1.
www.pirbright.ac.ukThe Pirbright Institute receives strategic funding from BBSRC.
Do commercially available lysis
buffers inactivate foot-and-mouth
disease virus?
Britta Wood, Valérie Mioulet, Lissie Henry, Barsha Thapa, Ashley Gray, Don King

2.
Given strict biosafety concerns and regulations for FMDV:
• Critical that lysis buffers used effectively inactivate
• Important for:
- Location of nucleic acid extraction experiments
- External transfer/shipment of samples
Biosafety concern
Purpose: Determine whether commercially available lysis
buffers are able to lyse/inactivate FMDV effectively, as
indicated by the lack of cytopathic effect (CPE)/virus replication
in cell culture.

3.
Lysis buffers:
MagMAX lysis binding solution (Ambion, Life Tech)
MagVet NM1 lysis buffer (LSI, Life Tech)
RNeasy RLT buffer (Qiagen)
Viruses:
All seven serotypes
Cell culture & original suspension
6.9-8.5 log TCID50/mL
Cells:
Primary bovine thyroid (BTY)
Continuous pig (IB-RS-2)
Experimental design
Neg
control
CPE

4.
Results to-date
Lysis buffers inactivate FMDV in cell culture
supernatants and original tissue suspensions.
Future work will be conducted for:
• SVDV
• Different sample types