Nuliv An Overview Of Nu Liv Science 2010 6 21


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Nuliv An Overview Of Nu Liv Science 2010 6 21

  1. 1. Presenting NuLiv Science International July, 2010
  2. 2. NuLiv Operates on 6 Interconnected Business Platforms 1. Focused RESEARCH on METABOLISM (slides 3- 16) 2. Discovery of novel, safe, and BIOACTIVE botanical FRACTIONS & COMPOUNDS (slides 17 - 25) 3. Development of PRODUCTION PROCESS for NuLiv’s proprietary bioactive fractions and compounds (slides 26 – 28) 4. Development of safe and effective SUPPLEMENT products (slides 29) 5. SOURCING and QC of botanical ingredients (slides 30 – 31) 6. Futuristic WELLNESS products and services platform (A NuLiv experimental model) (slides 32)
  3. 3. Focused Research on Metabolism For close to a decade, NuLiv has centered its research around metabolism, the biological functions that sustain life, at cellular and systemic levels 1. Research initiatives (slides 4 – 6) 1.1 Central belief 1.2 Hypothesis 1.3 Strategy 2. Life Science and Clinical Research platforms (slides 7 –14) 2.1 Facility 2.2 Staff & advisors 2.3 Validation platform 2.4 Supporting framework 3. Patents (slide 15) 4. Papers (slide 16)
  4. 4. The Central Belief that laid down the Foundation for Our Research TCM offers the best organized and time tested holistic and systemic health wisdom. NuLiv’s research idea, discovery of novel bioactive ingredients, and development of nutritional products should germinate from this knowledge base
  5. 5. The Hypothesis Our Research Strategy is Built Upon • “Qi” (breathing, life force, energy) and “Xue” (circulation), the foundation of TCM principle, are the metaphysical transformation and final manifestation and distribution of nutrients in human body. • The biological pathway of this manifestation and distribution, i.e., nutrient absorption, transportation of nutrients through bodily fluids (blood included), metabolic and catabolic functions, regulation of haemopoiesis & angiogenic functions, even its effect on immune functions, are all integral to this final manifestation. • “Qi” and “Xue” herbs must be bioactively involved in the various biological pathways and functions. Study of these herbs should offer better odds of novel discoveries and understanding of their benefits toward human health. .
  6. 6. Our Strategy is to Build a Research Platform that Allow us to Discover Novel Bioactive Compounds from Chinese “Qi” and “Xue” Herbs 1. Select safe and widely used TCM herbs as research subjects. 2. Extract, separate, fractionate, and purify these herbs to fraction and/or compound levels. 3. Develop bioassays to identify purity and concentration level of these fractions and compounds. 4. Formulate application specific functional ingredients composed of these fractions and/or compounds and assay for their efficacies. 5. Test ingredient efficacy and safety to the technical or financial level that is feasible; cell, animal and human. 6. Develop and test for ingredient reproducibility and stability.
  7. 7. NuLiv’s Advanced and Integrated Life Science and Clinical Research Platform Component Description Facility NuLiv Taipei Laboratory NuLiv Shanghai Laboratory Management, Staff and Advisors Highly educated and trained in business, multi scientific & clinical disciplines, and work closely in a truly interdisciplinary manner Discovery and Validation In-house and 3rd party in vitro, in vivo, and human use study platforms Supporting Framework/Network Supported by NuLiv’s own subsidiaries and affiliates, and long term strategic partners and alliances in many distinguished academic and research institutions
  8. 8. Research Facilities Taipei and Shanghai Laboratories • NuLiv Taipei (NDMC) Laboratory 1. Research initiatives 2. Research: Chemistry, cell assay, animal and human study on plant derived bioactive fractions/compounds 3. Product design and development 4. Production process development • NuLiv Shanghai Laboratory 1. Extraction, fractionation, purification of botanicals 2. Production process, MOA, and CMC development 3. Sourcing and Procurement 4. QA and QC
  9. 9. Laminar Flow, Cell Culture Water Purification System CO2 Incubator Lab Bench Spectrophotometer Photomicrographic Equipment High Speed Centrifuge PCR machine Gel Dryer Deep Fryer Gel Photosystem Animal Room National Defense Medical Center NuLiv Taipei Laboratory Has Access to Equipments to Perform a Wide Range of In Vitro and In Vivo Studies
  10. 10. Shanghai laboratory Develops Production Process Criteria, Performs QC Extraction/Separation / Isolation Room Chemical Analysis Room Extraction Equipments Waters 2695 HPLC Agilent 1100 HPLC Agilent 6890N/5973 GC/MS Camag TLCS Shimadzu 2401PC UV spectrophotometer Nikon YS2 biological microscope Instrumental Analysis Room
  11. 11. NuLiv Management /Business & Research Executives Founder & CEO Austin Wang, M.S. NuLiv Holdings, Inc. (World Headquarters) Taipei City, Taiwan Executive Director Y.H. Lee, M.D. Vice Chancellor, China Medical Univ. (Taiwan), Previously Dean, Chang Kung Medical School, Dep. Director, Chang Kung Hospital System (10,000+ beds) Principle Scientific Advisor H.C. Lin, Ph.D. Univ. of North Carolina. Taught TCM at NDMC for over 20 years. Pioneered the hypothesis that “Qi” herbs regulate absorption and other metabolic functions President Americas, Europe, Asia, Africa Business Development Sales & Marketing Representative Americas, Europe, Asia, Africa Michael Wang, M.S. NuLiv Science USA, Walnut, CA David Allen, Ph.D. World Wellness, LLC., San Diego, CA President Greater China David Wu, Ph.D. Business Administration, Univ. of South Australia, Director Research & Development NuLiv Taipei Laboratory Thomas Yang, Ph.D., Molecular Microbiology, Univ. of Texas, Southwestern Medical Center Director NuLiv Shanghai Laboratory Sunnie Sun, Ph.D., Pharm. Analysis, Sheng Yang Pharm. Univ. Director Clinical Research Rose Chen, M.D., Ph.D., Shanghai TCM University
  12. 12. NuLiv’s Scientific Advisors Chang, T.C., Ph.D. Biochemistry, Univ. of Illinois, Professor, Dept. of Biochemistry, National Defense Medical Center,Taiwan Chang, W.L., Ph.D. Pharmacy, National Taiwan Univ., Professor, School. of Pharmacy, National Defense Medical Center, Taiwan Kuo, C.H., Ph.D. Physiokinetics, Univ. of Texas, Professor and Chair, Department of Exercise Science, National Taipei Physical Education College, Taiwan Chen, C.X., Professor Director, Department of Pharmacology, TCM Univ., Shanghai, China. Serves as our toxicity and safety advisor on TCM and other herbs Komatsu, Yasuhiro, Ph.D. Medical science, Juntendo University, Prior Dean of R&D department, Tsumura & Co. Chu, C. H, Ph.D. Food engineering, University of Tokyo, expert in pre-clinical pharmacology tests Fan, David, Ph.D. Senior Lecturer, Trinity College, Cambridge Univ., Editor-in-chief, Angiogenesis Hsieh, Steve, Ph.D. Nutritional Biochemistry, Univ. of Wisconsin, Ex Vice Chairman, National Science Council, R.O.C. Government Looi Hwang, Ph.D. Industrial & Environmental Pharmacy Ph.D. from Purdue, previously G.M. of MSD, Hanzhou, China & and Merck of Singapore J.K. Luo, Ph.D. Editor (acting), Journal of Heterocyclic Chemistry, Professor at University of S. Florida Funfack, Wolf, M.D. Metabolic Balance founder. Peng, C.K., Ph.D. Statistical Physics, Boston Univ., Assoc. Professor/Co-director, Rey Institute for Nonlinear Dynamics in Physiology and Medicine, Harvard Medical School.
  13. 13. Our In vitro, In vivo, and Human Research Platforms In vitro Assay Systems • Establish any cell assay system in 2 to 4 months • Currently available cell assay systems: HaCaT and HDF cell, Caco-2, 3T3-L1, HSMMT,HepG2, HCH & SW 1353, and etc In vivo Assay for efficacy and safety Systems • Nude mice for cancer and cosmetic studies • db/db mice for diabetes and wound healing studies • SD rat for ergogenic and diabetes studies • absorption model (portal vein) • Acute and Sub-acute Toxicity Human use Study • Topical skin care test • Ergogenic test • Cancer adjuvant therapy
  14. 14. Our Supporting Framework & Network Facility, equipment, interdisciplinary consultation National Defense Medical Center Industrial Technology Research institute, Taiwan Yang Ming University, Taiwan Taipei Medical University, Taiwan In vivo and human study China Pharmaceutical University, Nanjing, China Taipei Physical Education College I-Shou University Hospital NuLiv Wellness Clinic Consultation, Cellular science Metabolic health TCM pharmacology & toxicology angiogenesis Yang Ming Univ. Stem Cell Research Center Taipei Medical Univ. Stem Cell Research Center Dr. Wolfgang Funfack, Metabolic Balance, De. Shanghai TCM university China Medical University, Taiwan Trinity college, Cambridge University, UK
  15. 15. Title Country/Region Patent NO. Application NO. Method for Regulating Nutrient Absorption with Astragalosides USA US11/426,029 China CN200610090113.0 Taiwan I271195 Method for Regulating Nutrient Absorption with Ginsenosides USA US11/426,06 China CN200610090114.5 CN200910178493.7 CN200910178492.2 Taiwan I317280 Method for Skin Care PCT PCT/US07/77326 USA US11/469,659 China CN200710089586.3 Taiwan TW095132614 Use of Cycloartane compounds for treating arthritis USA US12/353,779 China N/A yet Taiwan TW098135306 Patents from NuLiv’s Research
  16. 16. • Effect of ginsenosides on glucose uptake in human Caco-2 cells is mediated through altered Na+/glucose cotransporter 1 expression. Chang TC, Huang SF, Yang TC, Chan FN, Lin HC, Chang WL. J Agric Food Chem. 2007 ;55(5):1993-8. • Effect and mechanism of ginsenosides CK and Rg1 on stimulation of glucose uptake in 3T3-L1 adipocytes. Huang YC, Lin CY, Huang SF, Lin HC, Chang WL, Chang TC. J Agric Food Chem. 2010 ;58(10):6039-47. • Glycogen overload by postexercise insulin administration abolished the exercise-induced increase in GLUT4 protein. Chou CH, Tsai YL, Hou CW, Lee HH, Chang WH, Lin TW, Hsu TH, Huang YJ, Kuo CH. J Biomed Sci. 2005 ;12(6):991-8. • The induction of orphan nuclear receptor Nur77 expression by n-butylenephthalide as pharmaceuticals on hepatocellular carcinoma cell therapy. Chen YL, Jian MH, Lin CC, Kang JC, Chen SP, Lin PC, Hung PJ, Chen JR, Chang WL, Lin SZ, Harn HJ.Mol Pharmacol. 2008 ;74(4):1046-58. • The natural compound n-butylidenephthalide derived from Angelica sinensis inhibits malignant brain tumor growth in vitro and in vivo. Tsai NM, Chen YL, Lee CC, Lin PC, Cheng YL, Chang WL, Lin SZ, Harn HJ. J Neurochem. 2006;99(4):1251-62. • The antitumor effects of Angelica sinensis on malignant brain tumors in vitro and in vivo. Tsai NM, Lin SZ, Lee CC, Chen SP, Su HC, Chang WL, Harn HJ. Clin Cancer Res. 2005;11(9):3475-84. • Acetone extract of Angelica sinensis inhibits proliferation of human cancer cells via inducing cell cycle arrest and apoptosis. Cheng YL, Chang WL, Lee SC, Liu YG, Chen CJ, Lin SZ, Tsai NM, Yu DS, Yen CY, Harn HJ. Life Sci. 2004;75(13):1579-94. • Ginsenoside Rb1 inhibits tube-like structure formation of endothelial cells by regulating pigment epithelium-derived factor through the oestrogen beta receptor. • Leung KW, Cheung LW, Pon YL, Wong RN, Mak NK, Fan TP, Au SC, Tombran-Tink J, Wong AS. Br J Pharmacol. 2007;152(2):207-15. • Metabolites of ginsenosides as novel BCRP inhibitors. Jin J, Shahi S, Kang HK, van Veen HW, Fan TP. Biochem Biophys Res Commun. 2006;345(4):1308-14. • Signaling pathway of ginsenoside-Rg1 leading to nitric oxide production in endothelial cells. Leung KW, Cheng YK, Mak NK, Chan KK, Fan TP, Wong RN.FEBS Lett. 2006 ;580(13):3211-6 • Modulating angiogenesis: the yin and the yang in ginseng. Sengupta S, Toh SA, Sellers LA, Skepper JN, Koolwijk P, Leung HW, Yeung HW, Wong RN, Sasisekharan R, Fan TP. Circulation. 2004;110(10):1219-25. • S.-F. Huang, Y.-C. Huang, J.-Y. Lin, W.-L. Chang and T.-C. Chang (2010) Effect of Ginsenoside Rb1 on Glucose Uptake and Expression of Glucose Transporters in Animal Cells, Adaptation Biology and Medicine, Volume 6: Cell Adaptations and Challenges, 2010 (In Press). • Yu-Chuan Huang, Cheng-Yu Lin, Su-Fen Huang, Wen-Liang Chang, and Tsu-Chung Chang (2010) Differential effect of oleanolic and ursolic acid on GLUT4 expression and membrane translocation in 3T3-L1 adipocytes, Manuscript in preparation. Published Papers and papers in Press
  17. 17. Discovery of Novel, Safe, and Bioactive Botanical Fractions and Compounds After a decade of following our research central belief, hypothesis, and strategy, and by subjecting dozen “Qi” and “Xue” herbs through our in vitro, in vivo, and human studies, we have discovered 6 bioactive fractions and compounds in these herbs that have shown dose-dependent benefit toward specific human functions 1. 6 bioactive fractions / compounds discovered (slide 18) 2. Functional ingredients formulated with our bioactive fractions/compounds 2.1 Brief descriptions of the 6 functional ingredients (slide 19) 2.2 ActiGinTM , for optimum exercise energy, endurance, and recovery (slide 20) 2.3 AstraGinTM , for enhanced nutrients absorption at the cellular level (slide 21) 2,4 AstrionTM , for youthful and radiant skin (slide 22) 2.5 ImmunoAGTM , for adjuvant therapy (slide 23) 2.6 OsteoSineTM , for reversing bone loss (slide 24) 2.7 Slim&FitTM , for weight control and metabolic syndrome (slide 25)
  18. 18. 6 Fractions / Compounds from our Research and Their Biological Functions Fractions/Compounds Discovered Functions NL-F001 Anti-angiogeneis NL-F002 Enhance amino acids, vitamins, and glucose absorption at the cellular level; anti-angiogenesis NL-F003 Increase Adiponectin secretion Ergogenic/recovery NL-F004 Enhance amino acids, vitamins, and glucose absorption at the cellular level NL-F006 Enhance amino acids, vitamins, and glucose absorption at the cellular level ; Increase Adiponectin secretion NL-F007 Increase collagen and hyaluronic acid secretion; decrease MMP-1 secretion
  19. 19. Proprietary Nutraceutical Ingredients Formulated with NuLiv Bioactive Fractions and Compounds AstraGinTM An all natural nutrient uptake enhancing complex that improves amino acids, glucose, vitamins absorption by 62%, 57%, 50% through regulation and parallel alteration on both the protein and mRNA expression levels of the nutrient transporter genes AstrionTM Reduces the number and appearance of fine lines & wrinkles, skin discoloration due to sun damage, and firms, tones, and hydrates skin through increased dermal genesis and UV protection. Dermal genesis promotes the synthesis of new collagen and hyaluronic acid and, at the same time, reduces the breakdown of existing collagen due to UV light destruction ActiGinTM Increased delivery of glucose to muscle cells; Protect against free radical attack after exercise; Anti-inflammation; Minimize muscle cell damage; Increased glycogen replenishment after Intensive workout ImmunoAGTM ImmunoAGTM promotes nutrient absorption and metabolism in intestinal cells, Inhibits abnormal cell proliferation and angiogenesis, induces abnormal cell mitotic arrest and apoptosis through the activation of multiple signal pathways. In an in vivo study, ImmunoAGTM demonstrated to inhibit abnormal cell growth by 44% and prolonged the life of subcutaneous abnormal cell bearing rats by 33% when compared to a control group. OsteoSineTM Promotes bone mineral density and structural strength by enhancing bone formation and reducing bone resorption. This dual action has been confirmed by NuLiv’s PO (post- ovariectomized) female rats (equivalent to menopausal women) and RA (retinoic Acid- induced acute osteoporosis) rats (Equivalent to none gender or age-related osteoporosis persons) models. Slim&FitTM Decreases the intestinal absorption of glucose through the regulation and parallel alteration on both the protein and mRNA expression levels of the nutrient transporter genes while increases the glucose absorption in muscle and adipocyte cells through insulin independent pathway.
  20. 20. Creatine kinase MDA IL-6 Inflammatory biomarkers inhibition Exercise Oxidative stress elevation Glucose uptake Oxidative stress reduction ATP How ActiGinTM Works
  21. 21. How AstraGinTM Works AstraGinTM ’s Nutrients Absorption Model
  22. 22. How AstrionTM Works
  23. 23. Pharmacodynamics of ImmunoAGTM
  24. 24. OsteoSineTM In vivo Study Normal bone treated with retinoic acid to simulate osteoporosis conditions Treated with 400 mg of OsteoSine daily for 5 weeks
  25. 25. How Slim&FitTM Works Slim&Fit TM ’s Biological Pathways/Mechanisms on Regulating Metabolic Functions
  26. 26. Development of Production Process for NuLiv’s Proprietary Bioactive Fractions and Compounds We develop production process, reference standard, MOA, bioassay, and QA Programs for the production of our proprietary active fractions and compounds so that they have the same purity and concentration level as in those used in our research. 1. Extraction, Fractionation, Separation, and Purification of Raw Herbs to the Fraction and Compound Levels (slide 27) 2. Purity and concentration level of our proprietary bioactive fractions / compounds are identified and measured by specific bioassays (slide 28)
  27. 27. Extraction, Fractionation, Separation, and Purification of Raw Herbs to Fraction and Compound Levels
  28. 28. The Purity and Concentration Level of our Bioactive Fractions and Compounds are Identified and Measured by Specific Bioassays Active constituentsMedicinal plants Crude extracts Fraction 1 Fraction 2 Fraction 3 Fraction 4 Fraction 5 Fraction 6 … fractionationextraction Formulation Depend on function Products & Marketing Structure elucidation UV MS NMR IR CD 1 H NMR 13 C NMR 2D NMR separation Absorption Anti-cancer Skin care, etc Active constituents QC systems -LC/UV -GC/MS Bioassay QC
  29. 29. Our Supplements are Highly Effective and Safe in Supporting Critical Human Health Functions
  30. 30. Our Sourcing Operations Guarantees Premier Quality Products with Competitive Prices and Timely Delivery to our Customers NuLiv/Customer specifications/requirements Vendor Vendor Survey for Certificates, Qualifications, MOA, COA, sample Onsite Vendor Survey Disqualified Qualified Contract/Agreement Primary suppliers Secondary Supplier
  31. 31. Our Quality Control on Sourcing Operations Closely Follows Pharmaceutical Protocols • Follow USP/NF, AOAC, ChP, GB, In-house developed MOA • PhytoMonitorTM Double QC Checking – First tested at the manufacturing sites or production sample tested at NuLiv shanghai Laboratory – Production order samples tested at NuLiv Shanghai laboratory before order can be shipped. – Samples can be tested by independent 3rd party laboratories in the U.S. for additional quality control. – Alkemists Pharmaceuticals, CA – Michelson Lab, CA – Oregon Department of Agriculture (heavy metals)
  32. 32. Futuristic Wellness Product and Service Platform (A NuLiv Experimental Model) Wellness Advisors Interactive and personal wellness information exchange and virtual community management NuLiv Wellness Management Inc. Device HRM Meridian Blood analysis CD/Biochips Nutritional Sciences NuLiv Laboratories (Slides 1 – 42) System complexity analysis DynaDx – Harvard Reylab Evidence based wellness know-how NuLiv Wellness Clinic Wellness/Sub-clinical Primary Care Clinic Cellular sciences Steminent Therapeutic Inc.
  33. 33. Whether its research capability, cutting edge proprietary botanical fractions, highly effective nutritional supplement products, premium quality botanical ingredients, or even Futuristic Wellness Model, NuLiv Science is the company that you can trust. Thank you