MAG

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MAG

  1. 1. Evaluation of antimicrobial properties of MAG<br />Gabriel Costa<br />09/11/2009<br />
  2. 2. Outline<br />Introduction<br />Aim<br />Methods<br />Results<br />Problems<br />Conclusion<br />Acknowledgments<br />
  3. 3. Introduction<br />
  4. 4. Introduction<br />Monoacylglycerols<br />CARACHTERISTICS<br /><ul><li> Partial esters of glycerol
  5. 5. Non-ionic surface-active compounds
  6. 6. Biodegradability</li></ul>APPLICATION<br /><ul><li> Food, cosmetic, textile, plastic industry, etc.</li></ul>PREPARATION<br /><ul><li> Interesterification
  7. 7. Hydrolysis of triacylglycerols
  8. 8. Fatty acid addition to glycidol</li></li></ul><li>Introduction<br />Nisin<br />CARACHTERISTICS<br /><ul><li> Polycyclic peptide antibacterial with 34 aminoacids
  9. 9.  Rare example of a broad-spectrum bacteriocin (effective against gram positives and gram negatives)</li></ul>APPLICATION<br /><ul><li> Foodindustry as foodpreservative</li></ul>PREPARATION<br /><ul><li> Fermentation usingLactococcuslactis</li></li></ul><li>Aim<br />
  10. 10. Evaluate antimicrobial effect of <br />monoacylglycerol <br />Lauricacid<br />Palmitic acid<br />Adamantanecarboxylic acid<br />Aim<br />Nisin<br />
  11. 11. Evaluate of antimicrobial effect of <br />monoacylglycerol <br />Lauricacid<br />Palmitic acid<br />Adamantanecarboxylic acid<br />Aim<br />MAG A<br />Nisin<br />
  12. 12. Methods<br />
  13. 13. 1-Adamantanecarboxylic acid <br />+ <br />toluene (8ml per 1 gram)<br />Lauric acid <br />or <br />palmiticacid<br />glass reactor with a temperature-stabilized jacket<br />96 ⁰C 30 minutes<br />Glycidol <br />acid:glycidol (1:1,8)<br />Purification and Filtration<br />Methods<br />Preparation of MAG<br />Solvent system<br />Non-solvent system<br /> Chromium (III) acetate hydroxide0,5% w/w<br />96 ⁰C 30 minutes<br />96 ⁰C 120 minutes<br />
  14. 14. Methods<br />Antimicrobialassay<br />Bacillus subtilis<br />Staphylococcus aureus<br />Bacillus cereus<br />Micrococcus luteus<br />EnterococcusFaecalis<br />Gram +<br />Micrococcus luteus CCM 732<br />Escherichia coli<br />Citrobacterfreundii<br />Serratiamarcescens<br />Salmonella enterica<br />Pseudomonas aeruginosa<br />Gram -<br />Salmonella enterica ssp. Enterica ser. Enteritidis CCM 4420<br />
  15. 15. Methods<br />Antimicrobialassay<br />Bacillus subtilis<br />Staphylococcus aureus<br />Bacillus cereus<br />Micrococcus luteus<br />EnterococcusFaecalis<br />Gram +<br />Micrococcus luteus CCM 732<br />Escherichia coli<br />Citrobacterfreundii<br />Serratiamarcescens<br />Salmonella enterica<br />Pseudomonas aeruginosa<br />Gram -<br />Salmonella enterica ssp. Enterica ser. Enteritidis CCM 4420<br />
  16. 16. Methods<br />Antimicrobialassay<br /> Dilution of MAG in MPB<br />(25 - 1500 µl/ml)<br /> 200µl MPB with MAG + 5 µl of bacterial suspension<br />Incubation in plate reader<br />Absorbance readings (600nm)<br />Every 30 minutes24 hours<br />Growth curves<br />
  17. 17. Results<br />
  18. 18. Results<br />Gram +<br />
  19. 19. Results<br />Gram +<br />
  20. 20. Results<br />Gram +<br />
  21. 21. Results<br />Bacillussubtilis<br />
  22. 22. Results<br />Bacillussubtilis<br />41%<br />
  23. 23. Results<br />Bacilluscereus<br />
  24. 24. Results<br />Petridishes<br />Used to confirmtheresults<br />MAG A of Iva-1000µg/ml<br />MAG A of Gabriel - 1000 µg/ml<br />Nisin - 500µg/ml<br />MAG A of Iva + Nisin–100MAG + 300N µg/ml<br />MAG A of Gabriel + Nisin-100MAG + 300N µg/ml<br />MAG Lauric - 1000µg/ml<br />MAG A of Gabriel + Nisin - 200MAG + 200N µg/ml<br />
  25. 25. Problems<br />
  26. 26. Problems<br />Wealwayshave...<br />MAG A<br /><ul><li>Didnot work thefirst time
  27. 27. 10 days to realize and prepare anotherone</li></ul>PLATE READER<br /><ul><li>Light bulbburned out
  28. 28. Couldnottest MAG of palmiticacid
  29. 29. Couldnottest mix (Nisin + MAG) in gramnegativebacterias</li></li></ul><li>Conclusions<br />
  30. 30. Conclusions<br />COMBINATION<br /><ul><li>Probablesynergisticeffectbetweennisinand MAG
  31. 31. Othercombinations are possiblyeffective
  32. 32. Part of nisincouldbereplacedby MAG to reducecosts</li></li></ul><li>Acknowledgments<br />
  33. 33. Acknowledgments<br />Eva Svobodová<br />LenkaPlechačová<br />Olga Hauková<br />HankaMiklíková<br />
  34. 34. Special acknowledgments<br />

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