Successfully reported this slideshow.

Gail donegan's html cv

915 views

Published on

For full functionality and links, visit HTML version at
http://www.gaildonegan.site90.net/

Published in: Business, Technology
  • Be the first to comment

  • Be the first to like this

Gail donegan's html cv

  1. 1. Professional Profile http://www.gaildonegan.site90.net/ Gail Donegan: Curriculum Vitae Email: gcdonegan@yahoo.co.uk Network: http://uk.linkedin.com/in/gaildonegan Senior Scientist/Team Leader/Research Manager Outgoing and energetic professional with 10 years experience in academic and clinical research. Excellent proven track record of bringing projects to successful conclusion using effective delegation, time management and progress monitoring strategies while networking and identifying opportunities for future growth. Wide-ranging hands-on expertise in regenerative medicine, developmental toxicology, drug development, biomaterials and stem cell biology. Career to date demonstrates a love of challenge and diversity and I now seek higher level scientific management opportunities in the global life sciences arena. Experience Employment Education Selected Publications References and Recommendations Additional Information Subscribe Experience1 of 9 27/09/2011 10:45
  2. 2. Professional Profile http://www.gaildonegan.site90.net/ Project Management Negotiation Networking Collaboration ESC isolation & Bioreactor 3D Tissue Immunohistochemistry maintenance ; design Culture Western & Semi- quantitative MSC isolation & maintenance FACS LSCM & SEM Dotblot SDS PAGE PCR ELISA LC/MS (limited) Back to Top of page Employment Liverpool School of Tropical Medicine, Molecular and Biochemical Parasitology Group, Developmental Toxicology: Senior Postdoctoral Associate; 2009 - 2011(Aug) Effectively kick-started EU project as team leader of workpackage-2 research team with the EU Cordis Artemisinins in Pregnancy (ARTEMIIP) consortium at LSTM. The research is aimed towards unravelling the mechanisms behind heart, skeletal and blood cell defects linked to antimalarials used in areas where, due to parasite resistance, existing therapies are no longer effective. Newer antimalarials currently in development are also being screened for adverse effects to the fetus Our studies together with vital work conducted by the Malaria in Pregnancy (MiP) consortium at LSTM to identify alternative strategies to treat malaria in women of childbearing age will contribute towards WHO recommendations to alleviate the massive global burden of malaria. Research Responsibilities Effectively lead small research team and coordinated multiple optimizations of In vivo, ex vivo & in vitro models for developmental toxicology Successfully identified opportunities and new techniques to elucidate mechanisms behind adverse effects on early populations of hematopoietic stem cells. Micromass culture to monitor early molecular and cellular events in pre -cartilage condensation Planning and assay optimization for genomic and proteomic analyses of extraembryonic and embryonic tissues following drug exposure Molecular (IHC & RT-PCR) investigation of function and potential targets of drug- associated oxidative stress and impacts onf hematopoiesis, cardiogenesis and apoptosis Histology for embryonic chondrogenesis/osteogenesis Manual & automated haematological screening of fetal blood samples derived from in vivo and ex vivo models etc. Research Management Responsibilities Ensuring timely completion of ARTEMIP Work Package 2 milestones and deliverables Successful team leadership to four research staff Key role in planning and implementation of RT-PCR, WB, ELISA, IHC, MS/LCMS, LSCM, haematology Assessment of staff resources needed to achieve project deliverables Coaching and mentoring junior staff, HO licences, organizing external training, Amending project licences to accommodate ARTEMIP requirements Ensuring all activities performed by team comply with GCP, GLP and HO requirements, Production of Standard Operating Procedures (SOPs), Developmental Protocols, Review and Validation documents Data analysis using a range of statistical methods Production of Standard Operating Procedures (SOPs) Development Protocols, Review and Validation Documents Promotion of a safe and positive work environement by demonstrating positive cooperative and friendly attitude towards job responsibilities, line managers, fellow works and visitors while2 of 9 27/09/2011 10:45
  3. 3. Professional Profile http://www.gaildonegan.site90.net/ following all safety guidelines and policies Project Management Responsibilities Communicating to senior management overviews of research outcomes/outputs and their stage of development. Reviewing analyzing and formulation of aspects of research and development proposals e.g. objectives / purposes of project, potential applications, costs, equipment and HR requirements. Proactively dealing with changes in the scope of the project as they arose, developing contingency plans and guidelines to minimize potential risks and crises Development and implementation of methods and procedures for monitoring projects, such as audit documents, score sheets, weekly meetings and planners and review documents to inform management of progress towards milestones and deliverables. Negotiation of research collaborations with academia, industry and healthcare organizations. Negotiation of contracts with major equipment suppliers to support research. Tracking research trends and relevant legislation Networking and collaborations between academia, NHS and pharma (GSK, HLS, CR, Aintree University Hospital, Universities of the Algarve, Leiden, Gothenburg, WHO. Other responsibilities Lecturing (MRC Centre for Drug Safety Science): Preclinical Screening for Embryotoxicity and Teratogenicity Set up of EU FP7 Consortium webpage on LSTM website: Artemisinins in Pregnancy (ARTEMIP) Dissemination of research output at internal and external meetings worldwide and online: California, USA: Preclinical Screening Teratogenicity Of Artemisinin Combination Therapies (ACTs) For Malaria In Pregnancy. Donegan GC, et al. Platform Session III on June 29, 201151st Teratology Society Annual Meeting in California. USA Berlin, Germany, Invitation as expert speaker:Advancements in Preclinical Screening Methods for Artemisinins During Pregnancy. 2010 Donegan GC et al. Informa Life Sciences Inaugural Summit: Preclinical Safety and Efficacy Testing of Biological Products. Informa. Berlin, Germany. December 2 - 4 2010 Edinburgh, Scotland:Parasite to prevention: Advances in the understanding of malaria 20-22 October 2010 Manchester, UK, Invitation as expert speaker:NC3Rs Roadshow CRACK-IT to identify and unlock opportunities for scientific progress and reductions in the use of animals while accelerating translation of research into practice. 6 July 2011. Online TV: Artemisinins in Pregnancy: The Research, The Future for the BBC (online Autumn 2011) Birth Defects Research: Preclinical Screening: Teratogenicity of Artemisinin Combination Therapies (ACTs) for Malaria in Pregnancy Donegan G, et al Birth Defects Research (Part A) 91:327_348 (2011) Malaria Journal: Screening Methods for Artemisinins During Pregnancy. Donegan G et al. Malaria Journal 2010, 9 (Suppl 2): Back to Top of page Covance Drug Development: & NHS Clinical Pathology Laboratories : Clinical Scientist (locum) Covered sick and maternity leave, responsible to head of Clinical Pathology departments at Covance and Aintree University Hospitals. 2008 - 2009 - 1 year Responsibilities Management of workflows in high throughput clinical laboratories Effective use of of NHS and CRO laboratory management systems (LIMS) (ClinySYS, (WinPath),3 of 9 27/09/2011 10:45
  4. 4. Professional Profile http://www.gaildonegan.site90.net/ Clinaxys and FoxPro) to manage and consolidate of large data volumes from multiple, integrated diagnostic analysers Reliable and clear reporting to healthcare professionals including theatre staff, consultants, GPs, clinical research investigators and study directors, sympathetic communication with patients and their families during high-stress situations. Haematological analyses (automated/manual) including whole blood count, white blood cell differential, platelet and reticulocyte analyses, Genotype,molecular analyses,INR and ESR testing Using clinical engineering expertise (MSc & PhD) for maintenance and trouble-shooting of automated clinical diagnostic analysers during analyses of kidney, liver and cardiac function, pO2 and pCO2 etc. in addition to whole blood count, white blood cell differential, platelet and reticulocyte analyses, genotype,molecular analyses,INR and ESR testing Diagnostics for qualitive detection of infectious mononucleosis (glandular fever), sickle cells anaemia and malaria in whole blood, CSF and serum. Preparation of slides for bone marrow and blood film analyses QA & QC weekly & monthly metrology, calibrations for species, sex, pre- existing conditions, NEQAS & EQAS (Addenbrooks Hospital, Cambridge)to ensure regulatory compliance. Back to Top of page University of Bath, Dept of Chemical Engineering / Centre for Regenerative Medicine: Postdoctoral Research Officer. Successful implementation of new methods to produce co- polymeric and hydrogel scaffolds for 3D bone tissue models with better surface characteristics, improved sterility and reproducibility. 2006 - 2007 - 1 year Research responsibilities Successful development of bespoke co- polymer and hydrogel scaffolds for 3D musculoskeletal tissue models. Enhanced annular flow bioreactor design for osteogenic progenitor cell expansion and differentiation on PLGA & PLLA scaffolds Initiated new and simpler methods to produce PLGA micro-tubular tissue culture substrates for improved surface characteristics, sterility and reproducibility Optimized non-invasive cell tracking methodologies on non-transparent biomaterial scaffolds using colloidal staining technologies Adaptation of cell viability assays to permit non-invasive measurement of tissue model viability prior to and following perfusion culture Effective use of IHC & histology SDS PAGE, Western blot, qPCR, cell cycle analyses, ELISA to elucidate impacts of PLGA degradation under flow on MSC expansion Effective liaison and networking with collaborating departments, Universities in the UK and US and hospital personal to facilitate long- and short term project initiatives Effective reporting, data interpretation and consolidation of information from raw datasets Presentation at teaching seminars and regular teleconferences outlining results, projections and future objectives at internal and external meetings Management of tissue culture laboratories, working to and writing SOPs, keeping records of cell source, archiving, disposal etc. Maintenance of databases and spreadsheets to support budget management Purchase and routine maintenance of key analytical equipment, implementing service contracts and payment schedules Other responsibilities Production and presentation of weekly training seminars focused towards bioreactor optimization and bioprocessing technologies, selection, expansion and exploitation of mesenchymal stem cell culture, non-invasive monitoring technologies for continuous tracking4 of 9 27/09/2011 10:45
  5. 5. Professional Profile http://www.gaildonegan.site90.net/ of cellular function and viability Acting as liaison with local schools in Bath and Bristol to promote careers in science Student training and mentoring of tissue culture techniques, bioreactor setup, stem cell culture and maintenance Back to Top of page University of Liverpool, Dept of Clinical Engineering: Postgraduate Researcher/PhD. Doctoral research into vascular cell response to improve tissue engineered blood vessel integrity in response to defined physiologically-relevant flow regimes. 2002 - 2005 - 3 years Research responsibilities Successful design and build of rotating wall bioreactors for tissue culture under physiologically -relevant conditions Generation of functional tissue engineered 3D blood vessel models suitable for pharmacokinetic/pharmacodynamic analysis Development of non-invasive integrated monitoring modalities to measure vascular tissue remodelling response under fluid shear stresses Efficient and reproducible production of vascular smooth muscle cell sheets from donated human tissue for seeding biomaterial scaffolds Innovation of technologies for quantitative uniaxial tensile testing and cell proliferation analyses of vascular graft models. Advanced statistical analyses:Correlation analysis, MANOVA combined with ANCOVA to investigate identifiable correlations between dynamic structural and degradative tissue remodelling under specific flow regimes. Successful utilization of hydroxyproline assays, immuncytochemistry, MMP activity assays, SDS PAGE, Western blot, ELISA, FACS to evaluate impacts of dynamic culture conditions on vascular cell functionality. Strategic collaborations with key university departments, allied medical research charities and biopolymer/pharmaceutical companies. Back to Top of page Education DATE COURSE INSTITUTION FOCUS 2010 Prince 2 Foundation Datrix Project Management and Practitioner 2010 University of Change Management - Models for shaping Managing Change Liverpool and implementing change successfully 2010 University of Developing flexibility of leadership styles to Managing Your Team Liverpool get the most from your team5 of 9 27/09/2011 10:45
  6. 6. Professional Profile http://www.gaildonegan.site90.net/ 2010 Influencing and University of Developing effective communication skills, Negotiation Liverpool Assessing effectiveness of key messages. 2010 Reproduction Huntingdon Life Industry standard developmental toxicology Toxicology Sciences drug screening protocols Faculty of Utilization of Bioreactors 2003 - PhD in Regenerative Medicine and Semi- Synthetic Biomaterials 2005 Medicine University of Liverpool in Tissue Engineered Blood Vessels Faculty of Switching Off Genomic Imprinting 2001 - MSc in Medicine to Induce Cardiomyogenesis in 2003 Clinical Engineering University of Liverpool &n bsp; Mesenchymal Stem Cells 1997 - BSc in School of Biological Sciences University of Investigation of Transcription Factor 2000 Molecuar Biology Liverpool Analogues in Yeast and Mammalian Systems Back to Top of page Selected Publications and Presentations California, USA: Preclinical Screening Teratogenicity Of Artemisinin Combination Therapies (ACTs) For Malaria In Pregnancy. Donegan GC, et al. Platform Session III on June 29, 201151st Teratology Society Annual Meeting in California. USA Berlin, Germany:Advancements in Preclinical Screening Methods for Artemisinins During Pregnancy. 2010 Donegan GC et al. Informa Life Sciences Inaugural Summit: Preclinical Safety and Efficacy Testing of Biological Products. Informa. Berlin, Germany. December 2 - 4 2010 Edinburgh, Scotland:Parasite to prevention: Advances in the understanding of malaria 20-22 October 2010 Manchester, UK:NC3Rs Roadshow CRACK-IT to identify and unlock opportunities for scientific progress and reductions in the use of animals while accelerating translation of research into practice. 6 July 2011. Online TV: Artemisinins in Pregnancy: The Research, The Future for the BBC (online Autumn 2011) Birth Defects Research (Part A): Preclinical Screening: Teratogenicity of Artemisinin Combination Therapies (ACTs) for Malaria in Pregnancy Donegan G, et al Birth Defects Research (Part A) 91:327_348 (2011): Malaria Journal: Adv ancements in Preclinical Screening Methods for Artemisinins During Pregnancy. Donegan G et al. Malaria Journal 2010, 9 (Suppl 2): Journal of Tissue Engineering and Regenerative Medicine: Investigating the Importance of Flow when Utilizing Hyaluronan Scaffolds for Tissue Engineering. 2009 Donegan GC et al. Journal of Tissue6 of 9 27/09/2011 10:45
  7. 7. Professional Profile http://www.gaildonegan.site90.net/ Engineering & Regenerative Medicine. Volume 4, Issue 2, pages 83-95 : Youtube: Malaria: What Where Why & How? How do you catch malaria? What are the stages of the disease? What is multi-drug resistance? What impact does parasite resistance have in the fight against global malaria resurgence? What are antimalarial treatments are available and which should I take them before traveling to malaria- endemic countries? This is one of a series of Youtube videos designed to answer some of these questions A Novel System for Non-Invasive Monitoring of Tissue Remodelling Responses in Vascular Constructs During Dynamic Culture. Donegan GC, et al. In preparation Cell-seeding And Proliferation In The Lumina Of PLLA Tubules In A Dynamic Couette-Taylor Bioreactor, Curran SJ, Donegan GC, et al. 2003 Tissue Engineering, 9(4): 801 The Bioengineered Cardiovascular Construct: Measuring Remodeling Protein Synthesis in Response to Rotational Stresses. Donegan GC, et al. Oral Presentation Joint Meeting of the Tissue Engineering Society International and the European Tissue Engineering Society (TESI-ETES now TERMIS) Lausanne, Switzerland, October 10 - 13 2004 The Effect of Rotational Stresses on Circumferential Remodeling Response in the Tissue Engineered Vascular Construct Donegan GC, et al. Joint Meeting of the Tissue Engineering Society International and the European Tissue Engineering Society (TESI-ETES now TERMIS) Lausanne, Switzerland, October 10 - 13 2004 Back to Top of page Recommendations: "Gail has been responsible for many changes and improvements to this project, expanding and enhancing the research profile at LSTM" Dr. Geoff Edwards, Centre for Drug Safety, University of Liverpool and Liverpool School of Tropical Medicine UK Gail is a very hard worker and a great team player", Dr Stephen Curran, Scheme Manager and Technical Specialist, BSI< /span> "Gail has a friendly, enthusiastic personality, forming good working relationships with her colleagues. It has been a pleasure to work with her" Dr Patrick Doherty, Course Director at Dept of Clinical Engineering and Director of Studies in Centre for Lifelong Learning & Professional Development, University of Liverpool "Gail is a dedicated and innovative scientist. She is also great to work with, combining common sense with a broad knowledge base and excellent problem solving skills. I wouldnt hesitate to recommend her" Dr Muna Whyte, Project Manager, ACM Global Detailed references upon request Back to Top of page7 of 9 27/09/2011 10:45
  8. 8. Professional Profile http://www.gaildonegan.site90.net/ Additional Information Preclinical Study Design:Directly responsible for setting up a range of reprotox models including embryonic (haematopoietic stem cell cultures applicable for developmental toxicology and cancer research, micromass culture for skeletal development and ADME with postimplantation WEC, a reprotox-specific 48 or 72 hour experiment which enables monitoring of mammalian fetal development in the first trimester of pregnancy and bio analysis to characterize adverse drug effects and potential for lethality, birth defects or dysfunction of multiple organ systems. The successful conclusion of which enables the Artemisinins in Pregnancy (ARTEMIP) produce relevant preclinical data to enhance information generated during field trials by the Malaria in Pregnancy (MiP) for the WHO to make accurate risk: benefit assessments as to the deployment of newer antimalarial therapies to women of childbearing age. Protocols and GCP - An acute awareness of the importance of well-designed trials especially when working to tight deadlines. Developed multiple protocols in accordance with GCP and Home Office guidelines. The preclinical studies were meticulously designed to respect rights and dignity at all times and ensure that:- In vivo studies were replaced with ex-vivo and in vitro studies when appropriate and feasible to do so, and for initial screening for lead compounds. Numbers for in vivo experiments kept to a minimum, the maximum information is gained from each series of experiments, and also by re- examining the findings of pre-existing studies Experiments were designed to ensure as little stress as possible Studies were designed so that they did not need to be repeated more than was necessary for validated results using feasibility studies at the outset. Experimental work by new staff was conducted under supervision of an experienced HO licensee until full competency has been ascertained and the worker can be signed off. Feasibility: No trials should be undertaken before a full program of feasibility, risk/hazard assessment, bench testing and validation has been conducted therefore relevant feasibility studies were designed in advance to demonstrate proof of concept, to attempt elucidate unforeseen risks and to determine whether risks are acceptable during early stages. Study management: Involvement in the management of key staff in a number of projects involving multi-disciplinary teams at establishments in the UK, EU and US. Responsible for kick starting the developmental toxicity research from my joining LSTM in 2009 and have lead the small research team to success in this highly relevant and worthwhile project to improve human health. Directly responsible in setting clearly defined long- and short-term project objectives with the agreement of the whole team including senior management. Anticipated delivery dates were also agreed with the potential for failure to achieve objectives taken into consideration, contingency strategies to deal with setbacks as they arise together with initial feasibility studies and risk/hazard studies Ensured that there was continuous and thorough tracking of research outputs throughout with relevant documentation including; SOPs with fully defined objectives and details of the controls against which the collected information will be statistically analyzed. The exact methods should be detailed such that all parties can execute the work in a comparable fashion Toxicology-specific score sheets to track and quantify QA, QC and robustness (reproducibility) and validation8 of 9 27/09/2011 10:45
  9. 9. Professional Profile http://www.gaildonegan.site90.net/ Documented feedback from clients/end users Review of the relevancy of the experimental data in terms of the overall project objectives (deliverables) Possible deviations from the original SOPs and deliverables such as regulatory changes or changes in legislation Tracking failure, cause, recommended action, and outcome Dissemination and Invitation: Invited as an expert speaker in drug development, developmental toxicology and NC3Rs meetings in the UK (Manchester and Edinburg) and abroad (Beijing, Berlin & California) during time at the Liverpool School of Tropical Medicine. Back to Top of page Subscribe RegenerativeMed1 2 videos | 2 subscribers Subscribe Resume created in HTML in May 2011 by Gail Donegan9 of 9 27/09/2011 10:45

×