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GAS CHROMATOGRAPHY-
   MASS SPECTROSCOPY
• MS is a technique in which organic molecules are
  bombarded by electrons or other ionic species
  causing them to ionize and fragment.
• The various ionic species produced by electron
  impact are separated according to mass (strictly
  speaking mass/charge (m/z) ratio) in a magnetic
  field, and a spectrum is obtained that in effect is a
  bar diagram showing the masses of the fragment
  ions and their abundances relative to the most
  abundant ion (base peak) given a value of 100%
GC-MS
GC-MS
• By GC one can get the fair idea of the
  sample (fatty acids), But one is really
  interested in the technique MS is the
  technique which is being used.
• GC-MS combination can be used to detect
  contaminant from spoilage or adulteration
  which may be harmful and controlled by
  government agencies.
TYPICAL MASS SPECTRUM OF
 FAME IS GIVEN AS FOLLOW
GC-MS-MS
• Though GC-MS is better technique to identify any
  compound present in very small amounts,this
  combination is sometime clubed with another
  Mass Detector to get accurate details of compound
  present in nano to pico level.
• This is used when two compounds have same
  retention time.
• Since both compounds are eluting at sametime
  mass spectrum thus obtained will be of both
  compound and thus confusing.
GC-MS-MS
• GC-MS-MS further fragments the ionic
  species fragmented earlier to get a new
  ionic species which is now detected by
  detector(FID).
• This is used in the analysis of foodgrain
  samples to check any pesticide residue with
  its molecular structure.

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Detect Contaminants and Adulterants with GC-MS

  • 1. GAS CHROMATOGRAPHY- MASS SPECTROSCOPY • MS is a technique in which organic molecules are bombarded by electrons or other ionic species causing them to ionize and fragment. • The various ionic species produced by electron impact are separated according to mass (strictly speaking mass/charge (m/z) ratio) in a magnetic field, and a spectrum is obtained that in effect is a bar diagram showing the masses of the fragment ions and their abundances relative to the most abundant ion (base peak) given a value of 100%
  • 3. GC-MS • By GC one can get the fair idea of the sample (fatty acids), But one is really interested in the technique MS is the technique which is being used. • GC-MS combination can be used to detect contaminant from spoilage or adulteration which may be harmful and controlled by government agencies.
  • 4. TYPICAL MASS SPECTRUM OF FAME IS GIVEN AS FOLLOW
  • 5. GC-MS-MS • Though GC-MS is better technique to identify any compound present in very small amounts,this combination is sometime clubed with another Mass Detector to get accurate details of compound present in nano to pico level. • This is used when two compounds have same retention time. • Since both compounds are eluting at sametime mass spectrum thus obtained will be of both compound and thus confusing.
  • 6. GC-MS-MS • GC-MS-MS further fragments the ionic species fragmented earlier to get a new ionic species which is now detected by detector(FID). • This is used in the analysis of foodgrain samples to check any pesticide residue with its molecular structure.