1.
The challenges of
using in-vitro tests
for Vaccine Matching
(2015-2016)
Abdelghani (Abid) Bin-Tarif |
Bob J. Statham | Anna B. Ludi | Donald P. King
FMD Reference Laboratory

2.
The Pirbright Institute is the OIE, FAO and EU Reference Laboratory for foot-and-mouth
disease (FMD). The Serum Assay Unit carries out vaccine matching tests for international
samples received by the World Reference Laboratory (WRL), to characterise the
antigenic relationship (r1-value) between circulating field strains and vaccine.
Introduction
Samples processing steps

3.
• More than 195 samples submitted to WRL-FMD between submitted
between Jan.2015-Oct 2016 from 27 different countries.
Samples submitted to WRL-FMD
2015-2016

4.
Serotype A:
33 field isolates have been tested by
VNT from five different pools, results
show good match with the vaccine
strains in pools 1 and 5, but show poor
match in pools 3, 4 and 6.The new
lineage G-VII in pool 1 has a poor match
against all vaccine viruses available in
the SAU.
Serotype O:
60 field isolates have been tested by
VNT from five different pools. Results
show good match with the vaccine
strains in all pools.
Results

5.
Future
In the future the WRL along with international reference laboratories
of the OIE/FAO Network is working to harmonise and improve the
robustness and repeatability of the vaccine-matching test.
Acknowledgements
 Don King, Anna Ludi, Bob Statham, Ginette Wilsden and Clare Browning
 Central Service Unit (CSU)
 World Reference Lab (WRL)
 You for submitting samples
 Vaccine companies for supplying reagents
and EuFMD for allowing me to present
Many Thanks .