Spectroscopy allows scientists to calibrate the linear relationship between the concentration of a substrate and its absorbance as measured by a spectroscope. A series of dilutions were prepared with varying quantities of substrate and substance to obtain different molarity solutions. Absorbency numbers from the spectroscope and concentration numbers were used to establish a linear calibration curve relating concentration to absorbance. This calibration curve allows scientists to determine the unknown concentration or molarity of a substance if one of the values is known.

1. Spectroscopy-Calibration Curve<br />Spectroscopy is the science that studies the interaction of matter and the electromagnetic field. In the realm of science, we can calibrate and establish a linear relation between the concentration of a substrate and its absorbance as calculated with the spectroscope. The spectroscope is a machine that calculates a physical or chemical characteristic as a number. This allows scientists to have better results and more accuracy in their procedures. First, we prepared a series dilution. We did a daughter solution from a mother solution, and then we continue diluting. We also varied the quantity of substrate and substance. This provided us a different molarity in each solution. Then we put the solution in the spectroscope and obtained the absorbency number. With concentration number and the absorbency number, we established a mathematical linear relation between the concentration and the absorbency using calibration curve. The information indicated that if you only have the quantity and the molarity of one of the substances then, using the linear regression formula, you can know the molarity or concentration of the substrate. <br />Carlos Santos Perez<br />804-10-8280<br />University of Puerto Rico<br />Cayey P.R.<br />