1.
European Commission for the Control
of Foot-and-Mouth Disease
Open Session of the EuFMD - Cascais –Portugal 26-28 October 2016
Evalua&ng the survival of Foot and Mouth
Disease Virus in the environment
Emma Brown
Research assistant, The Pirbright Ins2tute

2.
European Commission for the Control
of Foot-and-Mouth Disease
Study outline:
q 4x ca>le transmission experiments to study environmental contamina&on and virus
survival
q Environmental sampling to es&mate the decrease in environment infec&ousness over
&me
q Development of environmental sampling methods for surveillance

3.
European Commission for the Control
of Foot-and-Mouth Disease
OS16
Transmission studies #1 & 2: Experimental plan

4.
European Commission for the Control
of Foot-and-Mouth Disease
OS16
Transmission studies #1 & 2: Sampling plan
Samples collected:
q 2 Wall swabs (50x50 area)
q 5 Faecal samples
q 5 Floor swabs (10x10 area)
q 1 Food trough swab
Temperature: 19°C
Rela&ve humidity: 60%

5.
European Commission for the Control
of Foot-and-Mouth Disease
OS16
Transmission studies #1 & 2: Results- Room housing direct contact donors
0
1
2
3
1 2 3 4 5 6
Log10 PFU/ml
Day of study
Trough
Wall
Floor
Faeces
Study 1 Study 2
0
0.5
1
1.5
2
2.5
3
1 2 3 4 5 6
Log10 PFU/ml
Day of study
Floor
Wall
Trough
Faeces
N.B. Day 1 is Day 9 of the transmission studies

6.
European Commission for the Control
of Foot-and-Mouth Disease
OS16
Transmission studies #1 & 2: Results- Room housing inoculated donors
0
0.5
1
1.5
2
2.5
3
1 2 3 4 5 6 7
Log10 PFU/ml
Day of study
Floor
Wall
Trough
Faeces
Study 2
N.B. Day 1 is Day 5 of the transmission study

7.
European Commission for the Control
of Foot-and-Mouth Disease
OS16
Transmission studies #3 & 4: Experimental plan / Sampling plan
AlteraRons to the sampling plan:
q 5 Wall Swabs (10x10cm)
q 2 Food trough swabs

8.
European Commission for the Control
of Foot-and-Mouth Disease
OS16
Transmission studies #3 & 4: Results: room housing inoculated donors
0.0
0.5
1.0
1.5
2.0
2.5
3.0
3.5
4.0
4.5
1 2 3 4 5
log10 PFU/ml
Day of study
Trough
Wall
Floor
Faeces
Study 3
0.0
0.5
1.0
1.5
2.0
2.5
3.0
1 2 3 4 5
log10 PFU/ml
Day of study
Trough
Wall
Floor
Faeces
Study 4
N.B. Day 1 is Day 6 of the transmission studies

9.
European Commission for the Control
of Foot-and-Mouth Disease
OS16
Transmission studies #3 & 4: Results: room housing direct contact donors (Pre-clinical)
0.0
0.5
1.0
1.5
2.0
2.5
3.0
1 2 3 4 5 6
log10 PFU/ml
Day of study
Trough
Wall
Floor
Faeces
Study 3
0.0
0.2
0.4
0.6
0.8
1.0
1.2
1.4
1.6
1.8
2.0
1 2 3 4 5 6
log10 PFU/ml
Day of study
Trough
Wall
Floor
Faeces
Study 4
N.B. Day 1 is Day 8 of the transmission studies

10.
European Commission for the Control
of Foot-and-Mouth Disease
OS16
Transmission studies #3 & 4: Results: room housing direct contact donors (Clinical)
0.0
0.5
1.0
1.5
2.0
2.5
1 2 3 4 5 6 7
log10 PFU/ml
Day of study
Trough
Wall
Floor
Faeces
0.0
0.5
1.0
1.5
2.0
2.5
3.0
1 2 3 4 5 6 7
log10 PFU/ml
Day of study
Trough
Wall
Floor
Faeces
Study 3 Study 4
N.B. Day 1 is Day 10 of the transmission
study
N.B. Day 1 is Day 11 of the transmission
study

11.
European Commission for the Control
of Foot-and-Mouth Disease
OS16
Half lives

12.
European Commission for the Control
of Foot-and-Mouth Disease
OS16
Conclusions
q Live virus can be detected up to 7 days aVer room has been evacuated.
q On average live virus was detected highest and longest in faeces.
q On average the lowest virus was detected on the walls and shortest on the food trough.
q There was not a large difference between virus Rtre and survival Rme between the rooms
contaminated by clinical emission compared to pre-clinical emissions.
q The results of this study will be combined with those of the risk of transmission from
exposure to contaminated environment. This will give a beYer understanding of the effect of
disinfecRon and waiRng Rme before re-populaRon on the control of FMD outbreaks.

13.
European Commission for the Control
of Foot-and-Mouth Disease
OS16
Acknowledgment
s
The Pirbright Ins&tute:
Dr Claire ColenuY
Dr Simon Gubbins
Mr Noel Nelson
Wageningen Bioveterinary Research (WBR) (formally CVI):
Dr Jose Gonzales
Dr Phaedra Eblé
Dr Aldo Dekker
A special thanks to all the animal staff at both insRtutes