Preparation and characterization cellulose draft final final

744 views

Published on

Published in: Technology, Business, Lifestyle
0 Comments
0 Likes
Statistics
Notes
  • Be the first to comment

  • Be the first to like this

No Downloads
Views
Total views
744
On SlideShare
0
From Embeds
0
Number of Embeds
22
Actions
Shares
0
Downloads
23
Comments
0
Likes
0
Embeds 0
No embeds

No notes for slide
  • ANADIR FOTOOOOOS!!
  • Porosidad gravimetrica
  • Preparation and characterization cellulose draft final final

    1. 1. Preparation and Characterization of Cellulose Fibers for Biochemical Applications Delaine Zayaz-Bazán 1 , José A. Cruz-Arzón 1 , Nelson Granda-Paz 1 , and Wilfredo OtañoRivera 3,4 1-Department of Biology, University of Puerto Rico at Cayey, Puerto Rico 1-Department of Chemistry, University of Puerto Rico at Mayagüez, Puerto Rico 3- Department of Physics-Mathematics, University of Puerto Rico at Cayey, Puerto Rico 4-Institute for Functional Nanomaterials, University of Puerto Rico, San Juan, Puerto Rico
    2. 2. Problem • Is it possible to prepare electrospun cellulose fibers that could be used for separation of proteins producing high flow and mass transfer?
    3. 3. Objectives • Prepare cellulose acetate fibers by electrospinning and transform them into cellulose by a deacetylation process • Characterize fibers by SEM and FTIR among other techniques. • Evaluate chemical and physical electrospinning process parameters in order to craft porosity of cellulose fibers.
    4. 4. Basic Concepts Cellulose Acetate Cellulose • Molar Mass 30,000 • Molar Mass  600,000 • Density 1.3 g/cm3 • Density  1.5 g/cm3
    5. 5. • Cellulose Acetate for fiber synthesis • Cellulose for protein separation
    6. 6. Methodology
    7. 7. Methodology + Cellulose acetate Acetone/DMF/EtOH Cellulose 21% sol
    8. 8. Electrospinning • Typical conditions ▫ Distance: 18 cm ▫ 20.0 kV ▫ 0.5 mL/ hr
    9. 9. Results
    10. 10. Results 21% CA fibers
    11. 11. Fiber Diameter Average 693 ±306
    12. 12. Deacetylation OHRegenerated Cellulose Membrane
    13. 13. FTIR Analysis 1 AC.jws.dx 0.9 0.8 0.7 0.6 0.5 0.4 Transmittance 0.3 0.2 0.1 0 5000 1745 4500 4000 3500 3000 2500 Wavenumbers [1/cm] 2000 1500 1000 500
    14. 14. FTIR Analysis 1740
    15. 15. Deacetylation • Cellulose Acetate Esther Functional Group • Cellulose Hydroxyl Group
    16. 16. FTIR Analysis 0.14 control #2 C Vibha Smooth.jws.dx smoothed smoothed m#D CR.jws.dx smoothed smoothed 0.12 0.1 0.08 0.06 Transmittance 0.04 0.02 1640 0 -0.02 5000 4500 4000 3500 3000 2500 W avenumbers [1/cm] 2000 1500 1000 500 0
    17. 17. Regenerated vs. Comercial Cellulose
    18. 18. Porosity Measurements ±9
    19. 19. Conclusions • The work is still going on ▫ The preliminary results show that cellulose can be regenerated from the acetate • Future Works
    20. 20. Acknowledgements • UPR at Cayey • RISE Program • NAMAS Lab • Our Lab Group • Professor Vibha Bansal
    21. 21. Thank You Questions?

    ×