Introduction Oil Palm Cultivation• Oil Palm is a tropical perennial monocot species in the order Arecales of the family Aracaceae.• The African Oil Palm, Elaeis guineensis is native to West Africa, occurring between Angola and Gambia, while the American Oil Palm Elaeis oleifera is native to tropical Central America and South America. (Dransﬁeld et al., 2005)• Oil palm was first brought to Malaya in early twentieth century as ornamental plant. It is now one of the main commodity crop in Malaysia.
Vitamin E in Oil Palm• Vitamin E consists of tocopherols and tocotrienols .• Vitamin E in palm oil is made up of 70% tocotrienol and 30% of tocopherol.• Palm oil is nature’s richest source of tocotrienols.• Whereas most other vegetable oils consists of only tocopherols. (Koh, 2003)
Main ObjectivesTo isolate full length cDNA of gama-tocopherol methyltransferase (γ-TMT)gene from oil palm. To obtain γ-TMT gene full gene structure DNA sequence. To identify single nucleotide polymorphism (SNP) in γ-TMT gene.
Literature Review Vitamin E Chemical Structure Tocopherol Tocotrienol• Tocopherols and tocotrienols consists of a chromanol head group with 1,2 or 3 methyl groups and an isoprenoid side chain. (Chen et al., 2006).• Tocopherols have a saturated phytyl tail, while tocotrienols have a 3-fold unsaturated side chain. (DellaPenna & Pagson,2005).• Tocopherols and tocotrienols differ only in the number and position of methyl substituents on the aromatic ring with α- having three, β- and γ- having two, and δ- tocopherol having only one. (Hunter & Cahoon, 2007).