Undergraduate Research Conference Funding: Honors Independent Study Project:Carbohydrate Allocation Patterns in Southern Minnesota Herbicide (Aquathol-K)treated Curlyleaf Pondweed (Potomogeton crispus) Populations.My name is Erika Magnusson and I am currently a senior and a declared plant sciencemajor at Minnesota State University Mankato. I have broadened my research in the fieldof plant science by establishing an independent study of undergraduate research under thedirection of MNSU-Mankato Biology department professor Dr. Ruhland and graduatestudent Zach Gutknecht. I will be assisting Zach with laboratory measurements of floraland fauna in order to determine how the application of an aquatic herbicide affects thecomposition and diversity of fauna and flora in a shallow lake ecosystem.Significance of the Research Project: My main objective for undergraduate researchwill be aiding Zach in carbohydrate allocation measurements of Curlyleaf Pondweed(Potomogeton crispus). Potomogeton crispus is the most widely dispersed nuisanceforming non-native submersed aquatic plant in the state of Minnesota (Woolf & Madsen;2003). Potomogeton crispus is considered invasive due to its ability to reproduceprimarily vegetatively (asexually) by the formation of turions, which have a very highgermination rate and a strong rhizome anchoring system. Both of the latter causingPotomogeton crispus’ dense growth to outcompete native aquatic vegetation, degradewater quality, and cause problems for boater’s navigation and recreation. It is evident thatPotomogeton crispus’ has the ability to outcompete and eventually dominate nativevegetation in Southern Minnesota lakes. A university’s biological science department,such as Minnesota State University Mankato located in Southern Minnesota, should beaware of and able to place emphasis on invasive species in its own vicinity.
Proposed Methodology for Attaining Project Goals: My main responsibilities will beto assist Zach in the lab by using a procedure called “Colorimetric Methods forDetermination of Sugars” previously cited by Michel Dubois, K.A. Gilles, J.K. Hamilton,P.A. Rebers and Fred Smith (1955) to determine carbohydrate allocation patterns inherbicide/non-herbicide treated Potomogeton crispus turions. The procedure involves, butis definitely not limited to extracting sugars from weighted Potomogeton crispus turions,hydrolyzing starch to glucose and eventually measure glucose hydrolysate (allocatedcarbohydrate) of each Potomogeton crispus turion.Zach has previously spent much of his time in years 2011-2012 collecting turions ofPotomogeton crispus for carbohydrate analysis. The turions are very small in size, yeteach turion provides valuable information for a database of overall carbohydrateallocation patterns either in response to different light conditions or herbicide treatments.It is very important that each turion is subject to the “Colorimetric Methods forDetermination of Sugars” procedure, which can only occur with proper quantities ofreagents required. Currently, there is a need for more specific enzymes (notably PGO andalpha-amylase) in order to complete to create a proper reagent quantity for further turionanalysis.Anticipated Project Outcomes:Assist Zach with measurements for his comparison of herbicide/non-herbicidetreated floral (turions) in shallow lake ecosystems.Use the Potomogeton crispus carbohydrate allocation measurements to create anUndergraduate Research Symposium presentation of how Potomogeton crispusturion sustainability to store carbohydrates differs after consecutive annualherbicide treatments compared to no treatment at all or one annual treatment.
Overall, this undergraduate research should give not only Southern Minnesota moreknowledge about how turions are affecting its native vegetation, but also give the DNR(Department of Natural Resources) knowledge of how the current herbicide treatmentsare affecting turion carbohydrate allocations. The DNR can then use the turion data toeither look for new herbicide treatments, decrease/increase consecutive yearly herbicidetreatments or look for alternative methods of limiting Potomogeton crispus. The globalperspective of the undergraduate research data of turion samples carbohydrate allocationpatterns should in turn provide better water quality in Southern Minnesota with lessdegradation from invasive Potomogeton crispus or less unnecessary herbicide use by theDNR.Timeline:Start Date 9/1/12TurionScrap Sample Analysis and Experimentation 9/28/12Turion Actual Samples Carbohydrate Allocation Data Collected 10/15/12Different Lake Turion Samples tested 2/1/13Present Information at URC 4/11/13In order to fulfill the requirements for the undergraduate research I am involved with,Carbohydrate Allocation Patterns of Potomogeton crispus Populations, grant money isneeded for the purchase of enzymes. There are four enzymes (1 quantity of each enzyme)needed to complete the research: The exact prices and content are listed as follows:Enzyme Content neededPrice for entirecontentα-Amylase (B. licheniformis) 40mL - 3000 Units/mL $152.21Amyloglucosidase from Aspergillus niger50 mL, aqueoussolution $76.30o-Dianisidine dihydrochloride 5g $43.50PGO Enzyme Preparation1 G capsules-10capsules $92.70Total: $364.71
BibliographyWoolf, T. E., & Madsen, J. D. (2003, June 25). Seasonal biomass and carbohydrateallocation patterns in southern Minnesota curlyleaf pondweed populations. J.Aquat. Plant Manage. 41:113-118.Dubois, Michel, K.A. Gilles, J.K. Hamilton, P.A. Rebers and Fred Smith. 1955.Colorimetric method for determination of sugars and related substances.Analytical Chemistry 28: 350-356.