Curriculum vitae federico sabbadin

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Curriculum vitae federico sabbadin

  1. 1. CURRICULUM VITAE PERSONAL INFORMATION Name Federico Sabbadin Address 2 Wellington Street, YO10 5BD, York, UK Telephone Personal: (44) 01904629363 Mobile: (44) 07799116030 E-mail fs525@york.ac.uk Nationality Italian Date of birth 7 March 1983 Gender Male WORK EXPERIENCE Dates October 2007 – present Name and address of employer Centre for Novel Agricultural Products (CNAP), Department of Biology, University of York, United Kingdom York, YO10 5YW (44) 01904328783 Position held PhD Student Main activities/skills covered - designing a platform for high-throughput generation of libraries of chimeric P450s for biocatalysis and characterization of orphan P450s - developing new high throughput methods to screen the activity of NAD(P)H-dependent enzymes over panels of potential substrates - X-ray crystallography of XplA, an explosive degrading P450 - site-directed mutagenesis of XplA, characterization and kinetic analysis of the mutants - directed evolution of XplA P450 towards the degradation of the explosive pollutant HMX - techniques: PCR, gene cloning, DNA primer design and in silico DNA analysis, whole cell and in vitro activity assays, protein expression in Escherichia coli host, purification via metal affinity chromatography and gel filtration chromatography, SDS-PAGE, Western Blot, FPLC, HPLC, GC-MS, protein crystallization techniques (vapour diffusion), enzyme kinetics
  2. 2. Dates October 2006 – July 2007 Name and address of employer Agripolis Campus, Viale dell`Universita` 16 -35020- Legnaro, Padova, Italy (39) 049 827 25 35 Position held Master`s Degree Student Main activities/skills covered - site directed mutagenesis of the polygalacturonase enzyme produced by the pathogenic fungus Fusarium verticilllioides - activity analysis of the poly-galacturonase mutants - techniques: DNA primer design, PCR, site directed mutagenesis, FPLC, isoelectric facousing, Escherichia coli and Pichia pastoris transformation (heat shock and PEG techniques), expression of heterologous proteins in the methylotrophic yeast Pichia pastoris, bacterial and yeast culture Dates May – July 2005 Name and address of employer Agripolis Campus, Viale dell`Universita` 16 -35020- Legnaro, Padova, Italy (39) 049 827 25 35 Position held Trainee Main activities/skills covered - immunolocalization of AtPIN1 auxin carrier in maize plant - in-situ hybridization of AtPIN1 auxin carrier in maize plant - radioactive Northern Blotting of AtPIN1 auxin carrier mRNA REFEREED PAPERS 1. Sabbadin, F., Hyde, R., Robin, A., Hilgarth, E., Delenne, M., Flitsch, S., Turner, N., Grogan, G., and Bruce, N. C., 2010. LICRED: a versatile drop-in vector for rapid generation of redox-self-sufficient cytochrome P450s. ChemBioChem (accepted, in press). 2. Sabbadin, F., Jackson, R., Haider, K., Tampi, G., Turkenburg, J. P., Hart, S., Bruce, N. C., and Grogan, G., 2009. The 1.5-Å structure of XplA-heme, an unusual cytochrome P450 heme domain that catalyzes reductive biotransformation of the Royal Demolition Explosive. J. Biol. Chem., 284: 28467-28475. 3. Robin, A., Roberts., G. A., Kisch, J., Sabbadin, F., Grogan, G., Bruce, N. C., Turner, N. J., and Flitsch, S. L., 2009. Engineering and improvement of the efficiency of a chimeric [P450cam-RhFRed reductase domain] enzyme. Chem. Comm., 18: 2478-2480.
  3. 3. AWARDS Best Poster Award. 16th International Conference on Cytochrome P450, 2009. Nago, Okinawa, Japan. EDUCATION AND TRAINING Dates October 2005 – July 2007 Name and type of organisation University of Padova, Padova, Italy providing education and training Principal subjects/occupational - plant biology, physiology, ecology, pathology skills covered - molecular biology, genetics - standard laboratory chemical techniques - plasmidic DNA extraction, purification and quantification - DNA primer design, PCR amplification, sequencing, aligning Title of qualification awarded Master’s Degree in Nutrition Biotechnologies Level in national classification ISCED 5 Vote obtained 110/110 with honors Dates October 2002 – October 2005 Name and type of organisation University of Padova, Padova, Italy providing education and training Principal subjects/occupational - plant biology, physiology, ecology, pathology skills covered - molecular biology, genetics Title of qualification awarded Bachelor of Science in Agriculture Biotechnologies Level in national classification ISCED 5 Vote obtained 110/110 with honors Dates September 1997 – July 2002 Name and type of organisation Scientific Liceum “E. Curiel”, via Durer 14 -35132- Padova, Italy providing education and training Principal subjects/occupational - biology skills covered - mathematics - physics - chemistry Title of qualification awarded Diploma Level of national classification ISCED 3 Vote obtained 100/100 with honors
  4. 4. PERSONAL SKILLS AND COMPETENCES MOTHER LANGUAGE Italian OTHER LANGUAGES English Reading skills excellent Writing skills excellent Verbal skills excellent French Reading skills good Writing skills good Verbal skills good SOCIAL SKILLS Team work: I have worked as a pizza chef for 5 months, collaborating with the restaurant personnel. I have also practiced team sports (basket, volleyball) TECHNICAL SKILLS AND Competent with most Microsoft Office programmes COMPETENCES OTHER SKILLS AND - certified PADI Open Water Diver COMPETENCES - athletics and fitness - amateur home microscopy DRIVING LICENCE(S) Cathegory B vehicle

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