Overview of pre-breeding w/focus on the use of CWR in potato improvement at CIP M. Bonierbale, E. Mihovilovich, B. Ordoñez...
Pre-breeding GoalAssure the flow of valuable genes and diversityfrom CWR and landrace germplasm intoproductive, improved v...
General Pre-breeding Targets             & Cases for Potato at CIPA.  Traits “missing” from advanced      Ø  Pests, PVY, ...
Searching for traits1.  Crop Wild Relative2.  Cultivated Landraces3.  Broad-based advanced Populations
Progress and prospects of Potato         Improvement by sources and traits                 (CIP at a glance)              ...
Foundation & Early Breeding        Research Contracts
Assets: Raw and Improved Germplasm Adapted to Tropical Agro-ecologies                                                     ...
Sustainability Population Improvement   Progenitors     Incorporation      Progenitors                    New CyclesNobili...
Historical PreBreedingApproaches & Results             LB             BW                         Virus                    ...
Pre-breeding forBW + RKN Resistance                                                                               1977 -19...
Selections from 1990’s Series Show Reduced      BW Severity Under High Pressure
Products of Collaborative BW Resistance            PreBreeding Program            391930.1                                ...
Highly Heterozygous Nematode Resistant Variety      2x Breeding follow by 2x-4x Hybridization                             ...
Strategic Broadening of the Late Blight    Resistance of Advanced PopB3                                                   ...
1. Determine the Architecture of LB                      Resistance in B3 Population               chr1                   ...
2. 2. Search for Novel Resistance Sources:   Evaluate Putative Novel Resistance Sources               Piurana and Tuberosa...
3. Pre-breeding with Putative Novel Resistance Sources                                                                    ...
Approaches for Selecting Complementary          Resistance Sources and Genes                                              ...
Comparative Gene Expression betweenB3 and S. cajamarquense under LB Challenge          Study# 83 at          Solanaceae   ...
Lesson learned•  Interspecific         •     Innovative approaches   reproductive barriers       (Sli?)•  Lack of fertile ...
Lesson Learned•  Variability of      •     Use and monitor   pathogens                 complementary sources•  Accumulatio...
Recomendations•  Develop mechanism to sustain funding for   maintenance of most-valuable prebreeding   populations and sto...
More Pre-breeding Products                      112 rare LB                      resistant                      hybrids wi...
Acknowledgements•  W.Perez, A.Salas, S.Priou, L.Gutarra,
Thank you
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Merideth Bonierbale's presentation in the framework of the expert consultation on the use of crop wild relatives for pre-breeding in potato

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The expert consultation on the use of crop wild relatives for pre-breeding in potato was a workshop organized by the Global Crop Diversity Trust in collaboration with CIP and took place from the 22nd – 24th of February 2012.

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Merideth Bonierbale's presentation in the framework of the expert consultation on the use of crop wild relatives for pre-breeding in potato

  1. 1. Overview of pre-breeding w/focus on the use of CWR in potato improvement at CIP M. Bonierbale, E. Mihovilovich, B. Ordoñez Global Crop Diversity Trust CWR Consultancy: Potato PreBreeding 2/22/2012, Lima, Peru
  2. 2. Pre-breeding GoalAssure the flow of valuable genes and diversityfrom CWR and landrace germplasm intoproductive, improved varieties that enhancesustainable and profitable farming systems.Objectives–  Identify, upgrade, combine and document new sources of needed traits for their incorporation into useful support population and stocks.–  Support the continued utility of CIP’s broad-based tropically- adapted potato populations for variety selection and potato improvement.
  3. 3. General Pre-breeding Targets & Cases for Potato at CIPA.  Traits “missing” from advanced Ø  Pests, PVY, PVX, BW populations and adapted varieties. resistanceB.  Higher levels and more highly heritable sources of needed traits. Ø  PLRV resistanceC.  Complementary sources to build durable resistance to variable Ø  Late blight resistance pathogens.D.  Horizon scanning: Emerging Ø  Heat, salinity and Constraints; Climate Change. drought toleranceE.  Opportunities for new/added-value Ø  Nutritional value traits not previously attended by (micronutrient density) breeding programs.
  4. 4. Searching for traits1.  Crop Wild Relative2.  Cultivated Landraces3.  Broad-based advanced Populations
  5. 5. Progress and prospects of Potato Improvement by sources and traits (CIP at a glance) Before CIPs 40 years CIP History Future foundation 80 85 90 95 00 05 10Late Blight dms ,  A B P TPotato Cyst Nematode(Globodera pallida)FrostPVY, PVX acl,  s toPLRVPotato Tuber MothRoot Knot NematodeEarly BlightBacterial WiltDroughtBioavailable Micronutrients
  6. 6. Foundation & Early Breeding Research Contracts
  7. 7. Assets: Raw and Improved Germplasm Adapted to Tropical Agro-ecologies Lowland phu Tbr Adg tropics and Neo dms Virus Resistant tbr (LTVR) Population dms 1.2 Lesion radius (mm) y = a+bx Tbr 1.0blb phu b=LGR= 5.7 Highland 0.8 0.6 acl Neo Adg tbr 0.4 0.2 y = a+bx b=LGR= 3.6 tropics 0.0 3 5 7 Late Blight Days after inoculation Resistant (B3) Population
  8. 8. Sustainability Population Improvement Progenitors Incorporation Progenitors New CyclesNobilisation and Genetic studies:Introgression of •  Parental value Evaluation •  Heritability new diversity •  Variability Support Yield, Stability Populaciones Advanced populations Adaptation Elite clones
  9. 9. Historical PreBreedingApproaches & Results LB BW Virus PLRV
  10. 10. Pre-breeding forBW + RKN Resistance 1977 -1995 BW, LBdms × tbr (Mex germ:Atzimba) S.  sparsipilum x S.  chacoense tbr (earliness), phu (CCC)x  tbr (Katahdin) (University  of  Wisconsin) Katahdin,  Atlantic,  DTO-­‐28 E S.  microdontum (  CIP,  1977)   S.  phureja BW 2x BR,  MS,  PSP,  PSW Katahdin,  Atlantic,  DTO-­‐28 E, HT HT Stn,  phu.  Gon tbr (heat  tolerance), (Long  adapted   Group  V  (2x) Group  I X M.  tropical,  Serrana germplasm) X MBN  (BW  +  RKN) CGN-­‐69.1 (1977) CGN-­‐69.1 PI/PS  bulk   X (  CIP,  1977)   (NCSU) (1980) Group  II   ‘Nobilisation” Group  III (1979)BW (1979-­‐80) 4x  x  2x   Group  VI  (2x) X MBN  +  TUBEROSA Group  IV (1983) (1980) raph  ,  chc     (AVRDC-­‐1287.19) Group  VII CIP   BW TD  (4x) (1985) X LTVR  population E, HT, V (4x) 4x  population  (1995) Highly  heterozygote Four  sources  of  BW  resistance  +  virus   resistance  
  11. 11. Selections from 1990’s Series Show Reduced BW Severity Under High Pressure
  12. 12. Products of Collaborative BW Resistance PreBreeding Program 391930.1 394904.17 BW, LB, PVX, BW, High high concentration concentration Zinc and Vit C Iron and Zinc 394908.13 395443.103 BW, PVX, high concentration BW, LB –HR, PVX, Zinc high concentration Zinc and Vit C 395446.1 BW, PVX, PVY, High concentration Iron and Zinc
  13. 13. Highly Heterozygous Nematode Resistant Variety 2x Breeding follow by 2x-4x Hybridization vrn 2488-A (C.P.C. collection) 82M124.27 HT Bulk - stn-phu (NCSU) 84.28.42 stn-phu (NCSU) 378908.43 381343.40 (MBN) S. spl Bulk Kura CIP 760147.7 85.27.13 (2x) (Pi 1230502) (CIP PTL 80M (virus) 591099.13) MI-7.10 stn-phu (NCSU) BW 84.194.11 S. spl (MBN)Nemared CIP 760147.7 MI-49.10 (Pi 1230502)387559.3released in 1994 AQUILA [tbr x (tbr x phu)] LT-1 KATAHDIN (tbr) LT-8 (4x) PVXY Bk. (adg, neo-tbr)
  14. 14. Strategic Broadening of the Late Blight Resistance of Advanced PopB3 1.2 Lesion radius (mm) y = a+bx 1.0 b=LGR= 5.7 B3 0.8 0.6 Population 0.4 y = a+bx 0.2 b=LGR= 3.6 S bulbocastanum S. .tuberosum 0.0 S phureja 2x=48 3 5 7 2x=24 S acaule Days after inoculation 2x=24 4x=48 S. demissum 2x=48 S. tuberosum 25 S. andigena Number of families 2x=24 2x=48 20 15 10 B3C3 5 B3C0 0 AUDPC (Family Means)
  15. 15. 1. Determine the Architecture of LB Resistance in B3 Population chr1 chr2 chr3 chr4 chr5 chr6 EatgMcag80 0.0 STI0024f 0.0 EaacMcac205 0.0 STMGX55 0.0 STI0032f 0.0 EatgMacg249 0.0 STM5127f 4.2 STG0006f 7.5 EacaMcag51 1.3 STG0020 10.5 EaaaMacg340 6.7 T0892_COS6f STM1008 9.8 9.9 STI0034 10.8 STM3016 11.4 At5g14320 11.8 At3g10920f 10.2 STM5114f 10.7 EaacMcag243 16.2 EaacMcac167 12.3 STM0019 16.0 STI0043f 17.7 STI0029f 20.3 At3g58490f 20.8 STI0036f 21.0 At5g51110f 26.5 STM5140 21.8 STM5119 21.4 At1g29520 23.4 At5g12370 28.3 EaaaMcag328 25.8 EaacMcac244 At1g44575f 28.2EaacMcac560 32.0 29.0 STG0033 32.7 At1g02140 At1g44760 30.2 STM1029f 33.1 30.3 STI0031f 34.2 EaaaMacg246 32.0 At1g52200 36.7 T0869_COS2.1 38.6 STM0020f 39.4 STI0013f1 35.7 STM1100 40.2 GP25b1895 36.7 STG0021 48.8 dPI02 STI0013f2 37.8 T1169f 48.2 EagaMcat391 50.2 STI0049f 50.4 EacaMcag96 51.8 STG0016f 53.4 At5g24120f2 38.8 STPOAC58b86 58.5 At1g51160f STG0008 54.7 STI0004f 53.9EatgMacg528 54.7 STM0038 57.4 39.6 EaaaMacg191 59.6 STM1009f2 59.9 At5g53490 42.3 EaaaMacg86 61.8 At3g02910f 49.1 STM5149 65.1 At5g41040f 50.9 At5g26360 66.2 At1g07020 54.5 STM3011 77.1 STI0050f 57.1 STG0022 61.1 STI0012 80.0 STM5115 75.5 STM2030 88.4 At1g16080 76.8EacaMacg829 89.5 GP198b1572 86.1 STM1049 90.6 STG0010 92.4 chr7 chr8 chr9 chr10 chr11 chr12 STLSIG239 dPI07 0.0 STM0024 0.0 STM1102f 0.0 STM5132f 0.0 STM0037 0.0 STM5121 0.0 STM3009f 3.1 LEUZIP119 2.6 STI0002f 2.4 STI0023f 1.4 STG0004 1.1 dPI09a STM0028 4.2 STI0038 3.9 STM1051 STM1106b 10.4 4.6 STM1057f1 14.6 STI0057f 7.9 STI0019f 21.7 STMIP26f STM3012 10.4 23.1 STM2013f 22.5 STM1057f2 EagaMcat323 20.0 27.1 STI0028f 25.8 STM0030f STM0010 29.8 27.2 STI0027f 27.8 STM5109 29.9 STM0017_172 31.6 STI0030f 31.4 At3g26060 35.9 STI0003 36.4 STG0015 STM2005f 32.8 35.5EaacMcac382 37.1 STI0022b127 37.7 EagaMcat196 35.0 STI0044b310 39.0 EaacMcac700 43.0 STM0031f 46.8 At4g11600 47.1EaaaMacg451 47.8 STI0046b547 51.2 STI0040f 52.2 STWAX-2f 53.5 STGBSS-1f STINHWI228 64.4 STM0025f 53.1EagaMacg582 55.5 54.7 STMIR63 57.2 STM1104f 58.1 STI0014 66.8 STM1021f 68.2 STMCL13 63.5 dPI09b At2g24270 65.5 dPI12 STINHWI277 69.2 STG0007 66.3 At3g24160f2 76.2 EaacMcac368 71.4 DMC42152bf 77.6 STM0003f 72.8 dPI09c PI09 DMC42144af 78.8 STI0051f 77.7 Rpi_svnt1_367 81.0 At3g24160f1 85.6
  16. 16. 2. 2. Search for Novel Resistance Sources: Evaluate Putative Novel Resistance Sources Piurana and Tuberosa Groups Field and Detached leaf Assays S. chiquidenum Comas. Oxapampa 2000-2004 S. paucissectum S. piuraeS. cajamarquense
  17. 17. 3. Pre-breeding with Putative Novel Resistance Sources Embryo Rescue Technique Prezygotic barriers Field Testing & Selection 4x × 2x (B3 clones x ER) Field testing2000 2001 2002 2003 2004 2005 2006 2007 2008 2009 2010 2011 2012 2013 Selection: 3rd resistance + 2nd agronomic + Project start Crossing Blocks reproductive 1st Selected wild 2x Pre Breeding populations traits. Dihaplod Native 2x selections PF A B C D PM wild wild x = x A B C D Diallel wild x wild cross Hybridity test Field Testing and selection
  18. 18. Approaches for Selecting Complementary Resistance Sources and Genes phu-703308 B3-303946.7 Chr11 Oxapampa2005 Avr-vnt1 Comas 2001 + -LOD Avr-vnt1+ Rpi-vnt1 + + Rpi-vnt1 Rpi-pcs N, NL25, R3, R3a, b, - - NL27, R6, R7 Empty Bs4, R10, R11 vector - -
  19. 19. Comparative Gene Expression betweenB3 and S. cajamarquense under LB Challenge Study# 83 at Solanaceae Gene expression database: http://www.tigr.org/tdb/potato/ SGED_index2.shtml Design :advanced B3 S. cajamarquenseclone (cjm) Inoculation with isolates POX067 and PE B3 274Sample collection at 72h and 96h after 30 inoculation cjm 329Hybridization to the TIGR10K potato array
  20. 20. Lesson learned•  Interspecific •  Innovative approaches reproductive barriers (Sli?)•  Lack of fertile •  Selection & recipients w/ good tuber appearance consolidation of pool of compatible recipients•  Dilution of resistance •  Sufficient during breeding recombination; Monitor process w/ molecular tools.•  Poorly-suited genetic •  Attention to adaptation background of recipient germplasm
  21. 21. Lesson Learned•  Variability of •  Use and monitor pathogens complementary sources•  Accumulation of •  Monitor glycoalkaloids glycoalkaloids accumulation during breeding process•  Large populations and cumbersome •  Rapid and affordable screening methods screening methods•  Limited, short term •  Develop and debate project funding business models •  Capitalize products per se? Courses?
  22. 22. Recomendations•  Develop mechanism to sustain funding for maintenance of most-valuable prebreeding populations and stocks•  Document, communicate and create demand for products for use in house and across institutions•  Develop Community of Practice for potato pre-breeding
  23. 23. More Pre-breeding Products 112 rare LB resistant hybrids with 2n pollen and good agronomic performance (ER)
  24. 24. Acknowledgements•  W.Perez, A.Salas, S.Priou, L.Gutarra,
  25. 25. Thank you

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