Microbiology review
• 8. What does “parfocal” mean? Is the  microscope used in the laboratory a “parfocal”  microscope? Parfocal is that a mic...
• Know the difference between gram + and –• Know what the different reagents in the gram  stain and what they accomplish.•...
• Know all the media we used and what positive  test means and what a negative test means.  Also when a organism doesn’t g...
•   Macconkey agar•   Positive means•   If it grows it means•   EMB•   If it grows ?•   If is positive?
•   MSA (mannitol salt agar )•   Positive means ?•   Growth Means ?•   Mitis – salavarius•   Colonies are blue?•   If no g...
Stains•   Positive stain•   Stains the cell also known as the basic stain•   Negative stain•   Stains all the back ground ...
Gram stain•   Gram stains•   Primary stain•   Crystal violet•   Mordant•   Grams iodine•   Decolorizer•   95% ethanol alco...
Gram negative Media• EMB• Macconkey agar• Hektoen• Urea
EMB• A positive test means what ?• growth of Gram + organisms. Additionally, the  medium contains lactose which can be uti...
EMB
Macconkey’s• What does growth mean ?• That is a gram -• What does positive test mean?• above pH 8. Like EMB, this medium c...
Macconkey agar
Hektoen enteric agar• MacConkey’s agar. However, this differs from  MacConkey’s agar because it also has ammonium  sulfate...
Hectoen enteric agar
Urea broth•   If its positive•   the organism has urease .•   If negative it doesn’t poses urease.•   Has phenol red as a ...
Urea broth
Gram positive• MSA (man• PEA• Mitis-salavarius
Mitis-salavarius• Grows up mainly gram positive mainly  streptococcus.• If the colonies turn blue what does this mean?• It...
Mitis- salavarius
PEA• This is a selective media it doesn’t allow  which type of bacteria to grow?• Gram negatives.• What is incorporated in...
PEA
Mannitol salt agar• IF an organims grows this means that the  organism is a halophile.• If it changes color which is a pos...
Mannitol salt agar
Microbiology review
Microbiology review
Microbiology review
Microbiology review
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Microbiology review

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Microbiology review

  1. 1. Microbiology review
  2. 2. • 8. What does “parfocal” mean? Is the microscope used in the laboratory a “parfocal” microscope? Parfocal is that a microscope stays in focus when you change the objective lens.• Parcentric?• Stays center even after you change the focus.• Whats your TA’s Name and where to fid him.
  3. 3. • Know the difference between gram + and –• Know what the different reagents in the gram stain and what they accomplish.• Know what a basic (positive) and a acidic(negative) stain look like. And what they stain.
  4. 4. • Know all the media we used and what positive test means and what a negative test means. Also when a organism doesn’t grow what that means.• Know selective and deferential definitions.
  5. 5. • Macconkey agar• Positive means• If it grows it means• EMB• If it grows ?• If is positive?
  6. 6. • MSA (mannitol salt agar )• Positive means ?• Growth Means ?• Mitis – salavarius• Colonies are blue?• If no growth?• PEA• Grow means?
  7. 7. Stains• Positive stain• Stains the cell also known as the basic stain• Negative stain• Stains all the back ground not the cells themselves.• Differential stain?• Differentiates the cells some how due to a structure or some type of phenotype characteristics.• Capsule stain uses what 2 types of stains• Manevals stain• positive stain.• Congo red• Negative• Endospore stain uses what stains• Malachite green and safernin
  8. 8. Gram stain• Gram stains• Primary stain• Crystal violet• Mordant• Grams iodine• Decolorizer• 95% ethanol alcohol• Counter stain• Saferin
  9. 9. Gram negative Media• EMB• Macconkey agar• Hektoen• Urea
  10. 10. EMB• A positive test means what ?• growth of Gram + organisms. Additionally, the medium contains lactose which can be utilized by lactose fermenters as a carbon and energy source.• Grow means its what ?• That it’s a gram -.
  11. 11. EMB
  12. 12. Macconkey’s• What does growth mean ?• That is a gram -• What does positive test mean?• above pH 8. Like EMB, this medium contains lactose and those organisms that can utilize lactose produce acids. The acid production results in a lower pH in the medium and a change in the color of the medium to yellow. Neutral red is the ph indicator• The selective molecule is bile salts
  13. 13. Macconkey agar
  14. 14. Hektoen enteric agar• MacConkey’s agar. However, this differs from MacConkey’s agar because it also has ammonium sulfate and sodium thiosulfate in it.• These chemicals are indicator molecules because they react with H2S released from the breakdown of peptones in the medium and turn the H2S producing colonies black (indicating that H2S was present).• The pH indicator in this medium is bromothymole blue.• When bacteria use the lactose and sucrose in the medium acid is produced and the medium’s color changes from a blue/green• It has bile salts as the selective indicator molecule
  15. 15. Hectoen enteric agar
  16. 16. Urea broth• If its positive• the organism has urease .• If negative it doesn’t poses urease.• Has phenol red as a ph indicator
  17. 17. Urea broth
  18. 18. Gram positive• MSA (man• PEA• Mitis-salavarius
  19. 19. Mitis-salavarius• Grows up mainly gram positive mainly streptococcus.• If the colonies turn blue what does this mean?• It incorporates the trypan blue from taking in sucrose peptones and all the different carbohydrates.
  20. 20. Mitis- salavarius
  21. 21. PEA• This is a selective media it doesn’t allow which type of bacteria to grow?• Gram negatives.• What is incorporated in to this to allow only gram positives to grow.
  22. 22. PEA
  23. 23. Mannitol salt agar• IF an organims grows this means that the organism is a halophile.• If it changes color which is a positive test this means that the organism uses mannitol as its main carbon source.• has phenol red indicator.• It has NaCl as a inhibitory molecule.
  24. 24. Mannitol salt agar

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