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Els rusislideshow


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Els rusislideshow

  1. 1. Cortisol concentration in the saliva of horses subjected to different kinds of exercise K . Strzelec , M. Kankofer S . Pietrzak’s Els Rusi 2011
  2. 2. Key words <ul><li>Effort </li></ul><ul><li>Equestrian disciplines </li></ul><ul><li>Stress </li></ul><ul><li>Sport horses </li></ul>
  3. 3. Cortisol <ul><li>N atural glucocorticoid hormone produced by the adrenal cortex </li></ul><ul><li>On e of the main substances that indicate stress level </li></ul><ul><li>The most effective way </li></ul><ul><li>of determining cortisol </li></ul><ul><li>concentration in animals </li></ul><ul><li>is using their saliva </li></ul>
  4. 4. Aim <ul><li>Characterize the stress level in sport horses subjected to exercise by measuring the cortisol concentration in their saliva </li></ul>
  5. 5. Methods <ul><li>This study was based on 5 groups of horses doing different types of exercise </li></ul><ul><li>Control group </li></ul><ul><li>Light exercise group </li></ul><ul><li>Three sport groups </li></ul><ul><li>In the research there </li></ul><ul><li>were horses of </li></ul><ul><li>different : </li></ul><ul><li>breed </li></ul><ul><li>age (from 4 to 20 years) </li></ul><ul><li>training program </li></ul>
  6. 6. 3 sport groups <ul><li>1. Show jumping </li></ul><ul><li>2. Dressage </li></ul>
  7. 7. 3. Three-day eventing
  8. 8. Saliva samples <ul><li>Collected 3 × a day: </li></ul><ul><li>Late at night (between 20:00 and 23:00 h) </li></ul><ul><li>Early in the morning (between 5:00 and 8:00 h) </li></ul><ul><li>Directly after the exercise. </li></ul>
  9. 9. Statistical characteristic of salivary cortisol concentration (nmol/dm3) in examined groups of horses <ul><li>a, b, c … Significant differences at p < 0.01 (in columns) </li></ul><ul><li>A, B … Significant differences at p < 0.01 (at lines) </li></ul>0,29 0,71a 1,89 1,41ab 0,41 0,43a 10 S – 3 (dressage) 4,36 8,39cB 1,71 0,81abA 1,58 0,77aA 10 S – 2 (3day event) 5,94 3,98b 2,89 2,43b 4,92 3,45b 10 S – 1 (jumping) 1,39 0,74a 0.05 0,29a 0,58 0,55a 9 Recreational 0 0,28a 0.74 0,49a 0,57 0,45a 10 Control SD Mean SD Mean SD Mean After exercise Early morning Late evening Number of horses Group
  10. 10. Conclusions <ul><li>The highest cortisol concentration was observed for the horses participating in 3-day events (8.93 nmol/dm3), whereas in the control group it was only 0.28 nmol/dm3 </li></ul><ul><li>The stress level as measured by the cortisol concentration in saliva increases with increasing exercise intensity and its duration </li></ul><ul><li>The obtained results do not confirm the existence of a day rhythm of the cortisol concentration in horses </li></ul>
  11. 11. References <ul><li>Acta Veterinaria Brno. 2010 “Cortisol concentration in the saliva of horses subjected to different kinds of exercise” Available at </li></ul>
  12. 12. <ul><li>THANK YOU FOR YOUR ATTENTION! </li></ul>