UPM toxicity of TMOF

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UPM toxicity of TMOF

  1. 1. FINAL REPORTTOXICITY OF TMOF AGAINST THE OIL PALM POLLINATOR, Elaeidobius kamerunicus FAUST Dated 6 January 2011 Prepared by Prof. Dr. Dzolkhifli Omar Department of Plant Protection, Faculty of Agriculture Universiti Putra Malaysia 43400 UPM Serdang Selangor To EntoGenex Industries Sdn Bhd Suite 16-04 & 17-04 Lvel 16 & 17, GTower 199 Jalan Tun Razak 50400 Kuala Lumpur
  2. 2. Toxicity of TMOF against the oil palm pollinator, Elaeidobius kamerunicus Faust IntroductionTMOF is a new insecticide for the control of mosquito. It has been classified as the safe andenvironmental friendly. However, the effect of TMOF on the oil palm pollinator, Elaeidobiuskamerunicus has not been documented. The objective of the study was to evaluate thetoxicity of TMOF against the pollinator. Materials and MethodThe study was conducted in the Toxicology Laboratory, Department of Plant Protection,Universiti Putra Malaysia by exposing the adult pollinator to the residual film of TMOF onglass. The TMOF and its WP TMOF and Rice Husk TMOF formulation were used in the study.The dilutions at the field recommended rate of 0.21, 1.05 and 4.2 g/L of TMOF, WP TMOFand Rice Husk TMOF respectively were prepared using distilled water. A total of 157 uL ofthe dilution was applied to the 9 cm Petri dish and left to air dry. Then, a minimum of 20 tomaximum of 25 adults were placed in the treated glass Petri dish. A cotton wool soakedwith 5% honey solution was supplied to the adults. There were five replicates performulation with one control replicate. The mortality was recorded at 24, 48 and 72 h afterplacement.Control mortality if any was corrected in treatments using Abbott’s formula (Abbott, 1925).The data were transformed to Arcsin and subjected to the Analysis of Variance (SAS) and themeans separation by Tukey’s Test (P = 0.05). Results and DiscussionThe effect of TMOF and its formulations on E. kamerunicus is shown in Table 1. Nomortality was observed in the control. The F values at 24, 48 and 72 h were 1.93, 1.56 and1.53 respectively indicating there was no significant different between the treatments (P =0.05).Table 1. Toxicity of TMOF against E. kamerunicus following exposure at the recommendedrates. Formulation Percentage Mortality ± Std Dev 24 h 48 h 72 h TMOF 5.91± 8.33 6.93 ± 9.49 10.21 ± 9.34 WP TMOF 11.08 ± 10.33 13.39 ± 8.33 19.65 ± 4.87 RH TMOF 13.41 ± 2.90 18.11 ± 5.63 19.07 ± 4.46The mortality observed following exposure to the 2 formulations of TMOF and TMOF itselfwas less than 20%. Based on the International Organization of Biological Control (IOBC), thetoxicity of TMOF and its formulations against E. kamerunicus can be classified under‘harmless’ (< 30%). This confirms the US EPA report that TMOF and TMOF-based products
  3. 3. show no toxicity or adverse effect against non-target insects, including the oil palmpollinating weevil.ReferenceAbbott, S.W. 1925. A method of computing the effectiveness of an insecticide. J. Econ.Entomol. 18:265-267.Working document on selectivity of pesticides (2005). http://www.iobc-wprs.org/ip_ipm/03021_IOBC_WorkingDocumentPesticides_Explanations.pdf. (Accessed on27 Dec 2010)
  4. 4. Final result for Elaeidobius kamerunicus using recommended rate. 24h 48h 72h TOMF mati hidup mati hidup mati hidup 1 0 25 0 25 2 23 2 0 25 0 25 0 25 3 1 22 2 21 2 21 4 2 20 2 20 2 20 5 0 22 0 22 2 20 control 0 20 0 20 0 20 WP 1 2 20 2 20 3 19 2 0 25 1 24 1 24 3 4 25 4 25 5 24 4 2 24 2 24 3 23 5 0 24 0 24 3 21 control 0 20 0 20 0 20 RH 1 1 25 2 24 2 24 2 2 22 2 22 2 22 3 2 23 5 20 5 20 4 1 25 1 25 2 24 5 1 25 3 23 3 23 control 0 20 0 20 0 20

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