37. Urine Chemical Exam: pH Bilirubin Protein Occult Blood Glucose Ketones Specific gravity Nitrites WBCs
38. Heartworm Test Buffy Coat method (hematocrit test) Modified Knott’s technique Filter membrane technique/Di-fil Blood Smear SNAP: Antigen testing does not detect the actual parasite but the host’s response to the disease
39. Steps For Modified Knotts Mix 1 ml of blood w/ 9ml of 2% formalin in centrifuge Centrifuge tube at 1300 to 1500 rpm for 5 minutes Pour off liquid supernatant, leaving sediment at bottom of tube Add 2-3 drops of stain to sediment. Using pipette, mix sediment w/stain Place drop of this mixture on glass slide. Apply coverslip. Examine for microfilariae using 10X objective. Filter Membrane Mix 1 ml of blood w/9 ml of lysing solution in 15 ml test tube Place filter membrane in filter holder, close tightly, do not pinch seal in threads of holder Connect syringe to filter holder and gently empty through filter holder Fill syringe with water and repeat above step Open holder, use thumb forceps to remove filter membrane Place filter membrane on a clean microscope slide Add one drop of stain, cover with coverslip, examine for microfilariae
40. Stages of a Parasites Life Cycle 1. oocyst 2. Larvae 3. nymph 4. Pupa 5. Adult
