted hatching is an IVF technique in which the zona
is treated prior to embryo transfer in ...
Indications for Assisted Hatching:-
•Elevated maternal age
•Elevated follicle stimulating hormone [FSH] level
•Evidence of...
Embryo hatching with laser Blastocyst
hatching in preparation  human embryo at
37 days
 
Advantages of assisted embryo hatching
Assisted hatching has demonstrated the potential for improving
embryo implantation ...
Techniques of Assisted Embryo Hatching
• Mechanical Hatching
• Chemical Hatching
• Laser Hatching
Mechanical hatching
• In this the embryo cover is slit open mechanically with the help of
a thin long drawn out glass need...
Mechanical technique: partial zona
dissection
Chemical hatching
• Creating a hole in the zona pellucida of an embryo by using
acidic tyrode’s solution .
Procedure:
• Th...
Acidic tyrode’s solution of pronase is used for
drilling holes in zona pellucida.
Laser assisted Hatching
• This technique involves the creation of a precise gap in the zona
pellucida of selected embryos ...
Procedure:
• It allows direct delivery of laser beam through the objectives.
• 1.48 micron diode laser is advantageous ove...
Assisted hatching done by using laser light
The embryo is held
with gentle suction
using a glass pipette
The red “pilot li...
Importance of Laser Assisted Hatching
• This specifically designed laser system includes the laser,
which serves as the en...
If required this facility
Please contact us:
Dr. Rama's Institute for Fertility
6-3-788/33, Opp. Chandana Brothers,Ameerpe...
Hatching
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Hatching

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Hatching

  1. 1. Assisted Embryo Hatching • Assisted hatching is an IVF technique in which the zona is treated prior to embryo transfer in order to weaken the wall of the embryo and thus improve the likelihood of successful hatching and embryo implantation. • Just prior to embryo transfer, the developing embryo must"hatch” out of its outer shell (zona pellucida). • Some embryos seem to have a thicker shell that may decrease their ability to implant • In such cases Advance technique of IVF with assisted hatching is performed.
  2. 2. Indications for Assisted Hatching:- •Elevated maternal age •Elevated follicle stimulating hormone [FSH] level •Evidence of a thickened zone pellucida of the embryos in IVF •Unexplained implantation failure after two or more embryo transfers. •Frozen thowed embryo. •Excessive fragmentation >20%
  3. 3. Embryo hatching with laser Blastocyst hatching in preparation  human embryo at 37 days  
  4. 4. Advantages of assisted embryo hatching Assisted hatching has demonstrated the potential for improving embryo implantation rates and clinical pregnancy rates in select patient groups undergoing IVF. - Fewer embryos required for transfer. - Increased implantation success rates - Allow for blastocyst culture.
  5. 5. Techniques of Assisted Embryo Hatching • Mechanical Hatching • Chemical Hatching • Laser Hatching
  6. 6. Mechanical hatching • In this the embryo cover is slit open mechanically with the help of a thin long drawn out glass needle. • This is done with the help of a machine called micromanipulator Procedure: • The zona pellucida is pierced with a very thin glass micro needle through both sides, the needle tip being controlled in the perivilelline space by eye. • Then the suction of the holding pipette is stopped and the holding pipette is rubbed against the trapped area of the zona until this area has been completely abraded.
  7. 7. Mechanical technique: partial zona dissection
  8. 8. Chemical hatching • Creating a hole in the zona pellucida of an embryo by using acidic tyrode’s solution . Procedure: • The fine micropipette containing acidic tyrode’s solution is brought very close to the zona pellucida and the acidic solution expelled gently over a small area (~20-30mm) until the zona is dissolved through to the inside. • After the procedure the embryos are thoroughly washed in fresh medium and cultured until the time of transfer.
  9. 9. Acidic tyrode’s solution of pronase is used for drilling holes in zona pellucida.
  10. 10. Laser assisted Hatching • This technique involves the creation of a precise gap in the zona pellucida of selected embryos using a 1.48 micron infrared diode laser. Contact laser / Non contact laser • Contact laser: laser radiation has to be delivered directly to the zona called contact laser • Non contact laser: laser gamete manipulation has described in the non-contact mode. Using various wavelengths.
  11. 11. Procedure: • It allows direct delivery of laser beam through the objectives. • 1.48 micron diode laser is advantageous over all other techniques as at 1.48 wave length , radiation is non mutagenic • The radiation is administered in the non contact mode without embryo micromanipulation.
  12. 12. Assisted hatching done by using laser light The embryo is held with gentle suction using a glass pipette The red “pilot light “ provides a visual target for the laser. The white circle marks the safety region based on temperature. The laser is usually fired 3 times to produce a small hole in the zone
  13. 13. Importance of Laser Assisted Hatching • This specifically designed laser system includes the laser, which serves as the energy source to create an opening in the zona pellucida. • A computer which allows the operator to precisely control the laser energy output, laser pulse duration and gap size. • Takes less time and does not expose the embryos to potentially adverse chemicals. • This procedure appears to be quick, precise and is chemical free.
  14. 14. If required this facility Please contact us: Dr. Rama's Institute for Fertility 6-3-788/33, Opp. Chandana Brothers,Ameerpet, Hyderabad - 16, Andhra Pradesh, India. Phones: +91-040- 23401135, 23402294. Email: ramafertility@yahoo.com info@fertilityindia.com

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