Downstream Processing

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Downstream Processing

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Downstream Processing

  1. 1. www.studentyogi.com www.studentyogi.com Code No: R05412304 Set No. 1 IV B.Tech I Semester Regular Examinations, November 2008 DOWNSTREAM PROCESSING (Bio-Technology) Time: 3 hours Max Marks: 80 Answer any FIVE Questions All Questions carry equal marks 1. Describe about economic assessment of various proteins via r-DNA. [16] 2. (a) What are the parameters used in characterization in fermentation broth? (b) List down all the important impurities and containments present in DSP and their removal techniques. [16] 3. Write short notes on the following. om (a) Enzymatic cell lysis (b) Chemical cell lysis (c) Concentration polarization and cross ow ltration [16] i.c 4. Write short notes on : (a) Liquid membranes (b) Ultra ltration. [16] g 5. (a) Write about the criteria used for selection of extraction equipment in antibiotic industry. omyo (b) A compound X is to be extracted from a clari ed fermentation beer by using pure amyl acetate as solvent at pH 4.0. The distribution coe cient K of the system was found to be 30. The initial concentration of compound x in the feed is 400ml/L. The ow rates of the feed and solvent streams are 500L/H t and 30L/hr respectively. How many ideal stages are required to recover 90% i.cen of X in the feed. [8+8] 6. Write the mode of separation of compounds by capillary electrophoresis. Discuss the mode of operation by a schematic diagram. [16] otgud 7. Write short notes on: (a) Peak asymmetry (b) Selectivity factor nty.s (c) Stationary phase (d) Gradient elution. [4×4=16] de w 8. Discuss the pro cess of crystallization. What are the di erent parameters considered before crystalliaing a compound? [16] w 1 of 2 stuw www.studentyogi.com www.studentyogi.com w. ww
  2. 2. www.studentyogi.com www.studentyogi.com Code No: R05412304 Set No. 1 om i.c og nty de u w .st ww 2 of 2 www.studentyogi.com www.studentyogi.com
  3. 3. www.studentyogi.com www.studentyogi.com Code No: R05412304 Set No. 2 IV B.Tech I Semester Regular Examinations, November 2008 DOWNSTREAM PROCESSING (Bio-Technology) Time: 3 hours Max Marks: 80 Answer any FIVE Questions All Questions carry equal marks 1. What is the role of pro cess engineer in Bioseparation process? Explain in detail with help of suitable examples. [16] 2. What are the ma jor steps involved in the product isolation and puri cation of citric acid manufacturer? [16] 3. (a) Discuss the theoretical principles of constant pressure ltration. om (b) How is compressibility of a cake determined? [16] 4. Explain in detail how do you classify the membrane separation methods according to particle size. [16] 5. What is integrated bioprocessing. Draw a neat schematic diagram for a known i.c compound of your interest . What are the likely problems encountered in the pro cess and the necessary steps to overcome them to upkeep the process e ciency. [16] of an unknown protein on the gel. og 6. Write the procedure of SDS-PAGE. Discuss how to calculate the molecular weight [16] 7. (a) Write the principle of Gel chromatographu. nty (b) What is the retention volume (VR) if external solution (V0) is 16ml and inter- nal solution is 5 ml and fraction of (Vi ) acceptable to solute is 12ml in a gel chromatography column? [16] 8. (a) Write about the process of crystal formation and the geometrical changes de associated with it (b) Write the signi cance of crystallization in pro duct recovery. [8+8] u w .st ww 1 of 1 www.studentyogi.com www.studentyogi.com
  4. 4. www.studentyogi.com www.studentyogi.com Code No: R05412304 Set No. 3 IV B.Tech I Semester Regular Examinations, November 2008 DOWNSTREAM PROCESSING (Bio-Technology) Time: 3 hours Max Marks: 80 Answer any FIVE Questions All Questions carry equal marks 1. Discuss about the classical and modern biotechnology consideration of downstream pro cessing in Biotech industry. [16] 2. Discuss the steps involved the product isolation and puri cation of an antibiotic pro duction. [16] 3. Write notes on the operation of tubular bowl centrifuge and disc stack bowl cen- om trifuge. [16] 4. (a) Discuss about the classi cation of membrane separation process. (b) What are the transport models for membrane process? Explain about them. i.c [16] 5. Explain “in situ product removal as a tool for bioprocessing”. [16] 6. (a) Describe about di erent types of support media employed in electrophoresis. og (b) How to prepare the sample of a nucleic acid to load on an Agarose gel. [16] 7. (a) Write about di erent types of Stationary phases available for Gas chromatog- raphy. nty (b) What is the percentage composition of the mixture of Ethane, Propane, butane if in Gas chromatography separation the peak aeas were 53.2, 14.5 and 31 cm. [16] 8. Write the principle and process of Crystallization. Discuss how it is di erent from de precipitation. [16] u w .st ww 1 of 1 www.studentyogi.com www.studentyogi.com
  5. 5. www.studentyogi.com www.studentyogi.com Code No: R05412304 Set No. 4 IV B.Tech I Semester Regular Examinations, November 2008 DOWNSTREAM PROCESSING (Bio-Technology) Time: 3 hours Max Marks: 80 Answer any FIVE Questions All Questions carry equal marks 1. Give generalized pro cess recovery scheme for Enzyme from plant source with help of solid state fermentation. [16] 2. Write in detail about the selecting of various unit operations in relation to their separation factor and the molecular size of the material to be recovered in DSP. [16] om 3. (a) Explain the principle of centrifugal separation. (b) What is a gyro tester? What are its uses? [16] 4. (a) What are the advantages of membrane separation pro cess? (b) What is Ultra ltration? How is it useful in bio separation? [16] i.c 5. What is in situ product removal in bioprocessing. What are the disadvantages of using ISPR and the necessary precautions in ISPR operation. [16] 6. Short notes on (a) Capillary array electrophoresis (b) Isoelectric focusing og nty (c) Capillary zone electrophoresis (d) Electro osmotic ow. [4×4] 7. Write short notes on: (a) Temperature programming in GC de (b) Band separation (c) Electron capture detector (d) Open tubular columns. [4×4] u 8. What is crystallization and how it is common and di erent from lyophilisation. .st [16] w ww 1 of 1 www.studentyogi.com www.studentyogi.com

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