EuFMD OS20

1. ACADEMIC RESEARCH POSTER TEMPLATE
Subtitle for Academic Research Poster (48x36 inches)
Your names and the names of the people who contributed to this presentation
Introduction
In Republic of Korea, three commercial foot-
and-mouth disease (FMD) vaccines containing
different FMD virus (FMDV) strains (A vaccine:
O1 Manisa + O 3039 + A22, B vaccine: O
Primorsky 14 + A Zabaikalsky 13, and C vaccine:
O1 Campos + A2001 Argentina + A24 Cruzeiro
vaccine) were allowed for routine vaccination.
Because these vaccines were sometimes
provided to livestock farms randomly from the
government, different types of vaccines could
be used for booster vaccination. Therefore, in
field, there has been a claim that heterologous
booster vaccination could hamper the proper
immune response which should be elicited by
homologous booster vaccination, 2-shot
vaccination with the same vaccine in pigs.
In this study, the serological cross-reactivity
induced by heterologous- and homologous-
booster-vaccination was analyzed by virus-
neutralizing test (VNT) using vaccine strains..
J. Choi1, S. Jung1, S. Lee1, H-H. Kim1, J-H. Park1, and J. Kim*1
THE CHANGES OF SEROLOGICAL CROSS-REACTIVITY BETWEEN
HOMOLOGOUS AND HETEROLOGOUS BOOSTER FOOT-AND-
MOUTH DISEASE VACCINATION IN PIGS
1Center for FMD Vaccine Research, Animal and Plant Quarantine Agency, Gimcheon, Korea
Discussion
Any interference induced by the booster
vaccine was not observed in six heterologous-
vaccinated-groups (Fig. 1). The immune
response against the vaccine strains induced by
booster vaccination in heterologous-booster-
vaccinated groups seemed to be equal or more
than homologous-booster-vaccinated groups at
24 weeks of age.
Reference
[1] De Clercq K et al. 1989. Vet Res Commun.
13:199-204.
[2] Khalifa ME et al. 2017. Vet Microbiol. 210:1-7.
Results
An effective vaccine usually requires more
than one time vaccination according to the
prime-boost platform. Traditionally the same
FMD vaccine are given multiple times as
homologous boost. However, our result
suggested that the heterologous booster FMD
vaccination could induce the proper immune
response. On the other hand, the heterologous
booster FMD vaccination could be used as the
strategic method preventing against a broader
spectrum of field FMD viruses.
Materials and methods
One hundred ninety-eight pigs were randomly
divided into nine groups, which constitute six
heterologous-booster-vaccinated groups (A+B,
A+C, B+A, B+C, C+A, and C+B vaccinated groups,
according to the sequence of applied vaccines)
and three homologous-booster-vaccinated
groups (A+A, B+B, and C+C). After vaccinated at
8 and 12 weeks old with designated vaccines,
VNT against the vaccine strains were performed
until 24 weeks old.
Fig. 1 Humoral immune response induced by heterologous- and homologous-booster-vaccination. (A)
Mean VN titers of sera from pigs primed with A vaccine. (B) Mean VN titers of sera from pigs primed
with B vaccine. (C) Mean VN titers of sera from pigs primed with C vaccine.
Furthermore, the increasing tendency of the
cross-reactivity against other vaccine strains not
contained in both primary and booster vaccines
was more often observed in heterologous-
booster-vaccinated groups than in the
homologous-vaccinated groups.
A
B
C