Signature Program: Reproductive Sciences
Reproductive Sciences Laboratory (RSL) <ul><li>Faculty </li></ul><ul><li>Mark Westhusin </li></ul><ul><li>Duane Kraemer </...
Affiliations <ul><li>Interdisciplinary Faculty of Reproductive Biology (IFRB) </li></ul><ul><li>42 Members, 4 Colleges </l...
Nucleus Transfer <ul><li>Collecting and storing nucleus donor cells </li></ul><ul><ul><li>Surgical procedure </li></ul></u...
The Technology <ul><li>Preparing nuclei for transfer </li></ul><ul><ul><li>Regulating cell cycle </li></ul></ul><ul><ul><u...
The Technology <ul><ul><li>Micromanipulation </li></ul></ul>
Nucleus Transfer <ul><ul><li>Enucleating ova  (removing the  nucleus) </li></ul></ul>
The Technology
The Technology <ul><ul><li>Transfer of the cell </li></ul></ul>
The Technology
The Technology <ul><ul><li>Cell fusion </li></ul></ul>
Cell Fusion
Cell Fusion
The Technology <ul><ul><li>Alternative Method </li></ul></ul><ul><ul><ul><li>Intracytoplasmic  nucleus injection </li></ul...
The Technology <ul><li>DNA Reprogramming </li></ul><ul><ul><li>Methylation </li></ul></ul><ul><ul><li>Demethylation </li><...
The Technology <ul><li>Activation </li></ul><ul><ul><li>Electrical </li></ul></ul><ul><ul><li>Chemical </li></ul></ul><ul>...
Examples of Clones <ul><li>Second Chance </li></ul><ul><li>21 year-old Brahman Bull </li></ul>Photos courtesy of: Hill, et...
Second Chance (3 years old)
Examples of Clones <ul><li>86 Squared </li></ul><ul><ul><li>Brucellosis  resistant bull </li></ul></ul><ul><ul><li>Tissue ...
86 Squared (2 years old)
Examples of Clones
White-tailed Deer (Dewey)
 
Dewey 16 months old
Examples of Clones <ul><li>CC – The first cloned cat </li></ul>Progenitor
Current Research Projects: <ul><li>In Utero stem cell trasplantation to correct hemophilia A. NIH subcontract through Univ...
Objective: <ul><li>To re-establish and extinct strain of hemophilic sheep from 6 straws of frozen semen </li></ul><ul><li>...
Full Spectrum of Reproducive Technologies <ul><li>Multiple Ovulation Embryo Transfer (MOET) </li></ul><ul><li>Artificial I...
 
 
Rehearsal Experiment <ul><li>Procedures </li></ul><ul><li>MOET </li></ul><ul><li>MOET+NTC </li></ul><ul><li>IVF </li></ul>...
First Experiment <ul><li>Procedures </li></ul><ul><li>MOET </li></ul><ul><li>IVF </li></ul><ul><li>ICSI </li></ul><ul><li>...
Research Projects <ul><li>Somatic cell nucleus transfer in the canine. Texas Heart Institute. $72,721. P.I. D.C. Kraemer, ...
Canine Cloning <ul><li>9 bitches </li></ul><ul><ul><li>Huntsville (Labradors and hounds) </li></ul></ul><ul><ul><li>Local ...
Procedure <ul><li>Oocyte collection and  in vitro  maturation (IVM) </li></ul>
Results to Date <ul><li>IVM- collected average 64 MI and MII </li></ul><ul><li>Transfer average 36 couplets </li></ul><ul>...
 
Upcoming SlideShare
Loading in …5
×

08 Reproductive Sciences

405 views

Published on

Published in: Technology, Health & Medicine
  • Be the first to comment

  • Be the first to like this

08 Reproductive Sciences

  1. 1. Signature Program: Reproductive Sciences
  2. 2. Reproductive Sciences Laboratory (RSL) <ul><li>Faculty </li></ul><ul><li>Mark Westhusin </li></ul><ul><li>Duane Kraemer </li></ul><ul><li>Charles Long </li></ul><ul><li>Visiting Scientist </li></ul><ul><li>Mundhir Ridha </li></ul><ul><li>Staff </li></ul><ul><li>Taeyoung Shin </li></ul><ul><li>Christy Bormann </li></ul><ul><li>Graduate Students </li></ul><ul><li>Carrie Hanna </li></ul><ul><li>Jie Liu </li></ul><ul><li>Arantza Lassala </li></ul><ul><li>Jessica Epple-Farmer </li></ul><ul><li>Abigail Casillo </li></ul><ul><li>Lara Charles </li></ul><ul><li>Beth Kidd-White </li></ul><ul><li>Suzanne Mengas </li></ul><ul><li>James Williams </li></ul>
  3. 3. Affiliations <ul><li>Interdisciplinary Faculty of Reproductive Biology (IFRB) </li></ul><ul><li>42 Members, 4 Colleges </li></ul><ul><li>11 – CVM, 6 – Joint Appointments in CVM </li></ul><ul><li>Center for Genomics and Biotechnology </li></ul>
  4. 4. Nucleus Transfer <ul><li>Collecting and storing nucleus donor cells </li></ul><ul><ul><li>Surgical procedure </li></ul></ul><ul><ul><ul><li>Live animal </li></ul></ul></ul><ul><ul><ul><li>Post mortem </li></ul></ul></ul><ul><ul><li>Culturing cells </li></ul></ul><ul><ul><li>Cryopreservation (freezing) of cells </li></ul></ul>
  5. 5. The Technology <ul><li>Preparing nuclei for transfer </li></ul><ul><ul><li>Regulating cell cycle </li></ul></ul><ul><ul><ul><li>Serum starvation </li></ul></ul></ul><ul><li>Obtaining recipient ova </li></ul><ul><ul><li>In vivo matured </li></ul></ul><ul><ul><li>In vitro matured </li></ul></ul>
  6. 6. The Technology <ul><ul><li>Micromanipulation </li></ul></ul>
  7. 7. Nucleus Transfer <ul><ul><li>Enucleating ova (removing the nucleus) </li></ul></ul>
  8. 8. The Technology
  9. 9. The Technology <ul><ul><li>Transfer of the cell </li></ul></ul>
  10. 10. The Technology
  11. 11. The Technology <ul><ul><li>Cell fusion </li></ul></ul>
  12. 12. Cell Fusion
  13. 13. Cell Fusion
  14. 14. The Technology <ul><ul><li>Alternative Method </li></ul></ul><ul><ul><ul><li>Intracytoplasmic nucleus injection </li></ul></ul></ul>
  15. 15. The Technology <ul><li>DNA Reprogramming </li></ul><ul><ul><li>Methylation </li></ul></ul><ul><ul><li>Demethylation </li></ul></ul>
  16. 16. The Technology <ul><li>Activation </li></ul><ul><ul><li>Electrical </li></ul></ul><ul><ul><li>Chemical </li></ul></ul><ul><ul><ul><li>Insitol 1,4, 5-triphosphate </li></ul></ul></ul><ul><ul><ul><li>Ionomycin + b-dimethylaminopurine </li></ul></ul></ul>
  17. 17. Examples of Clones <ul><li>Second Chance </li></ul><ul><li>21 year-old Brahman Bull </li></ul>Photos courtesy of: Hill, et al., Biology of Reproduction 62:1135 (2000)
  18. 18. Second Chance (3 years old)
  19. 19. Examples of Clones <ul><li>86 Squared </li></ul><ul><ul><li>Brucellosis resistant bull </li></ul></ul><ul><ul><li>Tissue frozen for 15 years </li></ul></ul>
  20. 20. 86 Squared (2 years old)
  21. 21. Examples of Clones
  22. 22. White-tailed Deer (Dewey)
  23. 24. Dewey 16 months old
  24. 25. Examples of Clones <ul><li>CC – The first cloned cat </li></ul>Progenitor
  25. 26. Current Research Projects: <ul><li>In Utero stem cell trasplantation to correct hemophilia A. NIH subcontract through University of Nevada, Reno, $680,500. P.I. of Subcontract: D.C. Kraemer, 2003-2007 </li></ul>
  26. 27. Objective: <ul><li>To re-establish and extinct strain of hemophilic sheep from 6 straws of frozen semen </li></ul><ul><li>Work done at U.N.-Reno (mobile lab) </li></ul>
  27. 28. Full Spectrum of Reproducive Technologies <ul><li>Multiple Ovulation Embryo Transfer (MOET) </li></ul><ul><li>Artificial Insemination (AI) </li></ul><ul><li>In-Vitro Oocyte maturation (IVM) </li></ul><ul><li>In-Vitro Fertilization (IVF) </li></ul><ul><li>Intra Cytoplasmic Sperm Injection (I CSI) </li></ul><ul><li>Nucleus Transfer Cloning (NTC) </li></ul>
  28. 31. Rehearsal Experiment <ul><li>Procedures </li></ul><ul><li>MOET </li></ul><ul><li>MOET+NTC </li></ul><ul><li>IVF </li></ul><ul><li>ICSI </li></ul><ul><li>NTC </li></ul>Transfers 4 4 3 2 2 15 Pregnancies 3 2 0 0 0 5
  29. 32. First Experiment <ul><li>Procedures </li></ul><ul><li>MOET </li></ul><ul><li>IVF </li></ul><ul><li>ICSI </li></ul><ul><li>NTC </li></ul>Transfers 0 0 17 2 19 Pregnancies 0 0 11 0 11
  30. 33. Research Projects <ul><li>Somatic cell nucleus transfer in the canine. Texas Heart Institute. $72,721. P.I. D.C. Kraemer, 2004-2005 </li></ul>
  31. 34. Canine Cloning <ul><li>9 bitches </li></ul><ul><ul><li>Huntsville (Labradors and hounds) </li></ul></ul><ul><ul><li>Local (Basset x Dalmatian) </li></ul></ul>
  32. 35. Procedure <ul><li>Oocyte collection and in vitro maturation (IVM) </li></ul>
  33. 36. Results to Date <ul><li>IVM- collected average 64 MI and MII </li></ul><ul><li>Transfer average 36 couplets </li></ul><ul><li>No full term pregnancies… so far </li></ul>

×