Bacillus

3,859 views

Published on

Published in: Technology
0 Comments
6 Likes
Statistics
Notes
  • Be the first to comment

No Downloads
Views
Total views
3,859
On SlideShare
0
From Embeds
0
Number of Embeds
10
Actions
Shares
0
Downloads
148
Comments
0
Likes
6
Embeds 0
No embeds

No notes for slide

Bacillus

  1. 1. Bacillus Aerobic bacilli-B.anthracis Anerobic bacilli-Clostredium tetani
  2. 2. Bacillu anthracis <ul><li>Gram +ve </li></ul><ul><li>Aerobic spore bearing bacilli </li></ul><ul><li>Thermophilic and psuchrophilic </li></ul><ul><li>B.cereus-food borne gastroenteritis </li></ul><ul><li>Produces endospores </li></ul>
  3. 3. <ul><li>First bacterium observed under microscope </li></ul><ul><li>First communicable d/s transmitted by inoculation of infected blood </li></ul><ul><li>First to be isolated In pure culture and used for the preperation of attenuated vaccine </li></ul><ul><li>Used in biological warfere </li></ul><ul><li>One of the largest pathogenic bacteria </li></ul><ul><li>Capsulated PP capsule </li></ul><ul><li>M’Fadyean’s reaction-Polychrome methylene blue for few seconds-purplish material around the bacillus </li></ul><ul><li>Bamboo stick appearences </li></ul><ul><li>Nonmotile </li></ul><ul><li>Spores – central /terminal </li></ul>
  4. 4. Culture <ul><li>Agar plates –frost glass appearance </li></ul><ul><li>Low power –medusa plate appearance </li></ul><ul><li>Gelatin stab culture-inverted fir tree </li></ul><ul><li>Blood agar-nonhemolytic </li></ul><ul><li>Selective medium-PLET medium- </li></ul>
  5. 5. Virulence factors <ul><li>Capsular PP </li></ul><ul><li>Anthra toxin </li></ul><ul><li>Pathogenicity testing by inoculation into guinea pigs </li></ul>
  6. 6. Anthrax <ul><li>Zoonosis </li></ul><ul><li>Human d/s from animal-direct /indirect </li></ul><ul><li>d/s may be </li></ul><ul><li>Cutaneous </li></ul><ul><li>Pulmonary </li></ul><ul><li>Intestinal </li></ul><ul><li>All leading to fatal septicemia/meningitis </li></ul>
  7. 7. Cutaneous <ul><li>Entry thru skin </li></ul><ul><li>Satellite lasions arranged around a central necrotic lesion which is coverd by a black eschar- the name anthrax meaning coal comes from the black colour of eschar </li></ul><ul><li>These lesions are called Malignant pustules </li></ul><ul><li>Hide porter’s d/s-common in dock workers-carrying loads and skins on their bare back. </li></ul><ul><li>Generally resolves spaontaneously </li></ul>
  8. 8. Pulmonary <ul><li>Wool sorte’s d/s </li></ul><ul><li>Inhalation of infected wool particles </li></ul><ul><li>Haemorrhagic pneumonia with high fatality rates </li></ul><ul><li>Haemorrhagic meningitis is a main complication </li></ul>
  9. 9. Intestinal <ul><li>Rare </li></ul><ul><li>Violent enteritis </li></ul><ul><li>Bloody diarrhoea </li></ul><ul><li>Primitive communities who eat carcasses of animals </li></ul>
  10. 10. Lab d/g <ul><li>Microscopy, culture,M’fadyeans reaction </li></ul><ul><li>Serology-CFT,ELISA </li></ul><ul><li>Ascol’s thermo precipitation test-demonstration of anthrax antigen in tissue extracts </li></ul><ul><li>Nucleic acid detection </li></ul>
  11. 11. Treatment <ul><li>DOC-Penicillin </li></ul><ul><li>Streptomycin </li></ul><ul><li>Doxycycline </li></ul>
  12. 12. Prophylaxis <ul><li>Improvement of factory hygiene </li></ul><ul><li>Proper sterilization </li></ul><ul><li>Proper disposal of animal carcasses </li></ul><ul><li>Vaccination of animals </li></ul>

×