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Proof load tests can be used for a field assessment of the bridge under study. This paper addresses the determination of the reliability index of an existing bridge by means of proof loading through the case study viaduct De Beek. The information of this bridge is used to determine the updated reliability index after proof load testing. A sensitivity study is carried out to identify the effect of the assumptions with regard to the coefficient of variation on the resistance and load effects. In the current practice of proof load testing with vehicles, it can typically only be demonstrated that a certain vehicle type can cross the bridge safely. The results in this paper provide a new insight on the updating of the reliability index after proof load testing. Consensus on the coefficients of variation that need to be used on the resistance and load effects, is still missing.