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Iso Allo Idio 2001


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Iso Allo Idio 2001

  1. 1. Immunoglobulin: Isotypes, Allotypes and Idiotypes
  2. 2. Immunoglobulin Isotypes <ul><li>Definition - Antigenic determinants that characterize the classes and subclasses of heavy chains and types and subtypes of light chains </li></ul>
  3. 3. Human IgM antiserum Reacts with IgM, IgG, IgA, IgD, IgE React with IgG, IgA, IgD and IgE Absorbed antiserum Reacts only with IgM Recognizes determinants specific for  heavy chains (isotypes) Immunoglobulin Isotypes ?? ?? ??
  4. 4. Immunoglobulin Isotypes <ul><li>Occurrence </li></ul><ul><li>Importance </li></ul><ul><ul><li>Ig levels </li></ul></ul><ul><ul><li>B cell tumors </li></ul></ul><ul><ul><li>Immunodeficiencies </li></ul></ul><ul><li>Definition </li></ul><ul><li>Location </li></ul>IgM (kappa) IgG1 (kappa)
  5. 5. Immunoglobulin Allotypes <ul><li>Definition - Antigenic determinants specified </li></ul><ul><li>by allelic forms of the Ig genes </li></ul><ul><li>Location </li></ul><ul><li>Occurrence </li></ul><ul><li>Source of anti-allotypic Abs </li></ul>IgG1 (kappa) Person 2 IgG1 (kappa) Person 1
  6. 6. Human Immunoglobulin Allotypes <ul><li>Nomenclature </li></ul><ul><ul><li>G1m(3) </li></ul></ul><ul><ul><li>Km(1) </li></ul></ul>
  7. 7. Human Immunoglobulin Allotypes <ul><li>Genetics (alleles) </li></ul><ul><ul><li>Co-dominant </li></ul></ul><ul><ul><li>autosomal genes </li></ul></ul>Km(1)/Km(3) X Km(1)/Km(1) Km(1)/Km(1) and Km(1)/Km(3) <ul><li>Allelic exclusion </li></ul>
  8. 8. <ul><li>Genetics (pseudoalleles) </li></ul><ul><ul><li>Co-dominant </li></ul></ul>Human Immunoglobulin Allotypes G1m(17) G1m(1) 214 355-358
  9. 9. Immunoglobulin Allotypes <ul><li>Definition </li></ul><ul><li>Location </li></ul><ul><li>Occurrence </li></ul><ul><li>Importance </li></ul><ul><ul><li>Monitoring bone marrow grafts </li></ul></ul><ul><ul><li>Forensic medicine </li></ul></ul><ul><ul><li>Paternity testing </li></ul></ul>
  10. 10. Immunoglobulin Idiotypes <ul><li>Definition - Unique antigenic determinants present on individual antibody molecules or on molecules of identical specificity </li></ul>
  11. 11. DNP-BSA Strain A Mouse Anti-DNP Ab Purified anti-DNP Ab Strain A Mouse Ab against combining site of anti-DNP Ab ?? Immunoglobulin Idiotypes Antigenic determinants created by the HVR = Idiotypes
  12. 12. Immunoglobulin Idiotypes <ul><li>Definition </li></ul><ul><li>Location </li></ul>IgG1 (kappa) Person 1 anti-B IgG1 (kappa) Person 1 anti-A
  13. 13. Immunoglobulin Idiotypes <ul><li>Definition </li></ul><ul><li>Location </li></ul><ul><li>Importance </li></ul><ul><ul><li>V region marker </li></ul></ul><ul><ul><li>Regulation of immune responses </li></ul></ul>
  14. 14. Ag Id anti-Id + - anti-anti-Id + - Regulation by Id/anti-Id
  15. 15. Immunoglobulin Idiotypes <ul><li>Definition </li></ul><ul><li>Location </li></ul><ul><li>Importance </li></ul><ul><ul><li>V region marker </li></ul></ul><ul><ul><li>Regulation of immune responses </li></ul></ul><ul><ul><li>Vaccines </li></ul></ul>
  16. 16. Anti-Idiotype Vaccines Ag Id anti-Id + - Ag Id anti-Id +
  17. 17. Immunoglobulin Idiotypes <ul><li>Definition </li></ul><ul><li>Location </li></ul><ul><li>Importance </li></ul><ul><ul><li>V region marker </li></ul></ul><ul><ul><li>Regulation of immune responses </li></ul></ul><ul><ul><li>Vaccines </li></ul></ul><ul><ul><li>Treatment of B cell tumors (T cell) </li></ul></ul>