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Veena et al.
Int J Curr Pharm Res, Vol 9, Issue 5, 152-154
152
PHARMACOLOGICAL EVALUATION OF ANTIANXIETY ACTIVITY OF DESMOSTACHYA
BIPINNATA LEAVES IN ANIMAL MODELS
Original Article
VEENA RANI I.*1, KIRANMAI G.1, RAVI PRATAP PULLA2
1Department of Pharmacology, SSJ College of Pharmacy, Vattinagulapally, Hyderabad-75, 2
Received: 22 May 2017, Revised and Accepted: 22 Jul 2017
Department of PA and QA, SSJ College of
Pharmacy, Vattinagulapally, Hyderabad-75
Email: vina913@gmail.com
Objective: Anxiety is a widespread psychiatric disorder affecting around 5% of the population. Furthermore, it is difficult to predict patient’s
response to any given treatment. In the traditional systems of medicine, many plants have been used to treat anxiety and depression for thousands
of years. Desmostachyabipinnata belongs to the family Poaceae, have pharmacological actions like
ABSTRACT
dysentery and menorrhagia, and as a diuretic. The
present study was designed to evaluate the antianxiety activity of the alcoholic and aqueous extracts of Desmostachyabipinnata leaves in rodents.
Methods: Antianxiety activity was screened by different methods like elevated plus maze model and actophotometer.
Results: The results infer that reduced aversion fear elicits anti-anxiety activity.
Conclusion: It was concluded that alcoholic and aqueous extracts of Desmostachyabipinnata leaves are having anti-anxiety activity among which
alcoholic extract of Desmostachyabipinnata leaves showing more significant activity over the aqueous extract.
Keywords: Desmostachyabipinnata, Antianxiety activity, Elevated plus maze, Actophotometer
© 2017 The Authors. Published by Innovare Academic Sciences Pvt Ltd. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/)
DOI: http://dx.doi.org/10.22159/ijcpr.2017v9i5.22159
INTRODUCTION
According to the World Health Organization nearly 450 million
people suffering from psychological or behavioural ailments, yet
only a small minority of them receive even the most elementary
treatment. This amounts to 12.3% of the global load of disease and
will rise to 15% by 2020 [1]. In the search for new therapeutic
products for the treatment of neurological disorders, medicinal plant
research has progressed constantly representing the
pharmacological effectiveness of diverse plant species in a variety of
animal models [2]. It is now clear that without awareness of clinical
and biological aspects of anxiety and depression, it is difficult to
offer actual treatment approaches for the patients. There has been
an intensive study of a variety of neurobiological aspects of
depression and anxiety from the past decades.
Presently the most commonly approved medications for anxiety
disorders are benzodiazepines but their clinical applications as
antianxiety agents are limited due to their undesirable effects.
Therefore the development of new pharmacological agents from
plant sources are well justified.
Desmostachyabipinnata, commonly known in english as half a
grass, big cordgrass, and salt reed-grass is an old world perennial
grass, used in human history. In India, it is known
as Daabh,
MATERIALS AND METHODS
Darbha, Kusha. From literature survey it was found that
Desmostachyabipinnta processes antibacterial activity [3] anti-
ulcerogenic [4], antioxidant and DNA damage protection activity
[5], anti-histaminic activity [6], anti-obesity activity [7], glycemic
Status in Non-diabetic Rats [8], diuretic and laxative activity [9],
anti-diarrheal activity [10], anti urolithiatic activity [11], anti-
helicobacter activity [12], use in gut disorders and asthma [13],
analgesic and anti-inflammatory [14], hepatoprotective activity
[15, 16]. The purpose of the present study was to evaluate the
antianxiety activity of alcoholic and aqueous extracts of
Desmostachyabipinnata. In spite of extensive literature available
on some components of this plant, there is no known data
regarding the pharmacological evaluation on anti-anxiety activity.
Thus, this study was intended to performanti-anxiety in
experimental animal models.
Animals
Wistar rats (180±20g) of either sex were procured from institutional
animal house and they were retained in the groups of six under the
standard laboratory conditions (Temp 23±2 °C, relative humidity
50-60% and 12:12 h light–dark cycle), with standard pellet diet
(Amrut brand) and water ad libitum. Experiments were performed
only after the animals had acclimated to the laboratory conditions
for at least seven days. The experimental protocol was approved by
institutional animal ethics committee (1757/PO/RcBiBt/CPCSEA).
Plant material collection and extraction
The leaves of Desmostachya Bipinnata were collected cleaned,
reduced to small fragments, air dried under shade at room
temperature and coarsely powdered in a mixer. The powdered
material was taken up for alcoholic extraction by taking 20 gms of
powdered material into 250 ml beaker containing 200 ml of alcohol.
The contents were mixed well and boiled up to 50-60 °C for 4-5 h.
Further, the extract was filtered with whatmann filter paper. The
filtrate was boiled until the concentrated residue is formed.
The aqueous extract was prepared by taking 20 gms of finely cut
leaves into 250 ml beaker containing 200 ml of water and was boiled
up to 80-100 °C for 4-5 h. Further, the extract was filtered with
whatmann filter paper. The filtrate was boiled until the concentrated
residue is formed. This residue was used for the experiment.
The aqueous and alcoholic extracts of Desmostachyabipinnata
suspended in water in presence of 3%v/v Tween-80 solution. The
drugs were administered orally for investigation a determination.
Each time preparations of the extracts were prepared when
required. The drugs were administered at a constant volume of 10
ml/kg for each animal. The aqueous and alcoholic extracts of
Desmostachyabipinnata leaves were tested for anti-anxiety activity
using elevated plus maze and act ophotometer.
Treatment schedule
Animals were divided into four (I-VI) groups.
International Journal of Current Pharmaceutical Research
ISSN- 0975-7066 Vol 9, Issue 5, 2017
Veena et al.
Int J Curr Pharm Res, Vol 9, Issue 5, 152-154
153
Group I-Control group received distilled water (1 ml, p. o).
Group II-Standard group received Diazepam (10 mg/kg i. p).
Group III (AQEDB-1)-Test group received an aqueous extract of
Desmostachyabipinnata (200 mg/kg p. o).
Group IV (AQEDB-2)-Test group received aqueous extract of
Desmostachyabipinnata (400 mg/kg p. o).
Group V (ALEDB-1)-Test group received an alcoholic extract of
Desmostachyabipinnata (200 mg/kg p. o).
Group VI (ALEDB-2)-Test group received an alcoholic extract of
Desmostachyabipinnata (400 mg/kg p. o).
Anti-anxiety activity by elevated plus maze (EPM) model
The apparatus comprises of two open (35x5 cm) and two closed
arms (30x5x15 cm) that extend from a common central platform
(5x5 cm). The entire maze is elevated to a height of 50 cm above the
ground level. Rats weighing (150–200 gms) were housed in a pair of
10 d prior to the test in the apparatus and were handled by the
investigator on alternate days to reduce stress. 30and 60 min
following oral administration of the drugs, each rat was located in
the centre of the maze which is facing enclosed arms. During 5 min
session, a number of entries into open arm and time spent in it was
noted. The procedure was conducted preferably in a sound
attenuated environment.
Anti-anxiety activity by Locomotor activity
The locomotor activity can be easily studied with the help of
actophotometer [17], after the drug treatments rats are placed
individually in the activity cage for 10 min. Note the basal activity
score of all the animals. After 30 min re-test each rat for activity
scores for 10 min. Note the difference in the activity, before and after
drug treatment. Calculate percent decrease in motor activity.
Statistical analysis
The values were expressed as mean±SEM and data were analyzed
using one-way ANOVA followed by T-test. Two sets of comparison had
made. i.e. Normal control Vs All treated groups. Differences between
groups were considered significant at P<0.001 and P<0.05 levels.
Table 1: Data obtained from elevated plus maze experiment
Groups Dose No of entries Time spent in time spent in
Open Arm Closed Arm Open Arm Closed Arm
Control (Distilled Water) ------ 2.36±1.25 2.48.10±1.58
* 30.12±8.05
* 266.45±21.47
* *
Standard (Diazepam) 10 mg/kg 4.21±0.65 4.01±1.45 26.88±12.36 170.55±30.54
AQEDB-1 200 mg/kg 3.95±0.87 3.10±1.44
** 30.54±11.51
** 150.20±22.36
**
AQEDB-2
*
400 mg/kg 3.50±1.54 3.14±0.24
* 28.23±1.65
* 165.41±19.32
*
ALEDB-1
*
200 mg/kg 3.52±1.87 2.98±1.72
** 32.14±1.74
** 162.22±24.65
**
ALEDB-2
**
400 mg/kg 3.02±.44 2.71±1.05
** 25.21±0.89
** 154.36±32.12
** **
Values expressed mean±SEM ***p<0.001, **p<0.01, *
RESULTS
p<0.05 when compared to Standard group. One-way ANOVA followed by Dunnett’s test
Elevated plus maze (EPM) model
From the experiment, it was observed that rats given aqueous and
alcohol soluble fraction at a dose of 200 mg/kg and 400 mg/kg
body weight, stayed more time in open arm of Elevated plus Maze
apparatus in comparison to standard and control group. Moreover,
they also stayed less time in closed arm of Elevated plus Maze
apparatus in comparison to standard and control group. The value
obtained from these fraction were statistically significant
(p<0.05).
Actophotometer model
Anxiolytic property of aqueous and alcoholic extracts of
Desmostacyabipinnata was studied at a dose of 200 and 400 mg/Kg,
using Actophotometer model.
The percentage of reduction in locomotor activity with diazepam (10
mg/kg i. p) after 1 hour is 80 % i.e. there is significant (P<0.001)
decrease in locomotor activity compare to control, where as dose of
AQEDB and ALEDB (200 and 400 mg/kg i. p) showed dose dependent
decrease in locomotor activity that is 78.3% and 75.8% respectively
when compared to standard. The values are highly significant (P<0.001)
Table 2: Effect of extracts of Desmostachyabipinnata on Locomotor activity
S. No. Groups Dose(mg/kg) Locomotor activity in 10 min
Before After % change in activity
1 Control - 245.12±35.44 -- ---
2 Standard 30 270.32±45.02 82.33±10.69 68.5
3 AQEDB-1 200 365.21±43.65 79.06±9.26
** 78.3
**
4 AQEDB-2 400 374.11±36.54 70.65±8.55
*** 81.2
***
5 ALEDB-1 200 286.60±36.88 69.05±9.88
** 75.8
**
6 ALEDB-2 400 300.33±39.65 60.65±6.54
*** 80
***
Values expressed mean±SEM ***p<0.001, **p<0.01, *p<0.05 when compared to standard group. One-way ANOVA followed by Dunnett’s test, The
results are expressed as means±SEM Differences in mean values between groups were analyzed by a one-way analysis of variance (ANOVA).
Statistical significance was assessed as p<0.05.
DISCUSSION
The anti-anxiety activity of Desmostachyabipinnata was evaluated by
using a widely used model like elevated plus-maze. Mean a number of
entries and time spent by rats in open arms amongst aqueous and
alcoholic extracts of Desmostachyabipinnata significantly increased
mean number of entries and mean time spent by rats in open arms of
elevated plus maze apparatus at the dose of 200 mg/kg with respect to
control, thereby showing anti-anxiety activity. Desmostachyabipinnata
contains different chemical constituents like alkaloids, Carbohydrates,
Saponins, Tannins, Phytosterols and Phenolic compounds, lignin,
flavonoids, protein and free amino acids [18].
It was earlier reported that several essential oils that are obtained
from florae are engaged to balance emotions, develop physical and
mental well-being [19] by different mechanisms. Therefore,
essential oils that are present in the extracts of Desmostachya
bipinnata [20] may be responsible for the anti-anxiety activity.
Veena et al.
Int J Curr Pharm Res, Vol 9, Issue 5, 152-154
154
Further, the anxiolytic effect of flavonoids has been attributed to its
effect on the central nervous system and benzodiazepine receptors
[21]. It may be possible that the mechanism of anxiolytic action of
AQEDB and ALEDB could be due to the binding of any of these
phytochemicals to the GABAA-BZD complex. So the anxiolytic
activity of AQEDB and ALEDB might involve an action on GABA ergic
transmission or effects on serotonergic transmission or due to its
mixed aminergic potentiating effect.
Spontaneous locomotor activity is considered as an index of
alertness and can be helpful to confirm the general depressive
activity of any drug. The reduction in motor activity indicates the
level of CNS excitability and this may be related to sedation due to
CNS depression. However, in the present study, the AQEDB and
ALEDB was found to have decreased effect on the locomomotor
activity in actophotometer.
CONCLUSION
The results obtained in this study indicate that the alcoholic and
aqueous extracts fractions of the leaves of Desmostacyabipinnata
have significant CNS Depressant and Anxiolytic activities in animal
model systems. The medicinal values of the plant leaves may be
related to their constituent phytochemicals. So, further detailed
investigations are needed to isolate and identify the active
compounds present in the plant extract and its various fractions. It
will help in the development of novel and safe drugs for the
treatment of different types of CNS disorders.
CONFLICT OF INTERESTS
Declare none
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How to cite this article
• Veena Rani I, Kiranmai G, Ravi Pratap Pulla. Pharmacological
evaluation of antianxiety activity of Desmostachya bipinnata leaves
in animal models. Int J Curr Pharm Res 2017;9(5):152-154.

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Pharmacological_Evaluation_of_Antianxiet (1).pdf

  • 1. Veena et al. Int J Curr Pharm Res, Vol 9, Issue 5, 152-154 152 PHARMACOLOGICAL EVALUATION OF ANTIANXIETY ACTIVITY OF DESMOSTACHYA BIPINNATA LEAVES IN ANIMAL MODELS Original Article VEENA RANI I.*1, KIRANMAI G.1, RAVI PRATAP PULLA2 1Department of Pharmacology, SSJ College of Pharmacy, Vattinagulapally, Hyderabad-75, 2 Received: 22 May 2017, Revised and Accepted: 22 Jul 2017 Department of PA and QA, SSJ College of Pharmacy, Vattinagulapally, Hyderabad-75 Email: vina913@gmail.com Objective: Anxiety is a widespread psychiatric disorder affecting around 5% of the population. Furthermore, it is difficult to predict patient’s response to any given treatment. In the traditional systems of medicine, many plants have been used to treat anxiety and depression for thousands of years. Desmostachyabipinnata belongs to the family Poaceae, have pharmacological actions like ABSTRACT dysentery and menorrhagia, and as a diuretic. The present study was designed to evaluate the antianxiety activity of the alcoholic and aqueous extracts of Desmostachyabipinnata leaves in rodents. Methods: Antianxiety activity was screened by different methods like elevated plus maze model and actophotometer. Results: The results infer that reduced aversion fear elicits anti-anxiety activity. Conclusion: It was concluded that alcoholic and aqueous extracts of Desmostachyabipinnata leaves are having anti-anxiety activity among which alcoholic extract of Desmostachyabipinnata leaves showing more significant activity over the aqueous extract. Keywords: Desmostachyabipinnata, Antianxiety activity, Elevated plus maze, Actophotometer © 2017 The Authors. Published by Innovare Academic Sciences Pvt Ltd. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/) DOI: http://dx.doi.org/10.22159/ijcpr.2017v9i5.22159 INTRODUCTION According to the World Health Organization nearly 450 million people suffering from psychological or behavioural ailments, yet only a small minority of them receive even the most elementary treatment. This amounts to 12.3% of the global load of disease and will rise to 15% by 2020 [1]. In the search for new therapeutic products for the treatment of neurological disorders, medicinal plant research has progressed constantly representing the pharmacological effectiveness of diverse plant species in a variety of animal models [2]. It is now clear that without awareness of clinical and biological aspects of anxiety and depression, it is difficult to offer actual treatment approaches for the patients. There has been an intensive study of a variety of neurobiological aspects of depression and anxiety from the past decades. Presently the most commonly approved medications for anxiety disorders are benzodiazepines but their clinical applications as antianxiety agents are limited due to their undesirable effects. Therefore the development of new pharmacological agents from plant sources are well justified. Desmostachyabipinnata, commonly known in english as half a grass, big cordgrass, and salt reed-grass is an old world perennial grass, used in human history. In India, it is known as Daabh, MATERIALS AND METHODS Darbha, Kusha. From literature survey it was found that Desmostachyabipinnta processes antibacterial activity [3] anti- ulcerogenic [4], antioxidant and DNA damage protection activity [5], anti-histaminic activity [6], anti-obesity activity [7], glycemic Status in Non-diabetic Rats [8], diuretic and laxative activity [9], anti-diarrheal activity [10], anti urolithiatic activity [11], anti- helicobacter activity [12], use in gut disorders and asthma [13], analgesic and anti-inflammatory [14], hepatoprotective activity [15, 16]. The purpose of the present study was to evaluate the antianxiety activity of alcoholic and aqueous extracts of Desmostachyabipinnata. In spite of extensive literature available on some components of this plant, there is no known data regarding the pharmacological evaluation on anti-anxiety activity. Thus, this study was intended to performanti-anxiety in experimental animal models. Animals Wistar rats (180±20g) of either sex were procured from institutional animal house and they were retained in the groups of six under the standard laboratory conditions (Temp 23±2 °C, relative humidity 50-60% and 12:12 h light–dark cycle), with standard pellet diet (Amrut brand) and water ad libitum. Experiments were performed only after the animals had acclimated to the laboratory conditions for at least seven days. The experimental protocol was approved by institutional animal ethics committee (1757/PO/RcBiBt/CPCSEA). Plant material collection and extraction The leaves of Desmostachya Bipinnata were collected cleaned, reduced to small fragments, air dried under shade at room temperature and coarsely powdered in a mixer. The powdered material was taken up for alcoholic extraction by taking 20 gms of powdered material into 250 ml beaker containing 200 ml of alcohol. The contents were mixed well and boiled up to 50-60 °C for 4-5 h. Further, the extract was filtered with whatmann filter paper. The filtrate was boiled until the concentrated residue is formed. The aqueous extract was prepared by taking 20 gms of finely cut leaves into 250 ml beaker containing 200 ml of water and was boiled up to 80-100 °C for 4-5 h. Further, the extract was filtered with whatmann filter paper. The filtrate was boiled until the concentrated residue is formed. This residue was used for the experiment. The aqueous and alcoholic extracts of Desmostachyabipinnata suspended in water in presence of 3%v/v Tween-80 solution. The drugs were administered orally for investigation a determination. Each time preparations of the extracts were prepared when required. The drugs were administered at a constant volume of 10 ml/kg for each animal. The aqueous and alcoholic extracts of Desmostachyabipinnata leaves were tested for anti-anxiety activity using elevated plus maze and act ophotometer. Treatment schedule Animals were divided into four (I-VI) groups. International Journal of Current Pharmaceutical Research ISSN- 0975-7066 Vol 9, Issue 5, 2017
  • 2. Veena et al. Int J Curr Pharm Res, Vol 9, Issue 5, 152-154 153 Group I-Control group received distilled water (1 ml, p. o). Group II-Standard group received Diazepam (10 mg/kg i. p). Group III (AQEDB-1)-Test group received an aqueous extract of Desmostachyabipinnata (200 mg/kg p. o). Group IV (AQEDB-2)-Test group received aqueous extract of Desmostachyabipinnata (400 mg/kg p. o). Group V (ALEDB-1)-Test group received an alcoholic extract of Desmostachyabipinnata (200 mg/kg p. o). Group VI (ALEDB-2)-Test group received an alcoholic extract of Desmostachyabipinnata (400 mg/kg p. o). Anti-anxiety activity by elevated plus maze (EPM) model The apparatus comprises of two open (35x5 cm) and two closed arms (30x5x15 cm) that extend from a common central platform (5x5 cm). The entire maze is elevated to a height of 50 cm above the ground level. Rats weighing (150–200 gms) were housed in a pair of 10 d prior to the test in the apparatus and were handled by the investigator on alternate days to reduce stress. 30and 60 min following oral administration of the drugs, each rat was located in the centre of the maze which is facing enclosed arms. During 5 min session, a number of entries into open arm and time spent in it was noted. The procedure was conducted preferably in a sound attenuated environment. Anti-anxiety activity by Locomotor activity The locomotor activity can be easily studied with the help of actophotometer [17], after the drug treatments rats are placed individually in the activity cage for 10 min. Note the basal activity score of all the animals. After 30 min re-test each rat for activity scores for 10 min. Note the difference in the activity, before and after drug treatment. Calculate percent decrease in motor activity. Statistical analysis The values were expressed as mean±SEM and data were analyzed using one-way ANOVA followed by T-test. Two sets of comparison had made. i.e. Normal control Vs All treated groups. Differences between groups were considered significant at P<0.001 and P<0.05 levels. Table 1: Data obtained from elevated plus maze experiment Groups Dose No of entries Time spent in time spent in Open Arm Closed Arm Open Arm Closed Arm Control (Distilled Water) ------ 2.36±1.25 2.48.10±1.58 * 30.12±8.05 * 266.45±21.47 * * Standard (Diazepam) 10 mg/kg 4.21±0.65 4.01±1.45 26.88±12.36 170.55±30.54 AQEDB-1 200 mg/kg 3.95±0.87 3.10±1.44 ** 30.54±11.51 ** 150.20±22.36 ** AQEDB-2 * 400 mg/kg 3.50±1.54 3.14±0.24 * 28.23±1.65 * 165.41±19.32 * ALEDB-1 * 200 mg/kg 3.52±1.87 2.98±1.72 ** 32.14±1.74 ** 162.22±24.65 ** ALEDB-2 ** 400 mg/kg 3.02±.44 2.71±1.05 ** 25.21±0.89 ** 154.36±32.12 ** ** Values expressed mean±SEM ***p<0.001, **p<0.01, * RESULTS p<0.05 when compared to Standard group. One-way ANOVA followed by Dunnett’s test Elevated plus maze (EPM) model From the experiment, it was observed that rats given aqueous and alcohol soluble fraction at a dose of 200 mg/kg and 400 mg/kg body weight, stayed more time in open arm of Elevated plus Maze apparatus in comparison to standard and control group. Moreover, they also stayed less time in closed arm of Elevated plus Maze apparatus in comparison to standard and control group. The value obtained from these fraction were statistically significant (p<0.05). Actophotometer model Anxiolytic property of aqueous and alcoholic extracts of Desmostacyabipinnata was studied at a dose of 200 and 400 mg/Kg, using Actophotometer model. The percentage of reduction in locomotor activity with diazepam (10 mg/kg i. p) after 1 hour is 80 % i.e. there is significant (P<0.001) decrease in locomotor activity compare to control, where as dose of AQEDB and ALEDB (200 and 400 mg/kg i. p) showed dose dependent decrease in locomotor activity that is 78.3% and 75.8% respectively when compared to standard. The values are highly significant (P<0.001) Table 2: Effect of extracts of Desmostachyabipinnata on Locomotor activity S. No. Groups Dose(mg/kg) Locomotor activity in 10 min Before After % change in activity 1 Control - 245.12±35.44 -- --- 2 Standard 30 270.32±45.02 82.33±10.69 68.5 3 AQEDB-1 200 365.21±43.65 79.06±9.26 ** 78.3 ** 4 AQEDB-2 400 374.11±36.54 70.65±8.55 *** 81.2 *** 5 ALEDB-1 200 286.60±36.88 69.05±9.88 ** 75.8 ** 6 ALEDB-2 400 300.33±39.65 60.65±6.54 *** 80 *** Values expressed mean±SEM ***p<0.001, **p<0.01, *p<0.05 when compared to standard group. One-way ANOVA followed by Dunnett’s test, The results are expressed as means±SEM Differences in mean values between groups were analyzed by a one-way analysis of variance (ANOVA). Statistical significance was assessed as p<0.05. DISCUSSION The anti-anxiety activity of Desmostachyabipinnata was evaluated by using a widely used model like elevated plus-maze. Mean a number of entries and time spent by rats in open arms amongst aqueous and alcoholic extracts of Desmostachyabipinnata significantly increased mean number of entries and mean time spent by rats in open arms of elevated plus maze apparatus at the dose of 200 mg/kg with respect to control, thereby showing anti-anxiety activity. Desmostachyabipinnata contains different chemical constituents like alkaloids, Carbohydrates, Saponins, Tannins, Phytosterols and Phenolic compounds, lignin, flavonoids, protein and free amino acids [18]. It was earlier reported that several essential oils that are obtained from florae are engaged to balance emotions, develop physical and mental well-being [19] by different mechanisms. Therefore, essential oils that are present in the extracts of Desmostachya bipinnata [20] may be responsible for the anti-anxiety activity.
  • 3. Veena et al. Int J Curr Pharm Res, Vol 9, Issue 5, 152-154 154 Further, the anxiolytic effect of flavonoids has been attributed to its effect on the central nervous system and benzodiazepine receptors [21]. It may be possible that the mechanism of anxiolytic action of AQEDB and ALEDB could be due to the binding of any of these phytochemicals to the GABAA-BZD complex. So the anxiolytic activity of AQEDB and ALEDB might involve an action on GABA ergic transmission or effects on serotonergic transmission or due to its mixed aminergic potentiating effect. Spontaneous locomotor activity is considered as an index of alertness and can be helpful to confirm the general depressive activity of any drug. The reduction in motor activity indicates the level of CNS excitability and this may be related to sedation due to CNS depression. However, in the present study, the AQEDB and ALEDB was found to have decreased effect on the locomomotor activity in actophotometer. CONCLUSION The results obtained in this study indicate that the alcoholic and aqueous extracts fractions of the leaves of Desmostacyabipinnata have significant CNS Depressant and Anxiolytic activities in animal model systems. The medicinal values of the plant leaves may be related to their constituent phytochemicals. So, further detailed investigations are needed to isolate and identify the active compounds present in the plant extract and its various fractions. It will help in the development of novel and safe drugs for the treatment of different types of CNS disorders. CONFLICT OF INTERESTS Declare none REFERENCES 1. Reynolds EH. Brain and mind: a challenge for WHO. Lancet 2003;361:1924–25. 2. Patel, Nitirajshree, Praboth V Shah. Evaluation of antidepressant activity of herbomineral formulation. Int J Pharm Pharm Sci 2016;8:1-6. 3. Hina H, Audil R. Isolation of fungi from roots of partheniumhysterophorus and Desmostachyabipinnata and antibacterial activity of their root extracts. 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