Streptococus deepa


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Streptococus deepa

  1. 1. STREPTOCOCCUS B,C,D,VIRIDANS DEEPA BABIN ASST PROF Travancore Medical College, Kollam
  2. 2. Hemolyic patterns on blood agar
  3. 3. Group B: Streptococcus agalactiae• Regularly resides in human vagina, pharynx, and large intestine• Can be transferred to infant during delivery and cause severe infection – Pregnant women should be screened and treated• Wound and skin infections and endocarditis in debilitated people
  4. 4. Deepa Babin 5 LAB DIAGNOSIS Cultivation and diagnosis ensure proper treatment to prevent possible complications Rapid diagnostic tests based on monoclonal antibodies that react with C-carbohydrates Culture using bacitracin disc test, CAMP test, Hippurate hydrolysis
  5. 5. Deepa Babin 6 CAMP Test- S.agalactiae• By Christie, Atkins, and Munch-Peterson• In the CAMP test, group B streptococci release a soluble factor (CAMP factor) that acts synergistically with the -hemolysin of Staphylococcus aureus.• A strain of -hemolysin producing Staphylococcus aureus is inoculated down the center of a sheep blood agar plate.• A single streak of an isolate to be identified is inoculated perpendicular to the S. aureus streak to within 3-4 mm of the S. aureus streak.• The blood agar plate is then incubated overnight in an 35-37oC air incubator• If a large arrowhead zone of enhanced -hemolysis develops between the unknown isolate and S. aureus the unknown isolate is group B Streptococcus.
  6. 6. • Groups C –Strep equisimilis species – Common animal flora, frequently isolated from upper respiratory; pharyngitis,osteomylitis,glomerulonephritis, bactere mia,brain abscess,puerperal sepsis – Strep equisimilis- source of streptokinase Group G,H,K O,R: Group G- commensals of throat of man monkey dogs Occationaly-tonsilitis,endocarditis,UTI Group H and K- Endocarditis Group O- tonsilitis,endocarditis Group R- Pigs(Septicemia,meningitis)
  7. 7. Treatment• Groups A and B are treated with Penicillin• Long-term penicillin prophylaxis for people with a history of rheumatic fever or recurrent strep throat
  8. 8. Deepa Babin 10 Group D (Enterococci)• Two groups- Enterococcal gp and Non Enterococcal gp – Enterococcus faecalis, E. faecium, E. durans – Strep bovis,Strep equinus- Non Enterococcal gp
  9. 9. Deepa Babin 11 Group D (Enterococci)• -Normal colonists of human large intestine,Genital tract and saliva – Cause opportunistic urinary, wound, and skin infections, particularly in debilitated persons – Intra abdominal abscess complicating diverticulitis and peritonitis
  10. 10. Lab diagnosis• Bile Esculin hydrolysis – Ability to grow in 40% bile and hydrolyze Esculin are features of Enterococci that possess Group D antigen• Growth in 6.5% NaCl broth – Differentiates Group D streptococci from enterococci
  11. 11. Treatment• Treatment-Penicillin- Resistance(enterococci)• P –R ,CHOICE IS VANCOMYCIN• VRE- Vancomycin resitant Enterococci• Enterococcal treatment usually requires combined therap• Cephalosporins are inherently resistant for Enterococci
  12. 12. Deepa Babin 14 Viridans Group• Bacteremia, meningitis, abdominal infection, tooth abscesses• Most serious infection – Sub Acute Endocarditis – Blood- borne bacteria settle and grow on heart lining or valves• S. mutans produce slime layers that adhere to teeth, basis for plaque• Involved in dental caries• Persons with pre existing heart conditions should receive prophylactic antibiotics before surgery or dental procedures• Persons with pre existing heart disease are at high risk• Colonization of heart by forming biofilms
  13. 13. Deepa Babin 15