1.
www.pirbright.ac.ukThe Pirbright Institute receives strategic funding from BBSRC.
Antigen-detection ELISA
performance versus
virus evolution
V.Mioulet, A.Morris, B.Wood, B.Thapa, A.Gray, L.Henry, N.J.Knowles,
J.Wadsworth, D.P.King

2.
WHY?
v Serotyping done using pAg-ELISA
v In the last few years this test failed to serotype a number of samples
v Is there a pattern?
v Samples logged and sets identified
O
A
C
SAT 1
SAT 2
SAT 3
ASIA 1
SVDV
Ctrls

3.
EXAMPLE: O/CATHAY
%
No issues in serotyping using polyclonal
Ag-ELISA
Low to borderline OD values on standard
Ag-ELISA / Confirmation may have been
required using the integrin-mAb ELISA
Additional ELISA required for determination
of serotype

4.
v Best practice to not rely on a single test for serotyping
v Tests in use should be subjected to ongoing validation
against circulating field strains
v Highlights the importance of ongoing work to develop
and validate new assays and reagents to detect
current circulating strains of FMDV
CONCLUSIONS