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AVT session 6: The science behind the Anoxia technique

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This sixth presentation of a series of 6 AVT presentations on the science behind the Anoxia technique.

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AVT session 6: The science behind the Anoxia technique

  1. 1. Harm Kiezebrink Research Fellow Faculty of Veterinary Science Queensland University
  2. 2.  In a plane you are instructed what to do in case the cabin pressure drops ..  When climbing Mount Everest, you need additional oxygen, unless you are a Sherpa ..  In case you are diving, you are instructed what to do when you dive below 30 meters .. Anoxia: Never heard about it? © 2014 Applied Veterinary Technologies Europe AB 2
  3. 3. The metabolic process  As the altitude increases (from > 2,400 meter) atmospheric pressure decreases, oxygen levels drop  At 30 meters below sea level atmospheric pressure is 4 times the normal atmosphere, leading to higher CO2 and lower Oxygen levels.  A decrease in blood oxygen level leads to relative increase of the nitrogen concentration inhaled, resulting in Hypoxic Anoxia or Nitrogen Asphyxiation © 2014 Applied Veterinary Technologies Europe AB 3
  4. 4. Hemoglobin  Hemoglobin is the iron- containing oxygen-transport metalloproteinase in the red blood cells of all vertebrates  Hemoglobin enables the transport of gasses throughout the body  It carries oxygen from the respiratory organs (lungs) to the rest of the body where it releases the oxygen © 2014 Applied Veterinary Technologies Europe AB 4
  5. 5.  No physical responses, the air we breath consists of 78% Nitrogen, so high quantities of remain undetected ..  No stress or pain sensations, on contrary; mountain climbers and divers describe Anoxia as reaching a state of positive anxiety ..  No reaction by animals when they are exposed to Anoxia until the moment that they turn unconscious .. Anoxia caused by exposure to >98% Nitrogen © 2014 Applied Veterinary Technologies Europe AB 5
  6. 6. Proof of principle Anoxia method  Published on May 2013 in Poultry Science 92:1145–1154  Reviewed/downloaded 21.500 times on N2GF.com  Main researchers  Dorothy McKeegan - College of Medical, Veterinary & Life Sciences, University of Glasgow  Marien Gerritzen - Wageningen UR, Livestock Research  Main conclusion  “These trials provide proof-of- principle that submersion in gas-filled, high expansion foam provides a rapid and highly effective method of euthanasia, which may have potential to provide humane emergency killing or routine depopulation. “ © 2014 Applied Veterinary Technologies Europe AB 6
  7. 7. Heart rate responses © 2014 Applied Veterinary Technologies Europe AB 7
  8. 8. EEG responses © 2014 Applied Veterinary Technologies Europe AB 8
  9. 9. ECG responses © 2014 Applied Veterinary Technologies Europe AB 9
  10. 10. Behavioral responses © 2014 Applied Veterinary Technologies Europe AB 10
  11. 11. Foam parameters group trial © 2014 Applied Veterinary Technologies Europe AB 11
  12. 12. Behavioral responses Group trial © 2014 Applied Veterinary Technologies Europe AB 12
  13. 13. Results summary group trials © 2014 Applied Veterinary Technologies Europe AB 13
  14. 14. Results summary group trials © 2014 Applied Veterinary Technologies Europe AB 14
  15. 15. Proof of principle Conclusions © 2014 Applied Veterinary Technologies Europe AB 15
  16. 16. Question and Answer Reflection: What’s on your mind?  16 16 © AVT Applied Veterinary Technologies Europe AB Address details: c/o INTRED, Södra Hamnen 2, 45142 Uddevalla, Sweden Org.nr: 556792-1944 - Phone: +44 7452 272 358- E-mail: harm.kie@gmail.com

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