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management of diagnostic microbiology laboratory

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Management of diagnostic microbiology laboratory by Dr.T.V.Rao MD

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management of diagnostic microbiology laboratory

  1. 1. OF DIAGNOSTIC MICROBIOLOGY LABORATORY Dr.T.V.Rao MD 4/7/2016Dr.T.V.Rao MD 1
  2. 2. ROLE OF MICROBIOLOGY IN HOSPITAL • The critical role of the microbiology laboratory in infectious disease diagnosis calls for a close, positive working relationship between the physician and the microbiologists who provide enormous value to the health care team 4/7/2016Dr.T.V.Rao MD 2
  3. 3. BIOSAFETY IS CONCERN FOR ALL WHO WORK WITH HAZARDOUS PATHOGENS AND MATERIALS • The backbone of the practice of biosafety is risk assessment. While there are many tools available to assist in the assessment of risk for a given procedure or experiment, the most important component is professional judgement • Risk assessments should be performed by the individuals most familiar with the specific characteristics of the organisms being considered for use, the equipment and procedures to be employed, animal models that may be used, and the containment equipment and facilities available. 4/7/2016Dr.T.V.Rao MD 3
  4. 4. WHO MANAGES BIOSAFETY • It is the responsibility of the laboratory director (the person who has immediate responsibility for the laboratory) to ensure the development and adoption of a biosafety management plan and a safety or operations manual. 4/7/2016Dr.T.V.Rao MD 4
  5. 5. TRAIN THE PEOPLE WITH SCIENTIFIC KNOWLEDGE • One of the most helpful tools available for performing a microbiological risk assessment is the listing of risk groups for microbiological agents 4/7/2016Dr.T.V.Rao MD 5
  6. 6. FACTORS THAT SHOULD BE CONSIDERED, AS APPROPRIATE, INCLUDE • Pathogenicity of the agent and infectious dose 2. Potential outcome of exposure 3. Natural route of infection 4. Other routes of infection, resulting from laboratory manipulations (parenteral, airborne, ingestion) 5. Stability of the agent in the environment 4/7/2016Dr.T.V.Rao MD 6
  7. 7. FACTORS THAT SHOULD BE CONSIDERED, AS APPROPRIATE, INCLUDE • 6 Concentration of the agent and volume of concentrated material to be manipulated 7. Presence of a suitable host (human or animal) 8. Information available from animal studies and reports of laboratory- acquired infections or clinical reports 9. Laboratory activity planned (sonication, aerosolization, centrifugation, etc.) 10.Any genetic manipulation of the organism that may extend the host range of the agent or alter the agent’s sensitivity to known, effective treatment regimens 11.Local availability of effective prophylaxis or therapeutic interventions. 4/7/2016Dr.T.V.Rao MD 7
  8. 8. SPECIMENS FOR WHICH THERE IS LIMITED INFORMATION IT IS PRUDENT TO TAKE A CAUTIOUS APPROACH TO SPECIMEN MANIPULATION. • 1. Standard precautions should always be followed, and barrier protections applied (gloves, gowns, eye protection), whenever samples are obtained from patients. 2. Basic containment – Biosafety Level 2 practices and procedures should be the minimum requirement for handling specimens. 3. Transport of specimens should follow national and/or international rules and regulations. 4/7/2016Dr.T.V.Rao MD 8
  9. 9. DETERMINING THE RISK OF HANDLING THESE SPECIMENS: • 1. Medical data on the patient 2. Epidemiological data (morbidity and mortality data, suspected route of transmission, other outbreak investigation data) 3. Information on the geographical origin of the specimen 4/7/2016Dr.T.V.Rao MD 9
  10. 10. NEEDS CONTINUOUS TRAINING AND MONITORING THE STAFF • The laboratory supervisor (reporting to the laboratory director) should ensure that regular training in laboratory safety is provided. 4/7/2016Dr.T.V.Rao MD 10
  11. 11. STAFF SHOULD BE CAUTIONED ON SAFETY ISSUES • Personnel should be advised of special hazards, and required to read the safety or operations manual and follow standard practices and procedures. The laboratory supervisor should make sure that all personnel understand these. A copy of the safety or operations manual should be available in the laboratory 4/7/2016Dr.T.V.Rao MD 11
  12. 12. SAFETY OF THE STAFF A PRIORITY • Appropriate medical evaluation, surveillance and treatment should be provided for all personnel in case of need, and adequate medical records should be maintained 4/7/2016Dr.T.V.Rao MD 12
  13. 13. DESIGN FEATURES 4/7/2016Dr.T.V.Rao MD 13
  14. 14. LABORATORY DESIGN AND FACILITIES , SPECIAL ATTENTION SHOULD BE PAID TO CONDITIONS THAT ARE KNOWN TO POSE SAFETY PROBLEMS • 1 Formation of aerosols 2. Work with large volumes and/or high concentrations of microorganisms 3. Overcrowding and too much equipment 4. Infestation with rodents and arthropods 5. Unauthorized entrance 6. Workflow: use of specific samples and reagents 4/7/2016Dr.T.V.Rao MD 14
  15. 15. 4/7/2016Dr.T.V.Rao MD 15
  16. 16. LABORATORIES SHOULD HAVE FACULTIES FOR 4/7/2016Dr.T.V.Rao MD 16
  17. 17. WORKING WITH UNGLOVED HAND PUTS US AT RISK IN LABORATORY GLOVES INCREASE BIOSAFETY 4/7/2016Dr.T.V.Rao MD 17
  18. 18. BUILDING THE DIAGNOSTIC MICROBIOLOGY DEPARTMENTS • Ample space must be provided for the safe conduct of laboratory work and for cleaning and maintenance. 2. Walls, ceilings and floors should be smooth, easy to clean, impermeable to liquids and resistant to the chemicals and disinfectants normally used in the laboratory. Floors should be slip- resistant. 4/7/2016Dr.T.V.Rao MD 18
  19. 19. BENCH TOPS SHOULD BE CLEAN AND SAFE • 3 Bench tops should be impervious to water and resistant to disinfectants, acids, alkalis, organic solvents and moderate heat. 4. Illumination should be adequate for all activities. Undesirable reflections and glare should be avoided 4/7/2016Dr.T.V.Rao MD 19
  20. 20. LABORATORY FURNITURE SHOULD BE CLEANS AND STURDY • Laboratory furniture should be sturdy. Open spaces between and under benches, cabinets and equipment should be accessible for cleaning 4/7/2016Dr.T.V.Rao MD 20
  21. 21. LABORATORY STORAGE • Storage space must be adequate to hold supplies for immediate use and thus prevent clutter on bench tops and in aisles. Additional long-term storage space, conveniently located outside the laboratory working areas, should also be provided. 4/7/2016Dr.T.V.Rao MD 21
  22. 22. NO EATING AND DRINKING IN LABORATORY TO BE PERMITTED • Facilities for eating and drinking and for rest should be provided outside the laboratory working areas 4/7/2016Dr.T.V.Rao MD 22
  23. 23. HAND WASHING BASINS IN LABORATORY A MUST • Hand-washing basins, with running water if possible, should be provided in each laboratory room, preferably near the exit door 4/7/2016Dr.T.V.Rao MD 23
  24. 24. BIO SAFETY IN LABORATORY • At Biosafety Level 2, an autoclave or other means of decontamination should be available in appropriate proximity to the laboratory. .Safety systems should cover fire, electrical emergencies, emergency shower and eyewash facilities. 4/7/2016Dr.T.V.Rao MD 24
  25. 25. DO NOT USE WET MOPS IN THE LABORATORY USE DRY MOPS 4/7/2016Dr.T.V.Rao MD 25
  26. 26. HAND WASHING FACILITIES REDUCES THE SPREAD OF INFECTIONS • .A dependable supply of good quality water is essential. There should be no cross connections between sources of laboratory and drinking-water supplies. An ant backflow device should be fitted to protect the public water system. 4/7/2016Dr.T.V.Rao MD 26
  27. 27. • Program created by Dr.T.V.Rao MD for Medical and paramedical Professionals who wish to run and establish the Diagnostic Microbiology with principles of safety • Email • doctortvrao@gmail.com 4/7/2016Dr.T.V.Rao MD 27

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