Extracellular recording system to trap adenosine triphosphate (ATP) released from a taste bud cell of α-gustducin–green fluorescence protein (Gust-GFP) mice. A: merged confocal and differential interference contrast images around the stimulating pipette tip with a fungiform taste bud in the experimental setup. B: sample records from a GFP-positive taste bud cell. NaCl, 300 mM; saccharin (Sac), 20 mM; HCl, 10 mM; cyclohexamide (Chx), 500 μM; monosodium glutamate (MSG), 300 mM. C: representative ATP release from a GFP-positive (a) and -negative (b) taste bud cell by application of 20 mM Sac. Left: extracellular recording of electrical activity (arrowheads indicate offset of bath perfusion and onset of taste stimulation). Right: ATP concentration in the recording electrode solution measured by luciferase assay (N.D., not detectable; detection limit, 40 pM).
CALCIUM-SENSING RECEPTOR (CaSR) - НОВИЙ
КАНДИДАТ НА РОЛЬ ШЛУНКОВОГО
C: overlay of stainings against CaSR (red)
and the H+
-ATPase -subunit (green)β
demonstrates expression of both proteins
in the same cells.
The calcium-sensing receptor acts as a
modulator of gastric acid secretion in
freshly isolated human gastric glands
Matthias M. Dufner, Philipp Kirchhoff
CFTR - ion channel that transports
chloride and thiocyanate ions (SCN–
across epithelial cell membranes.
найбільш розповсюджене аутосомно-рецесивне захворювання
людини (1 випадок на 2000-2500 новонароджених), що виникає
внаслідок мутацій в гені CFTR (Cystic fibrosis transmembrane