Geno Toxicity Presented by Subin Joy Koikkara Senior Reserch Guide, A.I.M.S Reserch Centre
Dr. V.V. Pillay
Dept of Analytical Toxicology
Co-Guide Internal Guide
Mr.Subin joy Dr. E. G. WESELY
HEAD & READER
Senior Scientific Guide,
Dept of Analytical Toxicology Biotechnology,
AIMS, Kerala. MCAS, Rasipuram
Modern chemicals, leads to increased pollution of ecosystems.
Insecticides – Organochlorine, Organophosphate, Carbamates.
Carbamates – derivatives of carbamic acid
• Mode of action – inhibit cholinesterase enzyme AChE
overstimulation of neuromuscular
leads to spasms
• Symptoms – Headache, Dizziness, weakness ,
excessive salivation , vomiting.
• Genetic toxicology – effects of chemicals on the hereditary material .
Comet assay – versatile, novel ,rapid but highly sensitive and well validated tool for detecting DNA
damage in single cells.
Aim& Obj ective
Detection of DNA damage by Alkaline comet assay in peripheral
To induce the blood with different carbamate pesticides.
To check whether carbamate pesticide will cause genotoxicity in human
Detection of DNA damage by Fluorescent microscope.
Human blood sample Carbaryl
•Cold slide tray
The word comet means (originated from
Greek – kometes) -‘long haired star’.
This single cell gel electrophoresis technique •Department of Bioengineering
University of Washington
has been modified and extensively validated Health Sciences Complex,
G-103 Box 357962
over the years, and is now commonly referred Seattle, WA 98195 , U.S.A.
to as the ‘comet’ assay
Preparation of carbamate stock solutions
4 mg of three carbamates are dissolved in 1ml of 1%DMSO
Methomyl: 1600 ppm/ml
Carbaryl: 2000 ppm/ml
Carbendazim:2000 ppm /ml
Carbamates Stocksolution 1%DMSO Totoal
(ml) (ml) concentration
Methomyl 0.5 0.5 800
Carbaryl 0.5 0.5 1000
Carbendazim 0.5 0.5 1000
suggest that pesticide in long term exposure could acts as clastogens
on the DNA of somatic cells.
(Methomyl & carbaryl) and one fungicides (Carbendazim) showed
some genotoxic effects at very low concentration in leukocytes
So these carbamate pesticides are consider as non-target