seminar ppt

21,524 views

Published on

This is a upload-testing document.

Published in: Education, Technology, Business
  • Be the first to like this

seminar ppt

  1. 1. Identification of Genes required for Cytoplasmic Localization in Early C. elegans Embryos
  2. 2. Background
  3. 3. Development of C.elegans
  4. 4. Development <ul><li>After fertilization, the two pronucleis join together. </li></ul><ul><li>http://www.mbg.cornell.edu/cals/mbg/research/kemphues-lab/movies.cfm/kjk1_wt </li></ul>
  5. 5. Development <ul><li>Then the mitotic spindle forms, and it migrates posteriorly. </li></ul><ul><li>http://www.mbg.cornell.edu/cals/mbg/research/kemphues-lab/movies.cfm/kjk1_wt </li></ul>
  6. 6. Development <ul><li>Owing to the migration, the two daughter blastomeres are produced in different sizes after the first cleavage. </li></ul><ul><li>http://www.mbg.cornell.edu/cals/mbg/research/kemphues-lab/movies.cfm/kjk1_wt </li></ul>
  7. 7. Development <ul><li>At the second cleavage, AB divides transversely and P 1 divides longitudinally. AB always divides before P 1 . </li></ul><ul><li>http://www.mbg.cornell.edu/cals/mbg/research/kemphues-lab/movies.cfm/kjk1_wt </li></ul>
  8. 8. Principles of Development (Lewis Wholper) Chapter 5
  9. 9. <ul><li>After fertilization, the two pronucleis join together. </li></ul><ul><li>Mitotic spindle forms, and it migrates posteriorly. </li></ul><ul><li>Daughter blastomeres are produced in different sizes after the first cleavage. </li></ul><ul><li>AB > P1 </li></ul><ul><li>P granules are localized to P1 </li></ul><ul><li>At the second cleavage, AB divides transversely and P1 divides longitudinally. </li></ul><ul><li>AB always divides before P1 </li></ul><ul><li>P granules are localized to P2 </li></ul>
  10. 10. <ul><li>After fertilization, the two pronucleis join together. </li></ul><ul><li>Mitotic spindle forms, and it migrates posteriorly. </li></ul><ul><li>Daughter blastomeres are produced in different sizes after the first cleavage. </li></ul><ul><li>AB > P1 </li></ul><ul><li>P granules are localized to P1 </li></ul><ul><li>At the second cleavage, AB divides transversely and P1 divides longitudinally . </li></ul><ul><li>AB always divides before P1 </li></ul><ul><li>P granules are localized to P2 </li></ul>
  11. 11. The Result
  12. 12. Genes
  13. 13. Defects <ul><li>Equal First Cleavage </li></ul><ul><li>Altered Orientation of Second Cleavages </li></ul><ul><li>Synchronous Cleavages </li></ul><ul><li>Abnormal Localization of P Granule </li></ul>
  14. 14. Aim <ul><li>To identify the genes required for cytoplasmic localization </li></ul><ul><li>in early C. elegans embryos </li></ul>
  15. 15. Sub-Questions
  16. 16. <ul><li>http://www.mbg.cornell.edu/cals/mbg/research/kemphues-lab/research-details.cfm </li></ul>
  17. 17. Identify The Genes <ul><li>Screening </li></ul><ul><li>There are four categories of mutation, but three of them </li></ul><ul><li>are hard to observe. </li></ul><ul><li>So they chose the forth. </li></ul><ul><li>Linkage Mapping </li></ul><ul><li>Linkage Analysis </li></ul><ul><li>Meiotic Recombination </li></ul><ul><li>Complementation </li></ul>
  18. 18. Identify The Defects <ul><li>Equal First Cleavage </li></ul><ul><li>Altered Orientation of Second Cleavages </li></ul><ul><li>Synchronous Cleavages </li></ul><ul><li>Abnormal Localization of P Granule </li></ul>
  19. 19. Equal First Cleavage
  20. 20. Equal First Cleavage <ul><li>Blastomere Size Measurement </li></ul><ul><li>Spindle Movement Measurement </li></ul>
  21. 21. Equal First Cleavage
  22. 22. Equal First Cleavage
  23. 25. Altered Second Cleavages
  24. 26. Altered Second Cleavages
  25. 27. Altered Second Cleavages
  26. 28. Synchronous Cleavages
  27. 29. Synchronous Cleavages <ul><li>par-1 and par-4 cleave synchronously up until fourth or fifth cleavage </li></ul><ul><li>par-2 and par-3 are rather earlier </li></ul>
  28. 30. Abnormal Localization of P Granule
  29. 31. Abnormal Localization of P Granule
  30. 32. Abnormal Localization of P Granule
  31. 33. Identify The Phenotype <ul><li>Differentiated Cell Types </li></ul><ul><li>Grandchildless </li></ul>
  32. 34. The Result
  33. 35. Summary

×