Ria0

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Ria0

  1. 1.  Availability ◦ Synthetic ◦ Natural – Produced From Animals ◦ Monoclonal Antibodies  Purity – Competing Reactions with Impurities  Stability – Store in Albumin Serum  Specificity – Binding Constant
  2. 2.  Must Have Similar Binding Properties as Unlabeled Ligand  Internally Labeled Ligand ◦ 14 C and 3 H  Externally Labeled Ligand ◦ 131 I and 125 I  By-Products or Incomplete Synthesis ◦ Purification by chromatography (gel filtration)
  3. 3.  Electrophoresis  Gel Filtration  Adsorption Chromatography  Fractional Precipitation ◦ Centrifugation ◦ Filtration  Partition Chromatography ◦ Dialysis
  4. 4.  Enzymeimmunoassays (EIA) ◦ millimolar  Fluoroimmunoassays (EIA) ◦ micromolar  Radioimmunoassays (EIA) ◦ nanomolar to picomolar
  5. 5.  Endocrinology ◦ Insulin, HCG, Vasopressin ◦ Detects Endocrine Disorders ◦ Physiology of Endocrine Function  Pharmacology ◦ Morphine ◦ Detect Drug Abuse or Drug Poisoning ◦ Study Drug Kinetics
  6. 6.  Epidemiology ◦ Hepatitis B  Clinical Immunology ◦ Antibodies for Inhalant Allergens ◦ Allergy Diagnosis  Oncology ◦ Carcinoembryonic Antigen ◦ Early Cancer Detection and Diagnosis
  7. 7.  Based on Simple Biochemistry Principles  Establish Ideal Binder and Ligand  Synthesize Tracer Ligand  Separation of Bound and Free Parts  High Precision and Sensitivity  Powerful Applications to a Wide Range of Medical Fields

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