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The+ Zebrafish+ Project


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The+ Zebrafish+ Project

  1. 1. The Zebrafish Project Katie Hengen Lesley Sullivan The Zebrafish Project Lesley Sullivan Katie Hengen
  2. 2. Zebrafish <ul><li>Danio rerio, freshwater fish </li></ul><ul><li>Originally from Ganges River in East India and Burma </li></ul><ul><li>Found in slow streams and rice paddies </li></ul><ul><li>Excellent model for studying vertebrate development and genetics </li></ul>
  3. 3. Used in Research <ul><li>Dr. George Streisinger (1970’s) determined usefulness </li></ul><ul><li>Similar embryonic development to humans </li></ul><ul><li>Human genome known, easy to work with similar genes </li></ul><ul><li>Some mutants known to develop and duplicate certain diseases common to humans </li></ul>
  4. 4. Ideal Models <ul><li>Vertebrates </li></ul><ul><ul><li>Similar biological traits to humans </li></ul></ul><ul><ul><ul><li>Genes </li></ul></ul></ul><ul><ul><ul><li>Developmental processes </li></ul></ul></ul><ul><ul><ul><li>Anatomy </li></ul></ul></ul><ul><ul><ul><li>Physiology </li></ul></ul></ul><ul><ul><ul><li>Behaviors </li></ul></ul></ul><ul><ul><li>Better genetic models than commonly used invertebrates (fruit flies, worms) </li></ul></ul>
  5. 5. Ideal Models <ul><li>Certain features make easy to maintain, manipulate, and observe </li></ul><ul><ul><li>Do well in many environments </li></ul></ul><ul><ul><li>Small size </li></ul></ul><ul><ul><li>Ability to be kept in large numbers </li></ul></ul><ul><ul><li>Easy breeding </li></ul></ul><ul><ul><ul><li>Eggs externally fertilized </li></ul></ul></ul><ul><ul><ul><li>Produced regularly in large numbers </li></ul></ul></ul><ul><ul><ul><li>Non-adhesive </li></ul></ul></ul><ul><ul><li>Easy to trace development of individual cells </li></ul></ul>
  6. 6. Ideal Models <ul><li>Large quantities of eggs </li></ul><ul><li>Develop outside mother’s body </li></ul><ul><ul><li>Easily accessible, contrasting mice </li></ul></ul><ul><li>Transparent embryos </li></ul><ul><ul><li>Observe very first cell division </li></ul></ul><ul><ul><li>Watch development of internal organs </li></ul></ul><ul><ul><li>Monitor behavior of single cells </li></ul></ul><ul><li>Embryos develop quickly </li></ul><ul><ul><li>From single cell to fish-like in 24 hours </li></ul></ul>Zebrafish eggs 2 hours after fertilization.
  7. 7. Ideal Models <ul><li>Can physically manipulate embryos </li></ul><ul><ul><li>Transplant cells/groups of cells into host embryo </li></ul></ul><ul><ul><li>Fertilization manipulated so embryo contains only mother’s genes </li></ul></ul><ul><li>Large community of researchers work with zebrafish </li></ul>
  8. 8. Zebrafish in High Schools <ul><li>Inexpensive </li></ul><ul><li>Tolerant of reasonable amount of stress </li></ul><ul><li>Produce large amounts of eggs for observation </li></ul><ul><li>Clear embryo visual with standard high school microscopes </li></ul><ul><li>Fish embryo development similar to other species </li></ul><ul><li>Zebrafish are entertaining; students enjoy working with them </li></ul>
  9. 9. Research Using Zebrafish <ul><li>Embryology </li></ul><ul><li>Collecting and analyzing mutations, comparing to other vertebrates </li></ul><ul><li>Genetic techniques and staining reagents developed </li></ul><ul><li>Zebrafish genome </li></ul><ul><li>Transgenic fish </li></ul>
  10. 10. Reaching Out <ul><li>Plans unfolding </li></ul><ul><li>Biology students at St. Joe are VERY LUCKY! </li></ul><ul><li>Clay High School </li></ul><ul><li>QuarkNet </li></ul><ul><li>NDerc </li></ul>
  11. 13. Thanks for 'watching'!