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Introducing
Generating nitrogen on location:
the power behind the Anoxia technique
For more information: www.n2gf.com

What is the
connection between
nitrogen production
and animal welfare?
Nitrogen generation on location is a critical
component of the Anoxia technique
The anoxia technique safeguards animal
welfare during outbreaks of animal diseases
Mobile Nitrogen technique is now available
world wide thanks to the corporation with
the Gazcon/Atlas Copco organization.
Disease outbreaks
threaten the lives of
millions of animals
every year.
Outbreaks need to be
contained before they
become a major threat
to entire nations.
The Anoxia technique
is the 21st century
solution in the battle
against contagious
animal diseases
More information: www.n2gf.com

Techniques that change the world
What is the
Anoxia
technique?
Why use a
nitrogen
generator?
What is the
importance?
The first time that nitrogen is used to guarantee animal welfare
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Anoxia What is it?
Presented by the N2GF team
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Anoxia: Never heard about it?
• In a plane you are instructed what to do in
case the cabin pressure drops ..
• When climbing Mount Everest, you need
additional oxygen, unless you are a Sherpa ..
• In case you are diving, you are instructed
what to do when you dive below 30 meters
..
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Anoxia: How does influences humans?
– Oxygen must be present in every breathing gas
because it is essential to the human body's
metabolic process
– As the altitude increases (from > 2,400 meter)
atmospheric pressure decreases, oxygen levels drop
– At 30 meters below sea level atmospheric pressure
is 4 times the normal atmosphere, leading to higher
CO2 and lower Oxygen levels.
– A decrease in blood oxygen level leads to relative
increase of the nitrogen concentration inhaled,
resulting in Hypoxic Anoxia
– In the medical literature, the effects of exposure to
high concentrations of nitrogen is described as
Nitrogen Asphyxiation
6
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Hemoglobin: its crucial role in the transport
of gasses through the body
• Hemoglobin is the ironcontaining oxygen-transport
metalloprotein in the red blood
cells of all vertebrates
• Hemoglobin enables the
transport of gasses throughout
the body
• It carries oxygen from the
respiratory organs (lungs) to the
rest of the body where it
releases the oxygen
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Anoxia caused by exposure to >98% Nitrogen:
What’s happening?
• No physical responses; the air we breath
consists of 78% Nitrogen, so high quantities
of remain undetected ..
• No stress or pain sensations, on contrary;
mountain climbers and divers describe
Anoxia as reaching a state of positive anxiety
..
• No reaction by animals when they are
exposed to Anoxia until the moment that
they turn unconscious ..
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The technical breakthrough:
Fill large low expansion foam bubbles with 100% Nitrogen
• Invented in Holland in 2003, as alternative method for existing
CO2 techniques used for mass killing animals during outbreaks
• Since 2006 successfully tested as killing method of poultry by
scientists in Holland, The UK and the USA
• The anoxia technique contributes to todays economic, social,
environmental, public health and consumer welfare objectives
9
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The Anoxia technique:
the perfect killing and slaughter technique?
Yes
but there are obstacles:
• Scale: The Anoxia technique needs to be available on a world
wide scale to be effective against disease outbreaks
• Sepsis: Prior attempts to create anoxia by using nitrogen has
been proven to be technically complex and expensive
• Proof of Concept: Like for all new slaughter techniques, this
technique is subject to comprehensive testing to verify animal
welfare claims
10
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The Anoxia technique:
What’s EU stance towards anoxia?
Positive
• Since 2006, Anoxia is successfully tested as onsite slaughter
technique, in case of large-scale outbreaks of animal diseases
• Starting January 2013, the European Union accepted Anoxia
as slaughter method for
• Pigs
• Poultry
• In Holland, Anoxia is tested on its practicality to euthanize sick
and crippled piglets and poultry on the farm
11
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The Anoxia technique: How to apply it?
High expansion foam filled with >98% Nitrogen
• Animals are submerged into foam, filled
with 99,8% Nitrogen
• After 30 seconds, the animals reaches a
state of unconsciousness
• The animal dies within 1,5 minute on the
consequences of Anoxia
12
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Killing and slaughtering animals
Starting Junuary1, 2013, Directive EU 1099/2009
• Is valid in all EU Member states where animals are killed or
slaughtered:
– Slaughtering of animals at the slaughter house
– Killing of animals for depopulation purposes during outbreak situations
– Killing for welfare purposes of sick and crippled animals on the farm
• Only animal-specific methods may be applied; manually
killing of animals (pulling the neck, smashing the scull etc.) is
considered a crime within the EU
13
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Allowed animal-specific methods:
1.
2.
3.
4.
All animal species
Mechanical methods
Electrical methods
Gas methods (hypoxia, hypoxemic
anoxia, anoxia)
– Carbon dioxide at high
concentration
– Carbon dioxide in two phases
– Carbon dioxide associated with inert
gases
– Inert gases
– Carbon monoxide (pure source)
– Carbon monoxide associated with
other gases
Other methods
– Lethal injection (T61)
14
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Anoxia Why use a Nitrogen
Generator?
The first time that nitrogen is used to guarantee animal welfare
For more information: www.n2gf.com

Reason Nr 1:
It makes the Anoxia technique
applicable where you need it
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Nitrogen foam: What’s so special?
• It is the first universal slaughter technique
that can be applied to all animal species
• It can be applied during outbreak situations,
on farms, as stunning technique in slaughter
houses
• It takes not more than 30 seconds to create
the volume of 1 M3 Nitrogen filled foam per
nozzle
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Nitrogen foam: Why a Nitrogen generator?
Using Nitrogen generators makes the production
of gas on location possible:
• 50% of all pigs in Europe, are stunned using
CO2 , using large quantities of gas. Compared
with the Anoxia technique only a fraction of
gas quantity is needed, significantly reducing
the operational costs.
• Large quantities of liquid CO2 are used to flood
entire stables during outbreak situations. The
Anoxia method replaces this CO2 stable gas
technique completely
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Anoxia: What’s the
importance?
The first time that nitrogen is used to guarantee animal welfare
For more information: www.n2gf.com

Urgent need for new, technical solutions
Demand for Humane on-farm euthanasia
techniques
In average, 3% of all farm animals get sick
or injured and need to be treated or
euthanized
Using brute force to kill animals is is strictly
forbidden and considered as a crime
Demand for mobile slaughter techniques
In average, every 3th week, there is a new
outbreak of animal disease somewhere on
earth
Within the EU alone, there are more than
2.2 million farm locations that need to apply
to the new EU directive
For most animal species, mobile slaughter
techniques do not exist
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Without techniques, diseases can’t be stopped
1997:

2001:
Holland: financial impact Classic Swine fever outbreak
€ 2,8 billion
UK: Financial impact
Foot & Mouth Disease FMD outbreak

2003:
€ 15.1 billion
Holland financial impact
Avian Influenza AI outbreak

2011:
€ 1,275 billion
South Korea: Financial impact
Foot & Mouth Disease

2010:

€ 2,1 billion
Japan: financial impact FMD/AI outbreak:
€ 1,16 billion
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The world needs techniques to slaughter animals on
location
All pictures are taken within the last 10 years.
We need to think outside the box to prevent these animal cruelties in the future
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Transporters need techniques to euthanize animals
No solutions for shipping of sheep
Every year, 6,5 million sheep
are exported from Australia
to Africa and the Middle
East.
On average, 8% of all sheep
get sick, infected or cripple
during their transportation
from Australia to the Middle
East.
These ships are not
equipped with mobile
killing equipment.
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What’s needed are mobile slaughter techniques
The Anoxia method is the first
technique that can be applied to
all animal species
What’s needed are:
•
•
•
•
•
Mobile nitrogen generator
Foam nozzles
Tap Water
Soap
Trained staff
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The first time nitrogen generators are
used to guarantee animal welfare
Contact us for more information
The first time nitrogen generators are
used to guarantee animal welfare
For more information: www.n2gf.com