Telomeres and senescence in sockeye salmon

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Presentation for the 2010 Alaska Salmon Program Symposium

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Telomeres and senescence in sockeye salmon

  1. 1. Rapid senescence in sockeye salmon<br />Insights from telomeres<br />Caroline Storer<br />Tom Quinn <br />Steven Roberts<br />Alaska Salmon Program Symposium 2010<br />
  2. 2. Senescence in sockeye salmon<br /><ul><li> Adaptive variation of senescence within populations
  3. 3. Significant variation in in-stream longevity (i.e., rate of senescence) among populations
  4. 4. Links to other aspects of fitness such as energetic investment in gonads versus body stores (Hendry et al. 2004)</li></li></ul><li>What physiological processes regulate senescence in sockeye salmon?<br />
  5. 5. Study site<br />
  6. 6. Previous research (2008-2009)<br /><ul><li> Baseline information on physiological changes between “arriving” and senescent salmon
  7. 7. Cortisol signaling declines during senescence
  8. 8. Molecular chaperone protein (HSP) is up-regulated during senescence
  9. 9. Molecules controlling “other” primary process are differentially expressed (immune, reproduction, olfaction, and memory)</li></li></ul><li>New approach<br />Examine new physiological process:<br />
  10. 10. New approach<br />Examine new physiological process:<br />Telomere shortening<br />
  11. 11. Why telomeres?<br />
  12. 12. Why telomeres?<br /><ul><li> Telomeres shorten in lengthwith age
  13. 13. Telomere shortening is significantly correlated with decline in physiological health (Terry et al. 2008)
  14. 14. Telomere length can be inherited (Atzmon et al. 2010)
  15. 15. Critical telomere length could be one possible trigger of senescence (Armanios 2009)</li></li></ul><li>New question<br />Doestelomere shortening directly trigger senescence and/or regulate rate of senescence in sockeye salmon?<br />
  16. 16. Samples (2010)<br /><ul><li>Fin clips and tagging of arriving fish
  17. 17. Early and late arriving fish
  18. 18. Fin clips of tagged fish at multiple “in-stream” time points (i.e. day 2, day 6)
  19. 19. http://goo.gl/tCeJ7</li></li></ul><li>New methods<br /><ul><li> High-throughput PCR based fluorescent assay for the normalized measurements of relative telomere length</li></li></ul><li>New methods<br /><ul><li> High-throughput PCR based fluorescent assay for the normalized measurements of relative telomere length</li></ul>Fluorescence <br />Log Telomere Length<br />
  20. 20. Preliminary results<br />
  21. 21. Summary and next steps<br /><ul><li> We can measure telomere length
  22. 22. However, assay needs to be optimized for greater sensitivity</li></li></ul><li>Summary and next steps<br /><ul><li> We can measure telomere length
  23. 23. However, assay needs to be optimized for greater sensitivity
  24. 24. Is there a difference in telomere length between early (slow senescing) and late (fast senescing) arriving fish?
  25. 25. Does telomere length shorten during rapid senescence?</li></li></ul><li>Implications<br /><ul><li> Provides insight into the evolution of life history strategies and phenotypic variation in longevity
  26. 26. Rapid senescence in sockeye salmon could be used a model to study aging and longevity in other organisms</li></li></ul><li>Acknowledgments <br /><ul><li> The 2010 sampling crew, especially Curry Cunningham, Jackie Carter, Brian Quinn, and Wes Larson
  27. 27. Leslie Jensen
  28. 28. The Alaska Salmon Program
  29. 29. Roberts Lab
  30. 30. Seeb Lab</li>

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