Culture media

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Culture media

  1. 1. Made by :Group# C1Fasahat Ahmed Butt (36)
  2. 2.  A culture medium is a compound , which hasconstituents that are essential for life ,multiplication and growth of micro-organisms.1) Nutrients2) Mineral salts3) suitable pH4)Accessory growth factors
  3. 3. (A) LIQUID MEDIUM:1) Peptone water2) Nutrient Broth(B) SOLID MEDIUM:1) Nutrient agar
  4. 4.  Simple media Enriched media Differential media Selective media Transport media
  5. 5.  Consists of only basic substances such as,nitrogen , carbon , minerals. EXAMPLES: Nutrient broth Nutrient agar Peptone water
  6. 6.  Some enriched material like blood and serum isadded to the medium , required for propergrowth of some bacteria EXAMPLES: Blood agar Chocolate agar
  7. 7.  This type of media is used to differentiatebetween two groups of bacteria . EXAMPLES: MacConkey’s medium
  8. 8.  An inhibitory substance is added to the mediawhich prevents growth of all organisms exceptthe one for which it is designed. EXAMPLES: Loweistein jensen’smedium
  9. 9.  A media used to transport specimen to keepthe organism alive EXAMPLES: AMIE’s medium
  10. 10. HOW TO IDENTIFYBACTERIA’S USINGCULTURE MEDIA ????
  11. 11.  MICRO-ORGANISMS CULTURED: Staph.aureus golden yellow colonies Staph.citrus lemon yellow colonies Pseudomonas green colonies
  12. 12.  MICRO-ORGANISMS CULTURED: Complete (beta) hemolysis: Strep.pyogenes In complete (alpha) hemolysis: Streptococcus pnuemonia Non-hemolytic :staph.aureus and staph.citrus
  13. 13.  MICRO-ORGANISMS CULTURED:1)H.influenza2)Neisseria.meningitidis
  14. 14.  MICRO-ORGANISMS CULTURED: 1) lactose fermenters (pink colonies):E.coli. 2) non-lactose fermenters (colour less colonies):shigella
  15. 15.  MICRO-ORGANISMS CULTURED: Mycobacterium tuberculosis

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