Successfully reported this slideshow.
We use your LinkedIn profile and activity data to personalize ads and to show you more relevant ads. You can change your ad preferences anytime.

Pathology ospe revised edition

8,524 views

Published on

Pathology ospe revised edition

Published in: Education, Technology, Business
  • excellent presentation, enriched the knowledge to for students
       Reply 
    Are you sure you want to  Yes  No
    Your message goes here
  • do the slides move ? i mean its just one slide here (the main one) . where are the others
       Reply 
    Are you sure you want to  Yes  No
    Your message goes here

Pathology ospe revised edition

  1. 1. abbas_naseem@yahoo.com Dated: Sep 30, 2010
  2. 2. Ascaris Lumbricoides(Round worms) – microscopically unsegmented ovum By: Abbas Naseem (BDS) abbas_naseem@yahoo.com 2
  3. 3. Transistional Cell CarcinomafibroVascularcore Papillae structure Anilline dye -- bladder tumor By:Abbas Naseem (BDS) abbas_naseem@yahoo.com 3
  4. 4. Transistional Epithelia Cell core12 layers (minimum 7 layers) By: Abbas Naseem (BDS) abbas_naseem@yahoo.com 4
  5. 5. Chocolate agar Chocolate agar (CHOC) - is a non-selective, enriched growth medium. It is a variant of the blood agar plate. It contains red blood cells, which have been lysed by heating very slowly to 56 °C. Chocolate agar is used for growing fastidious (fussy) respiratory bacteria, such as Haemophilus influenzae. These bacteria need growth factors, like NAD and hematin, which are inside erythrocytes; thus, a prerequisite to growth is lysis of the red blood cells By: Abbas Naseem (BDS) abbas_naseem@yahoo.com 5
  6. 6. Blood Agar A nutrient culture medium that is enriched with whole blood and used for the growth of certain strains of bacteria. Contains mammalian blood (usually sheep or horse), typically at a concentration of 5–10%. BAP are an enriched, differential media used to isolate fastidious organisms and detect hemolytic activity. β-hemolytic activity will show complete lysis of red blood cells surrounding colony, while α-hemolysis will only partially lyse hemoglobin and will appear green. γ-hemolysis (or non-hemolytic) is the term referring to a lack of hemolytic activity. By: Abbas Naseem (BDS) abbas_naseem@yahoo.com 6
  7. 7. micropipette used to measure very small volumes of liquids. By: Abbas Naseem (BDS) abbas_naseem@yahoo.com 7
  8. 8.  Elisa microtitre plate OR Elisa Kit Serological measurements, like measurment of Hepatitus B Elisa +ive PCR Quanitative Qualitative By: Abbas Naseem (BDS) abbas_naseem@yahoo.com 8
  9. 9. Dystrophic Calcification ----- Dark basophilicQ.Give causes of dystrophic calcificationQ.Give 3 examples of dystrophic calcificationQ.Psamoma Bodies ? By: Abbas Naseem (BDS) abbas_naseem@yahoo.com 9
  10. 10. Liquifactive Necrosis of BrainCommonest cause: Bacterial infections By: Abbas Naseem (BDS) abbas_naseem@yahoo.com 10
  11. 11.  Salmonella shigella agar plate with H2S producing colonies Black color colonies By: Abbas Naseem (BDS) abbas_naseem@yahoo.com 11
  12. 12. Coagulative NecrosisName two other organs? By: Abbas Naseem (BDS) abbas_naseem@yahoo.com 12
  13. 13. Ventricular hypertrophy of heartInfarction/Necrosis By: Abbas Naseem (BDS) abbas_naseem@yahoo.com 13
  14. 14. Entamoeba histolytica By: Abbas Naseem (BDS) abbas_naseem@yahoo.com 14
  15. 15. Entamoeba histolytica By: Abbas Naseem (BDS) abbas_naseem@yahoo.com 15
  16. 16. By: Abbas Naseem (BDS)abbas_naseem@yahoo.com 16
  17. 17. Well differentiation Invasive Sq.Cell CarcinomaKeratinization pearlsIntracellular bridge By: Abbas Naseem (BDS) abbas_naseem@yahoo.com 17
  18. 18. billirubin By: Abbas Naseem (BDS) abbas_naseem@yahoo.com 18
  19. 19.  The Lowenstein-Jensen medium, popularly known as LJ medium, is agrowth medium specially usedfor culture of Mycobacterium,notably Mycobacterium tuberculosis. When grown on LJ medium, M. tuberculosis appears as brown, granule-like colonies. The media must be incubated for a significant length of time, usually four weeks, due to the slow doubling time of M. tuberculosis compared with other bacteria (15-20 hours). By: Abbas Naseem (BDS) abbas_naseem@yahoo.com 19
  20. 20. ZN StainGold standart of T.B….to confirm it Z.N stain By: Abbas Naseem (BDS) abbas_naseem@yahoo.com 20
  21. 21. Endometrial hyperplasiaWhy its imp?Bc. Leads to adenocarcinoma By: Abbas Naseem (BDS) abbas_naseem@yahoo.com 21
  22. 22. Why called? Fibrinoids Bc of fibre like apperance BENIGN Submucosal IntraMural SubserosalEnlist 4 other mucosal tumors?What is the malignant counterpart of this tumor? LEIMYOSARCOMA By: Abbas Naseem (BDS) abbas_naseem@yahoo.com 22
  23. 23. MalignantIll defined mass with necrosis & haemorrhageQ.Enlist any 4 other malignant tumors? By: Abbas Naseem (BDS) abbas_naseem@yahoo.com 23
  24. 24. Malignant tumor Biazarre cells, mitosis Q.Enlist any four features of malignant cells/ By: Abbas Naseem (BDS) abbas_naseem@yahoo.com 24
  25. 25. DysplasiaQ. How will you grade dysplasia?Mild Q. What is carinoma in Situ?ModeratorSevere By: Abbas Naseem (BDS) abbas_naseem@yahoo.com 25
  26. 26. Acute inflammation of Appendicitis neutrophils By: Abbas Naseem (BDS) abbas_naseem@yahoo.com 26
  27. 27. Acute Appendicitis By: Abbas Naseem (BDS) abbas_naseem@yahoo.com 27
  28. 28. By: Abbas Naseem (BDS)abbas_naseem@yahoo.com 28
  29. 29. AppendicitisQ. Why tip of appendicitis is important?Bc appendicitis tumor arise from there..Q. Commnest cause of appendicitis: Carcinoid Abbas Naseem (BDS) By: Tumor (neuroendocrine tumor abbas_naseem@yahoo.com 29
  30. 30. Lipoma By: Abbas Naseem (BDS) abbas_naseem@yahoo.com 30
  31. 31. D latum – fish tapeworm – oval – operculum at one end ! By: Abbas Naseem (BDS) abbas_naseem@yahoo.com 31
  32. 32. Widal test interpretations Dilutions are made :Serum: Water 1:20 1:40 1:80 1:160 --- positive ( +++) 1: 320 +++To check Active phase of typhoid.Q.Name two other test for typhoid?Typhi dot test By: Abbas Naseem (BDS) abbas_naseem@yahoo.com 32
  33. 33. Goblet Cells Barret Esophagus – intestinal METAPLASIA Squamous  Columnar By: Abbas Naseem (BDS) abbas_naseem@yahoo.com 33
  34. 34. Fat Necrosis / Dystrophic CalcificationQ. What are the blood calcium levels?normal By: Abbas Naseem (BDS) abbas_naseem@yahoo.com 34
  35. 35. InfarctionQ.Def?Q. Red/white Infarction? By: Abbas Naseem (BDS) abbas_naseem@yahoo.com 35
  36. 36. Exudate, Plasma Cells…Chronic Inflammatory Cells By: Abbas Naseem (BDS) abbas_naseem@yahoo.com 36
  37. 37. Fibroblasts, newly formed blood vessels, macrophages ( GRANULATION TI Q.Difference btw GRANULOMA & GRANULATION TISSUE? By: Abbas Naseem (BDS) abbas_naseem@yahoo.com 37
  38. 38.  Cysteine Lactose Electrolyte Deficient agar (CLED) ◦ CLED agar is used to isolate and differentiate urinary tract bacteria, since it inhibits Proteus species swarming and can differentiate between lactose fermenters and non-fermenters. By: Abbas Naseem (BDS) abbas_naseem@yahoo.com 38
  39. 39. Fatty ChangeCommon Site: LiverCommon cause: Alcohol By: Abbas Naseem (BDS) abbas_naseem@yahoo.com 39
  40. 40. Folded epithelium lined by benign columnar epithelial cells By: Abbas Naseem (BDS) abbas_naseem@yahoo.com 40
  41. 41. IRONPearls prussan blue stain for iron By: Abbas Naseem (BDS) abbas_naseem@yahoo.com 41
  42. 42. Classical malarial parasite; ring form By: Abbas Naseem (BDS) abbas_naseem@yahoo.com 42
  43. 43. Entrobius vermicularis – typical D shaped ! ( pinworm ) By: Abbas Naseem (BDS) abbas_naseem@yahoo.com 43
  44. 44. Ascaris lumbricoides ( giant round worm – brown with mamellations n wavy margins ) By: Abbas Naseem (BDS) abbas_naseem@yahoo.com 44
  45. 45. Granuloma (OLD)b.c of fibrosis By: Abbas Naseem (BDS) abbas_naseem@yahoo.com 45
  46. 46. By: Abbas Naseem (BDS)abbas_naseem@yahoo.com 46
  47. 47. Edge of nest * peripheral palisadingTumor arise from skinBasal cell Carcinoma + CNS doesn’t spread (just locally spread) By: Abbas Naseem (BDS) abbas_naseem@yahoo.com 47
  48. 48. GranulomaLanghan giant cellsClassical epithelioid cells By: Abbas Naseem (BDS) abbas_naseem@yahoo.com 48
  49. 49. Hemosiderin in Macrophages Hemosiderosis VS. Hemochromatosis Reversible Irreversible No Iron absorption Excess Iron Organ becomes dysfunctional By: Abbas Naseem (BDS) abbas_naseem@yahoo.com 49
  50. 50. Anthracotic CarbonAnthracosis: Accumulation of Carbon in macrophages By: Abbas Naseem (BDS) abbas_naseem@yahoo.com 50
  51. 51. Gall Stones Carcinoma of gall bladder Commonest carcinoma of gall bladder: adenocarcinoma By: Abbas Naseem (BDS)TIP: epithelial columnar; Tumor AdenoCarcinoma abbas_naseem@yahoo.com 51
  52. 52. Caseous Necrosis By: Abbas Naseem (BDS) abbas_naseem@yahoo.com 52
  53. 53. MacConkey agar (MAC) A selective and differential media used to differentiate between Gram negative bacteria while inhibiting the growth of Gram positive bacteria. The addition of bile salts and crystal violet to the agar inhibits the growth of most Gram positive bacteria, making MacConkey agar selective. Lactose and neutral red are added to differentiate the lactose fermenters, which form pink colonies, from lactose nonfermenters that form clear colonies. By: Abbas Naseem (BDS) abbas_naseem@yahoo.com 53
  54. 54. Atrophy Mechanism; Ubiquitin-Proteasome pathway By: Abbas Naseem (BDS) abbas_naseem@yahoo.com 54
  55. 55. Lipoma; No backgroundQ. What is the counterpart of this: LIPOSARCOMA By: Abbas Naseem (BDS) abbas_naseem@yahoo.com 55
  56. 56. Hook worm ( ankylostoma duodenale ) – transparent egg shell– oval – blastomere ! By: Abbas Naseem (BDS) abbas_naseem@yahoo.com 56
  57. 57. AdenoCarcinoma …Wall-Sharing By: Abbas Naseem (BDS) abbas_naseem@yahoo.com 57
  58. 58. LeomyomaNo necrosisNo mitosis By: Abbas Naseem (BDS) abbas_naseem@yahoo.com 58
  59. 59. Trichuris trichura – whipworm – barrel shaped – brown – mucusplugs at ends – segmented ovum ! By: Abbas Naseem (BDS) abbas_naseem@yahoo.com 59
  60. 60. Lines of Zahn By: Abbas Naseem (BDS) abbas_naseem@yahoo.com 60
  61. 61. Benign Lipoma By: Abbas Naseem (BDS) abbas_naseem@yahoo.com 61
  62. 62. LipoSarcoma By: Abbas Naseem (BDS) abbas_naseem@yahoo.com 62
  63. 63. By: Abbas Naseem (BDS)abbas_naseem@yahoo.com 63
  64. 64. Sensitivity plate By: Abbas Naseem (BDS) abbas_naseem@yahoo.com 64
  65. 65. Councilmen Bodies: Spcial apoptotic bodies Liver hepatitusFoldings of sq.epiSquamous Papilloma (benign)Common site: skin, oral By: Abbas Naseem (BDS) abbas_naseem@yahoo.com 65
  66. 66. Renal InfarctionWedge Shaped By: Abbas Naseem (BDS) abbas_naseem@yahoo.com 66
  67. 67. GangreneWhat is Gangrenous Necrosis?Diabetesimmunocompromised By: Abbas Naseem (BDS) abbas_naseem@yahoo.com 67
  68. 68.  Sabouraud agar is used to culture fungi and has a low pH that inhibits the growth of most bacteria; also contains the antibiotic gentamicin to specifically inhibit the growth of Gram-negative bacteria. By: Abbas Naseem (BDS) abbas_naseem@yahoo.com 68
  69. 69. Triple Sugar Iron STAIN By: Abbas Naseem (BDS) abbas_naseem@yahoo.com 69
  70. 70. Triple Sugar Iron medium Triple Sugar Iron medium is a differential medium that can distinguish between a number of Gram-negative enteric bacteria based on their physiological ability (or lack thereof) to: a. metabolize lactose and/or sucrose b. conduct fermentation to produce acid c. produce gas during fermentation d. generate H2S. By: Abbas Naseem (BDS) abbas_naseem@yahoo.com 70
  71. 71. Triple Sugar Iron medium The medium contains 1.0% each of sucrose and lactose and 0.1% glucose. If only glucose is fermented, acid produced in the butt will turn it yellow, but insufficient acid products are formed to affect the methyl red in the slant. However, if either sucrose or lactose are fermented, sufficient fermentation products will be formed to turn both the butt and the slant yellow. If gas is formed during the fermentation, it will show in the butt either as bubbles or as cracking of the agar. If no fermentation occurs (as for an obligate aerobe), the slant and butt will remain red. By: Abbas Naseem (BDS) abbas_naseem@yahoo.com 71
  72. 72. ANAEROBIC JAR By: Abbas Naseem (BDS) abbas_naseem@yahoo.com 72
  73. 73. G. +ive Cocci By: Abbas Naseem (BDS) abbas_naseem@yahoo.com 73
  74. 74. Atrophy Brain By: Abbas Naseem (BDS) abbas_naseem@yahoo.com 74
  75. 75. Caseous Necrosis By: Abbas Naseem (BDS) abbas_naseem@yahoo.com 75
  76. 76. BPH- Benign ProstaticHyperplasia By: Abbas Naseem (BDS) abbas_naseem@yahoo.com 76
  77. 77. LIKE MYPRESENTATION?For Comments &Suggestions:abbas_naseem@yahoo.com Abbas Naseem Dated: Sep 30, 2010

×