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Presented at ImmInst 2010 conference

Published in: Health & Medicine, Business
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  1. 1. HISTORY 1900s – glycerol properties discovered by Russian botanist Maximov 1913 – experiments by Russian physicist Porfiry Bahmetiev on anabiosis and hypobiosis on larvae 1929 – the main character in Mayakovsky play “Bedbug” is cryopreserve 1971 – Russian reanimatologist Vladimir Negovsky discusses “European cryonics corporation” with Anatol Dolinov (France) 2000s – we catch up
  2. 2. OCTOBER 27, 2005 Cryonics case report at our seminar
  3. 3. THE BASIC FACTS Founded 2005, owned by 9 people 15 patients $10K neuro / $30K full-body >50% market share in Europe A for-profit (easier to register and operate in Russia), but a non-profit in spirit 5+ compensated part-time employees, many volunteers
  4. 4. STAFF Valerija Pride, General Director Igor Artyukhov, Science Director Danila Medvedev, Chairman Eugenij Shumilov, Doctor/Technician Alexey Sulaev, Embalmer/Perfusionist Andrey Shvedko, Engineer/Philosopher Victor Grebenshikov, Head of Technical Council
  5. 5. EXAMPLE CASE 1 Patient: 80+ year old relative of a transhumanist Death is in the hospital during the weekend (not in Moscow, but in major city) Delay in notification: 12 hours We arrive ~20 hrs after death The body is cooled passively, core temperature reaches +9C Additional time spent on formalities (due to the weekend): ~15 hrs
  6. 6. EXAMPLE CASE 1 Permission from the head of the hospital is obtained to perform cryopreservation (before the death certificate, so the patient is in custody of the hospital) Head of the pathology department and a nurse assist Our specialist oversees the procedure, prepares the solutions BPS-7 is used as carrier, ramped up glycerol as cryoprotector Access to vasculature is established, washout and perfusion is done manually (up to 50% glycerol
  7. 7. EXAMPLE CASE 1 Cooling is done using water ice and dry ice vapour, nasal temperature monitored Solutions are cooled to a few degrees C During the operation the core temperature is about +2 The brain had to be removed per relatives’ request Cooldown to -80C in a dry ice box with no temperature adjustment (brain submersed in glycerol) The brain is visually inspected, there is no ice formation visible on the surface
  8. 8. EXAMPLE CASE 2 Patient: ~25 year old (in Moscow, several years ago) Delay in reporting: 5 hours. Additional delay: 10 hours In that time we had to find a place to perform perfusion, find a surgeon, assemble the team, get necessary supplies in place, move the patient from the hospital, get necessary permissions Access and perfusion done by surgeon, assistance by our specialist. A perfusion specialist
  9. 9. EXAMPLE CASE 2 Whole body perfusion takes ~7 hours Whole body is preserved in dry ice No way to ascertain the quality of the perfusion (venous return not analyzed for glycerol concentration) A perfusionist analyzing the recording heavily criticized the operation Later cellular studies show good viability in cryopreserved fibroblasts, sperm, other cells. Neurons not studied
  10. 10. EXAMPLE CASE 3 A 70+ year old relative of a transhumanist Death in Moscow hospital (intensive care) Agreement with administration reached pre- mortem Notification about death: <1 hour Access to the morgue in 2-3 hours Medication including heparin is injected by our specialist, indirect heart massage is performed
  11. 11. EXAMPLE CASE 3 The body is additionally cooled with water ice while in morgue The body is taken out of the hospital before government death certificate is issued (only with a medical death certificate) with the help of a funeral director Perfusion takes place in the morgue near KrioRus An embalmer experienced in doing open- circuit embalming perfusion (including 5+ cryonics cases) performs the operation
  12. 12. EXAMPLE CASE 3 A roller-pump is used with open-circuit perfusion The operation is monitored using video- conferencing by a US cryonicist (Mike Darwin) who provides additional advice Two nurses and our specialist assist Blood return samples are taken Head is preserved Cooling is done with manual temperature control and tracking (in an acetone bath)
  13. 13. EXAMPLE CASE 4 An old dog is cryopreserved The operation takes place in a private laboratory A surgeon is performing the operation A closed-circuit perfusion is used (takes 12+ hours for initial setup) The operation starts on a live anesthetized dog There is some difficulty establishing access
  14. 14. FACILITIES
  15. 15. FACILITIES
  16. 16. INTERNATIONAL COOPERATION Can accept neuro and full-body patients for storage Can pick up the patients in Europe, Russia, CIS no standby/perfusion capability in EU Are always willing to share information with local groups (friendships with groups in Finland, South Africa, Australia, Hong Kong, etc.) Will offer EUCRIO standby to our clients
  18. 18. QUESTIONS? www . kriorus . ru kriorus @ +7-911-KRIORUS Moscow, Russia