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Effect of UV Rays on the Colonial
& Cellular Morphology and
Catalase Activity of Baker’s and
Wild Strains of Yeast
Amna Ja...
Objectives
 Effect of UV on the colonial morphology
of wild as well as baker’s yeast
 Effect of UV on the cellular morph...
Introduction
 UV light -- electromagnetic radiation
 Wavelength shorter -- visible light
 longer -- X-rays
 400 nm and...
 Does not break the DNA chain outright
 Selectively absorbed by the aromatic
rings of the purine and pyrimidine
bases
 ...
Materials and Methods
Collection of the Yeast Sample
1. Soil sample
2. Baker’s Yeast
Spreading on YPD Agar plates
Isola...
Results & Discussion
 Effect of UV on the colonial morphology of
Baker’s Yeast Sample
Property Before UV Exposure
After U...
Colony morphology of Baker’s yeast
before & after UV exposure
Before UV Exposure After UV Exposure
 Effect of UV on the colonial morphology
of wild Yeast Sample
Property Before UV Exposure
After UV Exposure
(1 min)
1. Fo...
Colony morphology of wild yeast before
& after UV exposure
Berore UV Exposure After UV Exposure
Effect of UV on the Cellular
Morphology of Yeast Sample
 The lethal effects of the UV radiation
Mutations of the genes o...
Cellular morphology of Baker’s yeast
before & after UV exposure
Before UV Exposure After UV Exposure
Cellular morphology of wild yeast before
& after UV exposure
Before UV Exposure After UV Exposure
Effect of UV on the Catalase
Activity of Yeast Sample
 UV irradiation on the catalase activity
was divisible into 4 perio...
1. Third, a rather long period during
which irradiation led to no diminution
in the catalase activity of the
maximally act...
Catalase activity of Baker’s yeast before
& after UV exposure
Before UV Exposure After UV Exposure
Catalase activity of wild yeast before &
after UV exposure
Before UV Exposure After UV Exposure
Effect of UV Rays on the Colonial & Cellular Morphology and Catalase Activity of Baker’s and Wild Strains of Yeast
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Effect of UV Rays on the Colonial & Cellular Morphology and Catalase Activity of Baker’s and Wild Strains of Yeast

Effect of UV Rays on the Colonial & Cellular Morphology and Catalase Activity of Baker’s and Wild Strains of Yeast

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Effect of UV Rays on the Colonial & Cellular Morphology and Catalase Activity of Baker’s and Wild Strains of Yeast

  1. 1. Effect of UV Rays on the Colonial & Cellular Morphology and Catalase Activity of Baker’s and Wild Strains of Yeast Amna Jalil Roll No: 17
  2. 2. Objectives  Effect of UV on the colonial morphology of wild as well as baker’s yeast  Effect of UV on the cellular morphology of wild as well as baker’s yeast  Effect of UV on the catalase activity of wild as well as baker’s yeast
  3. 3. Introduction  UV light -- electromagnetic radiation  Wavelength shorter -- visible light  longer -- X-rays  400 nm and 10 nm.
  4. 4.  Does not break the DNA chain outright  Selectively absorbed by the aromatic rings of the purine and pyrimidine bases  Formation of pyrimidine dimers  Two adjacent pyrimidines in the same chain (T-T, C-C, or T-C) become covalently bonded together  These dimers disrupt the local structure of the DNA double helix and prevent normal DNA replication
  5. 5. Materials and Methods Collection of the Yeast Sample 1. Soil sample 2. Baker’s Yeast Spreading on YPD Agar plates Isolation of Pure Strains Exposure of UV Radiations Analyzing the Effect of UV on Colony Morphology and Cellular Morphology Analyzing the effect of UV on Catalase Activity
  6. 6. Results & Discussion  Effect of UV on the colonial morphology of Baker’s Yeast Sample Property Before UV Exposure After UV Exposure (1 min) 1. Form Circular Circular 2. Elevation Convex Raised 3. Margins Entire Entire 4. Color Cream colored Cream colored 5. Surface Smooth & Shiny Rough & Matt
  7. 7. Colony morphology of Baker’s yeast before & after UV exposure Before UV Exposure After UV Exposure
  8. 8.  Effect of UV on the colonial morphology of wild Yeast Sample Property Before UV Exposure After UV Exposure (1 min) 1. Form Circular Circular 2. Elevation Convex Raised 3. Margins Entire Entire 4. Color Cream colored Cream colored 5. Surface Smooth & Shiny Rough & Matt
  9. 9. Colony morphology of wild yeast before & after UV exposure Berore UV Exposure After UV Exposure
  10. 10. Effect of UV on the Cellular Morphology of Yeast Sample  The lethal effects of the UV radiation Mutations of the genes or chromosomes Destruction of the cell-membrane Some structures or cell organelles
  11. 11. Cellular morphology of Baker’s yeast before & after UV exposure Before UV Exposure After UV Exposure
  12. 12. Cellular morphology of wild yeast before & after UV exposure Before UV Exposure After UV Exposure
  13. 13. Effect of UV on the Catalase Activity of Yeast Sample  UV irradiation on the catalase activity was divisible into 4 periods. 1. First, the period during which the cells lost their ability to form colonies, but during which no change in catalase activity was noted. 2. Second, the period during which a considerable rise in catalase activity occurred.
  14. 14. 1. Third, a rather long period during which irradiation led to no diminution in the catalase activity of the maximally active suspension. 2. Fourth, the period of photoinactivation of the intracellular enzyme, which was quite similar to that of the crystalline enzyme in vitro.
  15. 15. Catalase activity of Baker’s yeast before & after UV exposure Before UV Exposure After UV Exposure
  16. 16. Catalase activity of wild yeast before & after UV exposure Before UV Exposure After UV Exposure

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