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Adipose-derived stem cells (ADCSs) are isolated from subcutaneous adipose tissue (a suitable reservoir of stem cells). These cells have the potential to differentiate in vitro into chondrocytes, osteoblasts and adipocytes and have shown their ability to heal wounds and regenerate tissues in clinical trials. ADSCs are isolated from a single animal and cultured until passage 1, and then cryopreserved. Post-thaw viable cells are tested for proliferation and differentiation capacity into adipocytes, osteocytes and chondrocytes.